Ultrastructural evidence for complement and antibody-dependent damage to schistosomula of <i>Schistosoma mansoni</i> by rat eosinophils <i>in vitro</i>

McLaren, Diane J.; Ramalho-Pinto, F. J.; Smithers, S. R.

doi:10.1017/s0031182000050277

Cited by 87 publications

(32 citation statements)

References 7 publications

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“…The appearance of these blebs was reported and was suggested to be the result of the action of complement ( McLaren et al 1978 ). However, in our in vitro experimental system, there was no complement or the complement was inactivated.…”

Section: Discussionmentioning

confidence: 99%

A Microtus fortisprotein, serum albumin, is a novel inhibitor of Schistosoma japonicumschistosomula

Xiong

et al. 2013

Mem. Inst. Oswaldo Cruz

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Schistosomiasis is an endemic parasite disease and praziquantel is the only drug currently in use to control this disease. Experimental and epidemiological evidence strongly suggests that Microtus fortis ( Mf ) is a naturally resistant vertebrate host of Schistosoma japonicum . In the present study, we found that Mf serum albumin ( Mf -albumin) and the conditioned medium of pcDNA3.1- Mf -albumin caused 46.2% and 38.7% schistosomula death rates in 96 h, respectively, which were significantly higher than that of the negative control (p < 0.05). We also found that mice injected with Mf -albumin had a 43.5% reduction in worm burden and a 48.1% reduction in liver eggs per gram (p < 0.05) in comparison to the control animals. To characterise the mechanisms involved in clearance, schistosomula were incubated with fluorescein isothiocyanate-labelled Mf -albumin and fluorescent enrichment effects were found in the gut lumen of schistosomula after 48 h of incubation. Next, digestive tract excretions from schistosomula were collected and the sensitivity of Mf -albumin to digestive tract excretions was evaluated. The results indicated that schistosomula digestive tract excretions showed indigestibility of Mf -albumin. The death of schistosomula could be partially attributed to the lack of digestion of Mf -albumin by digestive tract excretions during the development of the schistosomula stage. Therefore, these data indicate the potential of Mf -albumin as one of the major selective forces for schistosomiasis.

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Section: Discussionmentioning

confidence: 99%

A Microtus fortisprotein, serum albumin, is a novel inhibitor of Schistosoma japonicumschistosomula

Xiong

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…Serial non-destructive blood sampling followed by ELISA, may be used to determine the timing infection of immature flukes invading T. maccoyii host, however the method would need to be validated first. Detection of anti-fluke antibody could be useful in addition to parasite detection and in some cases can have 92–97% sensitivity [32], yet in Schistosoma infections in humans ELISA antibody detection cannot be reliably used until three months post exposure to the parasite [33]. Alternatives, such as detection of circulating antigen may be effective for diagnosis of infection at the earliest stages.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Humoral Immune Response in Southern Bluefin Tuna, T. maccoyii, with Infection Stage of the Blood Fluke, Cardicola forsteri

et al. 2012

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The blood fluke, Cardicola forsteri, is a prevalent infection in ranched southern bluefin tuna. This project aimed to define the timing and intensity of the various developmental stages of C. forsteri within southern bluefin tuna as well as to relate infection to host pathology and immune response. Archival samples from several cohorts of T. maccoyii sampled from 2008 to 2010 were used in this study. The prevalence and intensity of C. forsteri infection was described using heart flushes and histological examination. Humoral immune response, i.e. C. forsteri specific antibody, lysozyme activity, and alternative complement activity, was also described. Based on the validated and detailed C. forsteri infection timeline, relationships between infection events, physiological response, and diagnosis were proposed. Immune response developed concurrently with C. forsteri infection, with the majority of physiological response coinciding with commencing egg production. Further research is needed to confirm the origin of C. forsteri antigen which is responsible for immune response development and how T. maccoyii immune response works against infection. To aide this research, further diagnostic methods for confirmation of infection need to be developed.

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“…Parasite opsonization with IgG, IgE and/or C3b permits eosinophil adhesion to the parasite surface and facilitates eosinophil degranulation (Butterworth et al, 1977(Butterworth et al, , 1979aMcLaren et aL, 1978;Anwar et al, 1979;Capron et al, 1981a). Antibodydependent cytotoxicity is effected through the IgG RII Fc receptor (Fanger et al, 1989) or by light-density eosinophils bearing IgE Fc receptors (Fukuda et aL, 1985).…”

Section: Eosinophils Directed Against Parasitesmentioning

confidence: 99%

Eosinophils: A review

1992

Vet Res Commun

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The eosinophil was discovered by Jones in 1846 (Dessein and David, 1982) but its proclivity to stain with aniline dyes was first described by Paul Ehrlich in 1879 (Hirsch and Hirsch, 1980). Recognized and named for this quality, eosinophils possess an abundance of highly basic proteins within their granules which confer their affinity for acidic dyes (Gleich and Loegering, 1984). Eosinophils are traditionally viewed as killer-effector cells in parasitic infestations and as modulators of Type I hypersensitivity reactions (Butterworth and David, 1981; Kay, 1985). The eosinophils' reserve of cationic proteins and enzymes which imparts their profound parasiticidal effects (Butterworth and David, 1981) contrasts with this leukocyte's purported regulatory function in inflammation (Kay, 1985; Fechter et al., 1986). The opposing functions possessed by this leukocyte exemplify the enigma of the eosinophil. Recent evidence suggests that although the eosinophil does posses some regulatory capabilities, its presence is, in fact, a harbinger of tissue destruction (Gleich and Adolphoson, 1986, Wardlaw and Kay, 1987; Spry, 1988). Nor does the presence of the eosinophil automatically infer IgE mediated hypersensitivity, as evidenced by studies examining the interaction of the eosinophil with the cellular arm of the immune system (Basten and Beeson, 1970; Ruscetti et al., 1976; Beeson and Bass, 1977; Raghavachar et al., 1987; Ohnishi et al., 1988). The purpose of this review is to provide a brief overview of the structure and biology of the mammalian eosinophil and to emphasize the fact that eosinophils fulfil a paradoxical role as effectors of tissue damage and as benign modulators of inflammation.

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Ultrastructural evidence for complement and antibody-dependent damage to schistosomula of Schistosoma mansoni by rat eosinophils in vitro

Cited by 87 publications

References 7 publications

A Microtus fortisprotein, serum albumin, is a novel inhibitor of Schistosoma japonicumschistosomula

A Microtus fortisprotein, serum albumin, is a novel inhibitor of Schistosoma japonicumschistosomula

Correlation of Humoral Immune Response in Southern Bluefin Tuna, T. maccoyii, with Infection Stage of the Blood Fluke, Cardicola forsteri

Eosinophils: A review

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