Ultrastructural changes in the yeastCandida lipolytica caused by penetration of hydrocarbons into the cell

Ludvı́k, Jiřı́; Munk, V.; Dostálek, Milan

doi:10.1007/bf02138754

Cited by 47 publications

(22 citation statements)

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“…Occasionally, the vesicles appeared within the cytoplasm at the periphery of the cell, suggesting an endocytotic type of mechanism for hydrocarbon transport. This has been suggested by Ludvik et al (1968), who observed pinocytotic vesicles at the ends of deep invaginations of the cytoplasmic membrane in hexadecane-grown cells of C. f@oZytica. The vesicles observed in C. tropicalis resulted from growth in the presence of tetradecane vapours, indicating that direct contact of the Cellular-associated hydrocarbon inclusions 349 Ultrastructural modifications and the sequestering of hydrocarbon, resulting from the growth on hydrocarbon substrates, appear to be generalized phenomena characteristic of a variety of bacteria and the yeast Candida.…”

Section: Discussionsupporting

confidence: 57%

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A Comparative Analysis of the Ultrastructure of Hydrocarbon-oxidizing Micro-organisms

Scott

Finnerty

1976

Journal of General Microbiology

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The ultrastructure of a variety of micro-organisms was compared after growth on hydrocarbon and non-hydrocarbon substrates. Hydrocarbon-grown organisms were characterized by the presence of intracellular efectron-transparent inclusions which in many cases appeared membrane-bound. With one exception, nonhydrocarbon-grown organisms did not contain electron-transparent inclusions. Insignificant amounts of poly-P-hydroxybutyric acid were produced by the hydrocarbon-grown micro-organisms. After growth on hydrocarbons, all the microorganisms had accumulated varying amounts of the respective unmodified hydrocarbon growth substrate.

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Section: Discussionsupporting

confidence: 57%

“…The fine structure of C. lipolytica has been described after growth on hydrocarbon and non-hydrocarbon substrates (Munk, Dostalek & Volfova, I 969; Ludvik, Munk & Dostalek, 1968). Hexadecane-grown cells were said to contain a larger number of fat vacuoles, which were not positively identified.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Analysis of the Ultrastructure of Hydrocarbon-oxidizing Micro-organisms

Scott

Finnerty

1976

Journal of General Microbiology

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“…These differences may be important in conferring the structural and functional properties on the cell membranes necessary to assimilate the lipophilic hydrocarbon substrates. It is noteworthy, in this respect, that electron micrographs of yeast cells utilizing hydrocarbons have shown extensive invaginations of the plasma membrane (Ludvik, Munk & Dostalek, 1968). The mitochondrial membrane-bound enzymes (NADH oxidase, succinate oxidase and succinate dehydrogenase) of C. lipolytica cells grown on ethanol showed significantly higher specific activities and lower transition temperatures when compared with the corresponding enzymes of S. cerevisiae (Table 3).…”

Section: Discussionmentioning

confidence: 98%

A Comparative Study of Cells and Mitochondria of Saccharomyces cerevisiae and of a Hydrocarbon-utilizing Yeast, Candida lipolytica

Skipton

Watson

Houghton

et al. 1974

Journal of General Microbiology

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The properties of cells and mitochondria isolated from Candida lipolytica, a hydrocarbon-utilizing yeast, grown on ethanol, glucose and n-alkanes were examined. A comparative study was made of the properties of the whole cells and mitochondria of C. ZipoZytica and of those of Saccharomyces cerevisiae.The reduced-minus-oxidized cytochrome spectrum of C. lipolytica grown on ethanol showed a much larger amount of cytochromes aa3 and a very broad cytochrome b-type absorption band compared with S. cerevisiae. The relative amounts of cytochromes in C . lipolytica on hydrocarbons differed according to the growth phase but never reached the levels observed in the cells grown on ethanol. Furthermore, as judged by the reduced-minus-oxidized cytochrome spectra, C. lipolytica was much less affected by glucose repression than was S. cerevisiae. The fatty acids of mitochondria isolated from ethanol-grown C. l@oZytica consisted mainly of equal amounts of oleic (CI8J and linoleic (C1,:2) unsaturated fatty acids, each making up about 40 % of the total. In contrast, S. cerevisiae mitochondria contained palmitoleic (ClGZl) (approx. 45 %) and oleic (approx. 35 %) as the main fatty-acid components. There was an increase in the amount of oleic acid (60 %) relative to linoleic acid (20 %) when C. lipolytica was grown on even-numbered hydrocarbons and a progressive increase in the amount of heptadecenoic acid (C1,:,) up to 75 % when grown on odd-numbered n-alkanes of increasing carbonchain length from C,, to CI5. The changes in fatty-acid composition were correlated with changes in membrane fluidity as measured by differences in transition temperatures in Arrhenius plots of mitochondria1 membrane-bound enzymes.The ATPases of C. lipolytica and S. cerevisiae mitochondria were examined, and marked differences in specific activity (3-to 5-fold higher in S. cerevisiae), pH profile and oligomycin sensitivity were noted.

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“…They furthermore observed the formation of submicronsized droplets and assumed the direct uptake of these droplets by the yeast. A pinocytotic mechanism was also postulated for the transport of hydrocarbons in Candida lipolytica [84]. Transmission electron microscopy showed the accumulation of hexadecane and gas oil at the cell membrane and the release of pinocytotic vesicles from the cell membrane into the cytoplasm.…”

Section: Liquid Substratesmentioning

confidence: 97%

Mobilisation of Organic Compounds and Iron by Microorganisms

Harms

Wick

2004

Physicochemical Kinetics and Transport at Biointerfaces

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Ultrastructural changes in the yeastCandida lipolytica caused by penetration of hydrocarbons into the cell

Cited by 47 publications

References 4 publications

A Comparative Analysis of the Ultrastructure of Hydrocarbon-oxidizing Micro-organisms

A Comparative Analysis of the Ultrastructure of Hydrocarbon-oxidizing Micro-organisms

A Comparative Study of Cells and Mitochondria of Saccharomyces cerevisiae and of a Hydrocarbon-utilizing Yeast, Candida lipolytica

Mobilisation of Organic Compounds and Iron by Microorganisms

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