Ultrastructural Details of Cryptosporidium parvum Development in Calf Intestine

Rosales, María J.; Arnedo, Teresa; Mascaró, C.

doi:10.1590/s0074-02761998000600027

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…This may be due to the fact that it is difficult to identify PVs using optical microscopy and hence the formation of a PV in cell-free culture has gone undetected. The PVs observed here (in both biofilm systems and HCT8 cell cultures) were very similar to those observed in previous in - vitro and in - vivo studies [ 23 , 25 - 28 ], but these studies also believed that the undulated membrane was derived from the host microvillus. The formation of a PV in our host free system is consistent with the proposal by Pohlenz et al [ 29 ] that Cryptosporidium does not require host encapsulation to form a PV.…”

Section: Discussionsupporting

confidence: 89%

Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems

et al. 2014

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BackgroundAquatic biofilms often serve as environmental reservoirs for microorganisms and provide them with a nutrient-rich growth environment under harsh conditions. With regard to Cryptosporidium, biofilms can serve as environmental reservoirs for oocysts, but may also support the growth of additional Cryptosporidium stages.ResultsHere we used confocal laser scanning microscopy, scanning electron microscopy (SEM), and flow cytometry to identify and describe various Cryptosporidium developmental stages present within aquatic biofilm systems, and to directly compare these to stages produced in cell culture. We also show that Cryptosporidium has the ability to form a parasitophorous vacuole independently, in a host-free biofilm environment, potentially allowing them to complete an extracellular life cycle. Correlative data from confocal and SEM imaging of the same cells confirmed that the observed developmental stages (including trophozoites, meronts, and merozoites) were Cryptosporidium. These microscopy observations were further supported by flow cytometric analyses, where excysted oocyst populations were detected in 1, 3 and 6 day-old Cryptosporidium-exposed biofilms, but not in biofilm-free controls.ConclusionsThese observations not only highlight the risk that aquatic biofilms pose in regards to Cryptosporidium outbreaks from water distribution systems, but further indicate that even simple biofilms are able to stimulate oocyst excystation and support the extracellular multiplication and development of Cryptosporidium within aquatic environments.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-014-0281-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 89%

Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems

et al. 2014

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“…This may be due to the fact that it is difficult to identify PVs using optical microscopy and hence the formation of a PV in cell-free culture has gone undetected. The PVs observed here (in both biofilm systems and HCT8 cell cultures) were very similar to those observed in previous in-vitro and in-vivo studies [23,[25][26][27][28], but these studies also believed that the undulated membrane was derived from the host microvillus. The formation of a PV in our host free system is consistent with the proposal by Pohlenz et al [29] that Cryptosporidium does not require host encapsulation to form a PV.…”

Section: Discussionsupporting

confidence: 88%

Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems

Koh¹,

Thompson²,

Edwards³

et al. 2021

Preprint

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Background Aquatic biofilms often serve as environmental reservoirs for microorganisms and provide them with a nutrient-rich growth environment under harsh conditions. With regard to Cryptosporidium, biofilms can serve as environmental reservoirs for oocysts, but may also support the growth of additional Cryptosporidium stages. Results Here we used confocal laser scanning microscopy, scanning electron microscopy (SEM), and flow cytometry to identify and describe various Cryptosporidium developmental stages present within aquatic biofilm systems, and to directly compare these to stages produced in cell culture. We also show that Cryptosporidium has the ability to form a parasitophorous vacuole independently, in a host-free biofilm environment, potentially allowing them to complete an extracellular life cycle. Correlative data from confocal and SEM imaging of the same cells confirmed that the observed developmental stages (including trophozoites, meronts, and merozoites) were Cryptosporidium. These microscopy observations were further supported by flow cytometric analyses, where excysted oocyst populations were detected in 1, 3 and 6 day-old Cryptosporidium-exposed biofilms, but not in biofilm-free controls. Conclusions These observations not only highlight the risk that aquatic biofilms pose in regards to Cryptosporidium outbreaks from water distribution systems, but further indicate that even simple biofilms are able to stimulate oocyst excystation and support the extracellular multiplication and development of Cryptosporidium within aquatic environments.

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“…in 100% of animals along the entire intestinal tract. The observed intensive loss of intestinal villi, especially in the distal small intestinal compartment, is associated exactly with established extensive Cryptosporidium in fection (Rosales et al, 1998). At the same time, we believe that the observed signs of regeneration and repair, mainly under the form of crypt hyperplasia in about one quarter of cases (5 calves) are an attempt for restoration of mucosal cell barrier, although at an insufficient extent.…”

mentioning

confidence: 68%

Clinical and morphological investigations in a spontaneous cryptosporidium enteritis outbreak in calves

Kalkanov¹,

Dinev²,

Dimitrov³

et al. 2016

BJVM

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SummaryKalkanov, I., I. Dinev, K. Dimitrov & P. Iliev, 2016. Clinical and morphological investigations in a spontaneous Cryptosporidium enteritis outbreak in calves. Bulg. J. Vet. Med., 19, No 4,[334][335][336][337][338][339] The purpose of this report was to present the results of histopathological examination during the course of a natural Cryptosporidium parvum outbreak, as well as the morphology of cells infiltrating affected gastrointestinal tract areas in newborn calves. The study included 18 calves exhibiting a marked diarrhoeic syndrome, between 1 and 8 days of age. Sporulated C. parvum oocysts were demonstrated in faecal smears stained by the method of Henriksen. Coproantigens of C. parvum were detected by the rapid Rainbow calf scour 5 BIO K 306 test. Gross lesions were mainly present in the gastrointestinal tract, together with inflammation in regional mesenteric lymph nodes. Microscopic histopathological lesions consisted mainly in desquamative catarrh of intestinal mucosa and intestinal villous atrophy. The affected intestinal epithelium contained multiple Cryptosporidium spp. forms at different stage of the life cycle. The results from histopathological studies of the outbreak allowed confirming some main morphogenetic features of the disease caused by C. parvum in newborn and growing calves.

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Ultrastructural Details of Cryptosporidium parvum Development in Calf Intestine

Cited by 6 publications

References 17 publications

Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems

Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems

Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems

Clinical and morphological investigations in a spontaneous cryptosporidium enteritis outbreak in calves

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