Ultrastructural Characterization of Germ Cell Apoptosis in Goat (&lt;I&gt;Capra hircus&lt;/I&gt;) Testes

Sharma, Rakesh; Sharma, Virender Kumar; Bhardwaj, Jitender Kumar

doi:10.1166/jamr.2011.1077

Cited by 6 publications

(4 citation statements)

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“…In addition, Choudhary et al . revealed that malathion also altered the spermatogenesis leading to infertility troubles by affecting the male reproductive system of an organism . It has been reported that malathion alters the proliferation of the seminiferous epithelium by impairing steroidogenesis, and that causes death in germ cells .…”

Section: Introductionmentioning

confidence: 99%

N‐Acetyl‐cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue

Bhardwaj

Saraf

Kumari

et al. 2018

J Biochem & Molecular Tox

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Toxicological studies so far suggest that excessive use of malathion, an organophosphate insecticide, causes serious ill-effects in mammalian reproductive physiology. The present study aims at assessing malathion-induced toxicity in a dose- and time-dependent manner with mitigating effects of N-acetyl-l-cysteine. The testicular germ cell viability was monitored using MTT assay, where NAC, being an antioxidant significantly reduced malathion-induced toxicity by enhancing the frequency of cell viability. The histomorphological analysis showed that NAC successfully diminished several apoptotic features in testicular cells, induced by malathion. The differential EB/AO staining revealed a significant decline in the percentage of apoptosis after NAC supplementation. NAC also diminished the malathion-induced DNA fragmentation along with significantly reduction in oxidative stress parameters causing decrease in lipid peroxidation and enhancement of ferric reducing antioxidant power within testicular germ cells. Thus, NAC mitigated the malathion-induced toxicity, proving its potential in infertility treatment.

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Section: Introductionmentioning

confidence: 99%

N‐Acetyl‐cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue

Bhardwaj

Saraf

Kumari

et al. 2018

J Biochem & Molecular Tox

View full text Add to dashboard Cite

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“…After treatment, the testicular tissue/cells were examined for various parameters such as histology, fluorescence, and biochemical test. [ 25 ]…”

Section: Methodsmentioning

confidence: 99%

Efficacy of N‐acetyl‐l‐cysteine against glyphosate induced oxidative stress and apoptosis in testicular germ cells preventing infertility

Bhardwaj

Kumar

Saraf

et al. 2021

J Biochem & Molecular Tox

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The present era's demand for continuous pesticides' use to increase the agriculture outcome, has caused numerous health effects among which mammalian infertility, owing to reproductive toxicity, is serious. Thus, the present study emphasizes upon glyphosate (GLY) induced toxicity and mitigating effects of N‐acetyl cysteine (NAC) in testicular cells of caprine by using various cytotoxic and biochemical parameters. GLY was found to induce several apoptotic attributes such as pyknotic nuclei, tubular degeneration, increased vacuolization, and so on, in testicular cells. GLY also decreased the cell viability and increased the incidence of apoptosis in testicular cells in a dose‐ and time‐dependent manner as revealed by MTT assay and Fluorescence (ethidium bromide/acridine orange) assay, respectively. It also increased the level of oxidative stress as evident with an increase in lipid peroxidation and decline in antioxidant power along with the decreased enzymatic activity of different antioxidant enzymes (SOD, CAT, and GST). However, NAC supplementation showed antagonistic results in GLY‐treated testicular tissues with maximum amelioration at the highest dose, thereby decreasing GLY‐mediated apoptosis rate and oxidative stress. Maximum amelioration was reported at 10 mM NAC concentration. Reduced GLY toxicity due to NAC will prove NAC to be an excellent approach for dealing with male reproductive toxicity at the cellular level, benefiting the mammalian reproductive status.

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“…Therefore, it is more sensitive to changes in structure of the glass network as compared to mean atomic volume. It is also associated with the free volume and flexibility of this network [53]. It was calculated from the relation [54]:…”

Section: Deduced Bymentioning

confidence: 99%

Analysis of glass forming ability using percolation concept and tunability of physical parameters of a-Ge₁₂Se₇₆-_xAs₁₂Bi_xglassy semiconductors

Sharma

Sunder

2018

Materials Science-Poland

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Glass forming ability of lone-pair semiconductors was analyzed for (x = 0, 2, 4, 6, 8, 10) system. Values of lone pair electrons L were calculated using average coordination number of valence electrons. These values were found to decrease, as the system was moving towards the rigid region. L > 3 values showed vitreous state. Deviation of the stoichiometry confirmed the chalcogen-rich region. A linear correlation was found between the mean bond energy and glass transition temperature. Chemical Bond Approach model was applied to calculate the cohesive energy of the system. A linear relationship was found to exist between the cohesive energy and the theoretical band gap, calculated using Shimakawa relation. A decrease in both parameters was explained on the basis of average stabilization energy and electronegativity of the system. The density values were found to increase and may account for higher refractive index of the system. Large Bohr radius of the Bi atom accounted for an increase in the polarizability. Other parameters viz. degree of covalency, packing density, compactness, molar volume, free volume percentage, excess volume and polaron radius were also calculated. An effort was made to correlate the effect of Bi addition to Ge12Se76 - xAs12Bixlone-pair semiconductor on the basis of the structure of the glassy matrix or the connectedness of the material.

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Ultrastructural Characterization of Germ Cell Apoptosis in Goat (<I>Capra hircus</I>) Testes

Cited by 6 publications

References 0 publications

N‐Acetyl‐cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue

N‐Acetyl‐cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue

Efficacy of N‐acetyl‐l‐cysteine against glyphosate induced oxidative stress and apoptosis in testicular germ cells preventing infertility

Analysis of glass forming ability using percolation concept and tunability of physical parameters of a-Ge₁₂Se₇₆-_xAs₁₂Bi_xglassy semiconductors

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Ultrastructural Characterization of Germ Cell Apoptosis in Goat (<I>Capra hircus</I>) Testes

Cited by 6 publications

References 0 publications

N‐Acetyl‐cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue

N‐Acetyl‐cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue

Efficacy of N‐acetyl‐l‐cysteine against glyphosate induced oxidative stress and apoptosis in testicular germ cells preventing infertility

Analysis of glass forming ability using percolation concept and tunability of physical parameters of a-Ge12Se76-xAs12Bixglassy semiconductors

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Analysis of glass forming ability using percolation concept and tunability of physical parameters of a-Ge₁₂Se₇₆-_xAs₁₂Bi_xglassy semiconductors