Ultrastructural features in chronic non‐A, non‐B (NANB) hepatitis: A controlled blind study

Bamber, M.; Murray, Alice M.; Lewin, Jackie; Thomas, Howard; Sherlock, Sheila

doi:10.1002/jmv.1890080407

Cited by 14 publications

(8 citation statements)

References 19 publications

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“…The findings of the Leuven group (20), which we have confirmed, suggest that this change is associated, as it is in chimpanzees, with characteristic nuclear particles which seem not to be virus. Similar electron microscopic nuclear alterations have been found in several other studies in man (27)(28)(29)(30). The tubular cytoplasmic lesion observed in chimpanzees (3 1) has thus far been noted only by few observers (30, 32) in man.…”

Section: Discussionsupporting

confidence: 80%

Histologic Observations in Human Hepatitis Non-A, Non-B

et al. 2007

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Specimens from 57 patients with acute or chronic hepatitis non-A, non-B (HNANB), mostly mild to moderate, were studied by light and, in 22 instances, by electron microscopy. They were compared to specimens of hepatitis A and hepatitis B on file or obtained simultaneously with the HNANB cases. In the HNANB material, two types of light-microscopic lesions were noted in the lobular parenchyma singly or in combination. One was an eosinophilic alteration of the hepatocytic cytoplasm associated with many acidophilic bodies. Conspicuous alteration were often accompanied by microvesicular steatosis. This lesion was associated with nuclear particles detectable by electron microscopy. The second lesion consisted of sinusoidal cell activation which was prominent in some cases. From experiences with chimpanzees, these features were tentatively considered to be stages of the same process. Both lesions were associated with portal and usually mild periportal inflammation which was not related to the degree and type of parenchymal reaction. They were also observed in chronic stages, making the differentiation between acute and chronic HNANB difficult. Active hepatitis B seems to differ from HNANB primarily by the conspicuous presence of lymphocytes in the lobular parenchyma often in contact with hepatocytes, acidophilic bodies, and a more uniform portal inflammatory reaction. In hepatitis A, hepatocellular injury was conspicuously periportal. In HNANB, a cytopathic effect rather than lymphocytic immune attack appears to be of greater significance than in the other forms of viral hepatitis. The recurrence of lobular mainly eosinophilic changes may explain chronicity.

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Section: Discussionsupporting

confidence: 80%

Histologic Observations in Human Hepatitis Non-A, Non-B

et al. 2007

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“…Circular cytoplasmic structures resulting from apposition of two cisternae of endoplasmic reticulum have also been described by Busachi et a1 [1981] in liver biopsies from patients with chronic non-A, non-B hepatitis, but it was emphasized that the typical tubular structures found in chimpanzees were not observed in any of their patients. In other studies, cytoplasmic tubular structures were not detected in hepatocytes of patients with non-A, non-B hepatitis [Gmelin et al, 1980;Bamber et al, 1981;Cabral et al, 1981;Gibo et al, 1982;Kunze et al, 19821. However, cytoplasmic tubular alterations similar to those observed in chimpanzees with non-A, non-B hepatitis have been found in lymph nodes, lymphocytes, monocytes, and tissue macrophages of patients with the acquired immune deficiency syndrome [Sidhu et al, 1983;Schaf et al, 1983;Orenstein, 1983;Ewing et at, 19831 and in circulating lymphocytes and lymph nodes of patients with persistent lymphadenopathy and the acquired immune deficiency syndrome [Anderson et al, 19841. Several laboratories have also reported virus-like particles in liver biopsies of patients with non-A, non-B hepatitis.…”

Section: Introductionmentioning

confidence: 92%

Intracytoplasmic inclusions in human hepatocytes in non‐A, non‐B hepatitis: An ultrastructural study

Tf¹,

Tovey²,

Mg³

et al. 1984

Journal of Medical Virology

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An ultrastructural study was carried out on 114 liver biopsies obtained for diagnostic purposes from patients with various pathological disorders of the liver including hepatitis B-related liver disease, non-A, non-B hepatitis, alcoholic liver disease, fatty change, and cryptogenic cirrhosis. The opportunity was taken to evaluate the significance of intracytoplasmic crystalline structures found in the hepatocytes of nine patients with a variety of liver disorders. The cytoplasmic inclusions varied in size up to 2 microns in length and shape and were not limited by membranes. The presence of these inclusions cannot, however, be correlated either specifically with non-A, non-B hepatitis or with other known nonviral liver disease. The functional, physiological, and pathological significance of the crystalline structures remain to be elucidated.

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“…The assumption that the patient suffered from a simultaneous infection by NANB agents and HBV was supported by findings on the electron microscopic level. In the same specimen core particles of the HBVand aggregates of nuclear particles regarded as characteristic for human NANBH (9,11,12,13,14) could be demonstrated in separate liver cells. The specificity of these nuclear inclusions with a diameter of 20-27 nm, however, has recently been questioned by investigators who found them in tumors of non-hepatic origin (15).…”

Section: Discussionmentioning

confidence: 83%

Simultaneous infection with non‐A, non‐B and B viruses in a patient exhibiting different histologic patterns of acute hepatitis

Dienes¹,

Grün²,

Heß³

et al. 1983

Liver

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We present the case of an elderly female patient who developed acute and severe hepatitis with two distinct peaks following blood transfusion. The serological markers and histopathology during the first attack were characteristic for hepatitis non-A, non-B (NANBH), whereas the second peak was due to hepatitis B virus (HBV) infection. Electron microscopy revealed morphologic equivalents for both hepatitis agents.

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Ultrastructural features in chronic non‐A, non‐B (NANB) hepatitis: A controlled blind study

Cited by 14 publications

References 19 publications

Histologic Observations in Human Hepatitis Non-A, Non-B

Histologic Observations in Human Hepatitis Non-A, Non-B

Intracytoplasmic inclusions in human hepatocytes in non‐A, non‐B hepatitis: An ultrastructural study

Simultaneous infection with non‐A, non‐B and B viruses in a patient exhibiting different histologic patterns of acute hepatitis

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