Ultrastructural cytochemistry of the nucleus and nucleolus in growing rabbit oocytes

Šutovský, Peter; Jelínková, L; Antalíková, L; Motlı́k, Jan

doi:10.1016/s0248-4900(05)80185-6

Cited by 14 publications

(6 citation statements)

References 38 publications

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“…The ultrastructural analysis showed that the agouti oocyte presented a central and regular nuclei, displaying a homogenous mass, similar to the features described for mice (Chouinard 1971), but different from hamster (Takeuchi et al 1984) and rat (Antoine et al 1987), in which the oocyte nucleoli become a compact homogenous mass at the antral follicle stage (Sutovskỳ et al 1993). It was previously reported that nucleolar compaction during oogenesis does not occur at the same stage in all species and those differences are correlated with a decrease in RNA synthesis along the follicular development (Antoine et al 1989).…”

Section: Discussionsupporting

confidence: 74%

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

et al. 2018

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The aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 14.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyte Golgi apparatus was rarely observed. In conclusion, this work shows for the first time the characterization of the population of preantral follicles in the ovary of Dasyprocta leporina. Those information will be useful for further development and adaptation of biotechniques such as germplasm cryopreservation and in vitro gametes manipulation.

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Section: Discussionsupporting

confidence: 74%

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

et al. 2018

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“…Hall & Levison, 1990;Derenzini & Ploton, 1991;Plate et al, 1992;Rathwolfson et al, 1995), cell biology (e.g. Ploton et aI., 1987;Sutovsky et al, 1993) and developmental biology (Boloukhere, 1984;Gaginskaya et al, 1988;Standiford, 1988;Rue & Bierne, 1988;Biliriski, 1989). Several modifica-BILINSKI and BILII~ISKA tions of the technique for various biological applications have recently been suggested (e.g.…”

Section: Introductionmentioning

confidence: 99%

A new version of the Ag−NOR technique. A combination with DAPI staining

Biliński

Bilińska

1996

Histochem J

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The Ag-NOR staining technique is widely used for visualizing nucleolar organizer regions (NORs) in various plant and animal tissues. We describe a simple and time-saving combination of Ag-NOR staining with DNA detection by fluorescence microscopy. This modification was tested on cultured cells and semi-thin sections of plastic-embedded tissues. Of the different fixatives and embedding media used in our studies, the best results (i.e., high selectivity of staining, and lack of or very low background precipitation) were obtained with fixation in methanol-acetone at-20 degrees C for cultured cells, and fixation in 4% formaldehyde followed by embedding in Histocryl resin for tissue sections. The optimal time of Ag-NOR staining was determined experimentally for all materials tested. The specificity of the staining was checked at the electron microscopical level. Especially good results were obtained by mixing epifluorescence with standard bright-field illumination. In such a combination, Ag-NOR-positive nucleoli, or their fibrillar centres and dense fibrillar components, were clearly visible against a bright background of nuclear DNA.

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“…However, during folliculogenesis, the molecular machinery of the oocyte is active. The nucleolus has a structure that reflects fibrogranular synthetic activity of the oocyte during growth (Sutovsky et al, 1993). During this period, the ovary synthesizes and stores large amounts of messenger ribonucleic acid (mRNA) and protein increasing their size (Motlik et al, 1989).…”

Section: Oogenesis and Follicular Developmentmentioning

confidence: 99%

“…This phase is characterized by the presence of a configuration prominent nuclear called germinal vesicle (GV) (Sutovsky et al, 1993). The peak preovulatory LH triggers the resumption of meiosis due to loss of unions "gap" between the cumulus and the oocyte, as a result of the expansion of the former.…”

Section: Nuclear Maturationmentioning

confidence: 99%

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Biostimulation methods associated with early weaning and reproductive rhythms in primiparous rabbit does

Sakr¹

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Ultrastructural cytochemistry of the nucleus and nucleolus in growing rabbit oocytes

Cited by 14 publications

References 38 publications

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

A new version of the Ag−NOR technique. A combination with DAPI staining

Biostimulation methods associated with early weaning and reproductive rhythms in primiparous rabbit does

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