Ultrastructural Characteristics of Human Tumor Cells in Vitro

Seman, G; Dmochowski, L

doi:10.1007/978-1-4757-1647-4_14

Cited by 9 publications

(4 citation statements)

References 190 publications

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“…4) (Kumegawa et al, 1968) and annulate lamellae (Kumegawa et al, 1967). Cytoplasmic fractures (Seman and Dmochowski, 1975) were evident in many J82 cells. Their significance is unknown.…”

Section: Micromorphologymentioning

confidence: 99%

Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

O’Toole¹,

Price²,

Ohnuki³

et al. 1978

Br J Cancer

138

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Summary.-A cell line (J82) was derived from a poorly differentiated, invasive, transitional-cell carcinoma, Stage T3. The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology.The majority of cells contained 2Y chromosomes and several distinctive markers. Peripheral-blood lymphocytes from the donor of the J82 cells were tested sequentially for cytotoxicity toward autologous and allogeneic tumour cells. Autologous cyto Foxicity was detected against J82 cells in early in vitro passage. Allogeneic lymphocytes from some patients with transitional-cell carcinoma were also cytotoxic to J82 cells in primary culture. However, selective cytotoxicity by lymphoid cells from bladdercarcinoma patients was not detected against J82 cells in long-term tissue culture.

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“…4) (Kumegawa et al, 1968) and annulate lamellae (Kumegawa et al, 1967). Cytoplasmic fractures (Seman and Dmochowski, 1975) were evident in many J82 cells. Their significance is unknown.…”

Section: Micromorphologymentioning

confidence: 99%

Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

O’Toole¹,

Price²,

Ohnuki³

et al. 1978

Br J Cancer

138

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“…Electron microscopic examination of cultured cardiac myocytes treated with EGTA (see above) shows the same phenomenon as described for cultured epithelial cells, namely the appearance of numerous cytoplasmic vesicles associated with a 'cap' of dense plaque material and tufts of IF (Figure 4a -e; for epithelial cells, see also Kartenbeck et al, 1982;cf., Overton, 1968;Seman and Dmochowski, 1975). Frequently, one IF bundle can be associated with two or more of these vesicles ( Figure 4a).…”

Section: Introductionmentioning

confidence: 99%

Specific attachment of desmin filaments to desmosomal plaques in cardiac myocytes.

Kartenbeck¹,

Franke²,

Moser³

et al. 1983

The EMBO Journal

117

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“…Ultrastructural examination showed the presence of maculae adherentes, confirming the epithelial nature of the tumor cells (Fig. I) [15]. No type C virus particles were observed.…”

Section: Methodsmentioning

confidence: 83%

Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody

Capone

Kadohama

Chu

1987

Cancer Immunol Immunother

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A new spontaneously arisen murine breast tumor, designated JC, has been established in immunocompetent BALB/c mice. Upon reestablishment of tumor in vitro and in vivo, the epithelial murine tumor cells retained their original papillary adenocarcinoma morphology. Various immunotherapy protocols have been performed in previously implanted and progressively growing JC tumor in syngeneic hosts with a murine monoclonal antibody (McAb), F36/22 (IgG3). Affinity of McAb binding to JC tumor cells was determined to be 6.1 x 10(7) L/M. Quantitatively 1.2 x 10(5) molecules of McAb bound to a JC tumor cell. Immunotherapeutic effectiveness in vivo on a tumor mass after its establishment is a major feature of this experimental tumor model. When four sequential administration of McAb, i.p., at a dose of 400 micrograms 4 days apart were used, McAb-treated animals showed statistically significant tumor regression and longer survival than those of control animals treated with an irrelevant McAb of the same isotype. Two temporal phases of tumoricidal activity were observed as measured by tumor volume reduction. The first phase of tumoricidal response (tumor regression) was detected within days upon the first administration of McAb. A distinct second phase followed within 3-5 weeks after the last McAb administration, which resulted in tumor necrosis even in large tumors. Histological examinations revealed heavy infiltration of inflammatory cells at the beginning of the second phase. Similar tumor regression was also obtained from animals treated with a single dose (400 micrograms) of McAb followed by injections of McAb with complete and incomplete adjuvant, respectively. These results demonstrate that this syngeneic murine mammary tumor can serve as a potential preclinical model for investigation of parameters and mechanisms associated with McAb immunotherapy.

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Ultrastructural Characteristics of Human Tumor Cells in Vitro

Cited by 9 publications

References 190 publications

Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

Specific attachment of desmin filaments to desmosomal plaques in cardiac myocytes.

Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody

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