Ultrastructural characteristics of rat peritoneal mast cells undergoing differential release of serotonin without histamine and without degranulation

Kops, Sandra Kraeuter; Theoharides, Theoharis C.; Cronin, Claire; Kashgarian, Michaele; Askenase, P. W.

doi:10.1007/bf00305238

Cited by 64 publications

(28 citation statements)

References 23 publications

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“…However, this differential release of serotonin involved vesicles containing high affinity serotonin binding proteins (45) that appeared to bud off from secretory granules (46). Similarly, mast cells were recently reported to transport fibroblast growth factor in vesicles, but these vesicles also derived from secretory granules (47).…”

Section: Discussionmentioning

confidence: 99%

IL-1 Induces Vesicular Secretion of IL-6 without Degranulation from Human Mast Cells

Kandere‐Grzybowska

Létourneau

Kempuraj

et al. 2003

The Journal of Immunology

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213

144

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FcεRI cross-linkage in mast cells results in release of granule-associated mediators, such as histamine and proteases, as well as the production of numerous cytokines, including IL-6. Mast cells have been increasingly implicated in inflammatory processes where explosive degranulation is not commonly observed. Here, we show that IL-1 stimulates secretion of IL-6 without release of the granule-associated protease tryptase in normal human umbilical cord blood-derived mast cells (hCBMCs). IL-6 secretion stimulated by IL-1 in hCBMCs is potentiated by priming with IL-4 and reflects the higher levels of IL-6 secreted from human leukemic mast cell line (HMC-1). Stimulating HMC-1 cells by both IL-1 and TNF-α results in synergistic secretion of IL-6. IL-6 is de novo synthesized, as its secretion is blocked by inhibitors of transcription or protein synthesis. IL-1 does not increase intracellular calcium ion levels in either hCBMCs or HMC-1 cells, and IL-6 stimulation proceeds in the absence of extracellular calcium ions. Ultrastructural Immunogold localization shows that IL-6 is excluded from the secretory granules and is compartmentalized in 40- to 80-nm vesicular structures. Selective secretion of IL-6 from mast cells appears distinct from degranulation and may contribute to the development of inflammation, where the importance of IL-6 has been recognized.

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Section: Discussionmentioning

confidence: 99%

IL-1 Induces Vesicular Secretion of IL-6 without Degranulation from Human Mast Cells

Kandere‐Grzybowska

Létourneau

Kempuraj

et al. 2003

The Journal of Immunology

Self Cite

213

144

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“…He was able to catch synaptic vesicles connected through a narrow neck to the synaptic cleft, taking up HRP through the fusion pore. Vesicles were seen in association with the plasma membrane and, in rare instances, these vesicles were captured in the process of forming an 'omega' shape as they were fusing with the plasma membrane (Fernández-Peruchena et al 2005;Holt et al 2004;Kops et al 1990;Sesaki & Ogihara, 1997). More interestingly, some synaptic vesicles were already labeled inside the presynaptic terminal.…”

Section: An Overview Of Exocytosis and Endocytosismentioning

confidence: 99%

The evidence for open and closed exocytosis as the primary release mechanism

Ren

Mellander

Keighron

et al. 2016

Quart. Rev. Biophys.

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Abstract. Exocytosis is the fundamental process by which cells communicate with each other. The events that lead up to the fusion of a vesicle loaded with chemical messenger with the cell membrane were the subject of a Nobel Prize in 2013. However, the processes occurring after the initial formation of a fusion pore are very much still in debate. The release of chemical messenger has traditionally been thought to occur through full distention of the vesicle membrane, hence assuming exocytosis to be all or none. In contrast to the all or none hypothesis, here we discuss the evidence that during exocytosis the vesicle-membrane pore opens to release only a portion of the transmitter content during exocytosis and then close again. This open and closed exocytosis is distinct from kiss-and-run exocytosis, in that it appears to be the main content released during regular exocytosis. The evidence for this partial release via open and closed exocytosis is presented considering primarily the quantitative evidence obtained with amperometry.1. An overview of exocytosis and endocytosis 2 2. Techniques to monitor exocytosis 5 2.1. Patch clamp 6 2.2. Amperometry 6 2.3. Patch amperometry 7 2.4. Cyclic voltammetry 7 2.5. Fluorescence microscopy imaging 83. Traditional models of exocytosis 94. Evidence where partial release during exocytosis appears to be dominant 11 4.1. The total content of a secretory vesicle is greater than the amount released 11 4.2. The majority of exocytotic events are followed by immediate endocytosis 12 4.3. Full distention of the vesicle may not be necessary for quantal release 13 4.4. A flickering fusion pore might regulate quantal release and vesicle recycling 15 4.5. Nanometer-sized pores can be seen as 'feet' associated with amperometric spikes 17 3. Release appears to be only a fraction of vesicle content in mammalian brains 23 7.4. Impact cytometry of adrenal cell vesicles shows a significantly larger catecholamine content then that released 23 7.5. Impact cytometry has been used to measure vesicle content in live cells and this quantity is greater than the amount released 23 7.6. Full distention of the vesicle is not necessary for the measured amperometric current during exocytotic release 23 7.7. Fast changes in cell environment alter the amount of messenger released 23 7.8. Patch amperometry measurements reveal a greater amount of release 23 7.9. Postspike 'feet' are observed with an amperometric spike 23Acknowledgements 24

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“…In these glands, monoaminoxidase, the enzyme that metabolizes 5-HT, is also detected (Aguilar et al, 1995;Campos et al, 1990;Ellis et al, 1972;Tinajero et al, 1993;Sanders-Bush & Mayer, 2001;Verbeuren, 1989). In rats, the testicular 5-HT can originate from the nerve endings in the capsule, the mast cells and the Leydig cells (Campos et al;Kops et al, 1990;Tinajero et al). In hamsters, the periods of sexual development and the transition between testicular regression and recrudescence are related to changes in the 5-HT levels in the mast cells and Leydig cells (Frungieri et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Neonatal Administration of Fluoxetine Decreased Final Sertoli Cell Number in Wistar Rats

Silva

Amorim

et al. 2008

Int. J. Morphol.

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SUMMARY:The aim of the present study was to test the hypothesis that the application of fluoxetine -a highly selective serotonin reuptake inhibitor (SSRI) -in rats during the suckling period induces changes in testicular development. Groups of newborn male rats were randomly assigned with different doses of fluoxetine 24 hours after birth. Each litter stayed with its respective mother during 21 days. Body weight (BW) was measured daily from the 1st -21 st day to calculate daily doses of fluoxetine. 5 mg (T1), 10 mg (T2) 20 mg (T3) or deionized water, were injected intraperitoneally. On the 21 st day, animals were heparinized, anesthetized and blood was collected by cardiac puncture to determine by radioimmunoassay the follicle stimulating hormone (FSH) levels. Testis were removed, weighed, and processed for morphometric analysis. Fluoxetine groups presented decreased body and testicular weight when compared with the control group on the 21 st day. Our findings show that the manipulation of the serotoninergic system with fluoxetine during the critical period of testicular development alters the Sertoli cell population and all testicular parameters related to this cell.

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Ultrastructural characteristics of rat peritoneal mast cells undergoing differential release of serotonin without histamine and without degranulation

Cited by 64 publications

References 23 publications

IL-1 Induces Vesicular Secretion of IL-6 without Degranulation from Human Mast Cells

IL-1 Induces Vesicular Secretion of IL-6 without Degranulation from Human Mast Cells

The evidence for open and closed exocytosis as the primary release mechanism

Neonatal Administration of Fluoxetine Decreased Final Sertoli Cell Number in Wistar Rats

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