Ultrastructural evidences of growth inhibitory effects of a novel biocide, Akacid®plus, on an aflatoxigenic Aspergillus parasiticus

Razzaghi-Abyaneh, Mehdi; Shams-Ghahfarokhi, Masoomeh; Kawachi, Masanobu; Eslamifar, Ali; Schmidt, Oskar J.; Schmidt, Andreas; Allameh, Abdolamir; Yoshinari, Tomoya

doi:10.1016/j.toxicon.2006.09.002

Cited by 29 publications

(22 citation statements)

References 20 publications

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“…[35] investigated the morphological alterations of A. parasiticus in the presence of neem extract and demonstrated vacuolisation of the cytoplasm, creating gaps between the plasma membrane and cell wall and local depressions in the cell wall at different intervals. Similar damages were observed in Microsporum canis treated with an imidazole compound, econazole, 36 in A. parasiticus treated with a guanidine polymer detergent, akacid, 37 and in Podosphaera fusca treated with lipopeptides from Bacillus cereus 38 . Morphological and structural changes observed in this study had a dose‐dependent trend, i.e.…”

Section: Discussionsupporting

confidence: 83%

Effect of Zataria multiflora Boiss. essential oil on colony morphology and ultrastructure of Aspergillus flavus

Gandomi

Misaghi

Basti

et al. 2010

Mycoses

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The mode of inhibitory action of Zataria multiflora Boiss. essential oil (EO) on the fungus, Aspergillus flavus, was studied by colony morphology examination, light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The EO at concentrations used in this study suppressed the size of the colony as well as sporulation. SEM of mycelia treated with given concentrations of EO showed morphological alterations ranging from loss of turgidity and uniformity of mycelia at low concentrations of EO to evident destruction of the hyphae at higher concentration of EO. Semi-thin sections of mycelia exposed to different concentrations of EO were analysed by light microscopy and revealed that the major change at level as low as 50 ppm of EO was limited to vacuolisation of cytoplasm resulting in cell swelling, while at higher concentrations, detachment of the cell membrane from the cell wall, deformation of mycelia and shedding the cytoplasm from the cell were the main alterations. These damages were well documented by TEM, which showed that the main sites of action of EO were the plasma membrane and cell wall. In conclusion, morphological and structural changes observed in this study may be one of the mechanisms involved in growth inhibition of the fungi and reducing aflatoxin production.

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Section: Discussionsupporting

confidence: 83%

Effect of Zataria multiflora Boiss. essential oil on colony morphology and ultrastructure of Aspergillus flavus

Gandomi

Misaghi

Basti

et al. 2010

Mycoses

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“…Lime essential oil was proven to stunt hypha development of A. flavus by attacking many targets, particularly membrane-bound structures, while the cell wall remained intact. These TEM observations were partially similar to morphological alteration in A. parasiticus treated with Akacid ®plus (commercial guanidine-based polymeric disinfectants) demonstrated by Razzaghi-Abyaneh et al (2006). Akacid ®plus was demonstrated to target and disrupt not only plasma membrane but also membrane of major organelle such as nuclei and mitochondria without any damage on the cell wall.…”

Section: Resultssupporting

confidence: 75%

Effects of Tropical Citrus Essential Oils on Growth, Aflatoxin Production, and Ultrastructure Alterations of Aspergillus flavus and Aspergillus parasiticus

Rammanee

Hongpattarakere

2011

Food Bioprocess Technol

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Ethyl acetate extracts and hydrodistillated essential oils from five cultivars of tropical citrus epicarps were evaluated for their inhibitory activities against Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Aspergillus parasiticus, and Penicillium sp. using disk diffusion and broth microdilution assays. Essential oils prepared from kaffir lime (Citrus hystrix DC) and acid lime (Citrus aurantifolia Swingle) epicarps exhibited stronger antifungal activity to all fungi than their ethyl acetate extracts with minimum inhibitory concentration and minimum fungicidal concentration values of 0.56 and 1.13 mg/ml (dry matter), respectively, against aflatoxin-producing A. flavus and A. parasiticus. The dominant components of the essential oil from kaffir lime were limonene, citronellol, linalool, o-cymene, and camphene, whereas limonene and p-cymene were major components of acid lime essential oil. Pure limonene, citronellal, and citronellol were five to six times less fungicidal than the natural essential oils, indicating the synergistic activity of many active compounds present in the oils. Kaffir and acid lime essential oils significantly reduced aflatoxin production of A. flavus and A. parasiticus, particularly lime essential oil, which completely inhibited growth and aflatoxin production of A. flavus at the concentration of 2.25 mg/ml. Target cell damage caused by acid lime essential oil was investigated under transmission electron microscopy. Destructive alterations of plasma and nucleus membrane, loss of cytoplasm, vacuole fusion, and detachment of fibrillar layer were clearly exhibited in essential-oil-treated cells.

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“…_ 6 ) T D $ F I G ] the cytoplasmic organelles, damaging the mitochondria (Kong and Chung, 1998;Razzaghi-Abyaneh et al, 2006;Dutot et al, 2008). Since the signals from damaged mitochondria increase ROS production on the surface of the endoplasmic reticulum (Leadsham et al, 2013), we assume that PHMG-phosphate passes through the membrane like other members of the guanidine family, generating high levels of ROS that react with mitochondria, playing an important role in the cytotoxic effect of PHMGphosphate.…”

Section: Discussionmentioning

confidence: 99%

The role of NF-κB signaling pathway in polyhexamethylene guanidine phosphate induced inflammatory response in mouse macrophage RAW264.7 cells

2015

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Ultrastructural evidences of growth inhibitory effects of a novel biocide, Akacid®plus, on an aflatoxigenic Aspergillus parasiticus

Cited by 29 publications

References 20 publications

Effect of Zataria multiflora Boiss. essential oil on colony morphology and ultrastructure of Aspergillus flavus

Effect of Zataria multiflora Boiss. essential oil on colony morphology and ultrastructure of Aspergillus flavus

Effects of Tropical Citrus Essential Oils on Growth, Aflatoxin Production, and Ultrastructure Alterations of Aspergillus flavus and Aspergillus parasiticus

The role of NF-κB signaling pathway in polyhexamethylene guanidine phosphate induced inflammatory response in mouse macrophage RAW264.7 cells

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