Ultrastructural changes in Paneth cells during hibernation in the ground squirrel Spermophilm lateralis

Abstract

The ultrastructure of Paneth cells from jejuno-ileal segments of the small intestine of the ground squirrel, S. lateralis, was examined under normal euthermic conditions and during the profoundly depressed metabolic conditions of natural hibernation. Paneth cells obtained from hibernating animals gave evidence of markedly reduced activity when compared to Paneth cells from euthermic animals. In hibernating animals, the nuclei were smaller, with less prominent nucleoli and with an increased proportion of heterochromatin, In hibernating animals, the rough endoplasmic reticulum was fragmentary and poorly organized, in contrast to the typical arrangement of concentric lamellae seen in euthermic animals. Although the total number of ribosomes was decreased in hibernating animals, there were proportionally more free ribosomes than in euthermic animals. Paneth cells from hibernating animals also contained a greater number of apical secretory granules which were smaller and more variable in electron density than granules from control animals. These ultrastructural features indicate that during hibernation the Paneth cell is relatively quiescent.

Ultrastructure of paneth cells in germ-free rats, with special reference to the secretory granules and lysosomes.

“…Secretory granules of variable electron density were found in the Paneth cells of squirrels during hibernation, when bacterial activity seems to be reduced due to low temperature (TOTH, 1980). In the present study, the changes in the secretory granules (i.e., membranous structures, coarse and low electron dense matrix and clear peripheral space) were observed in the germ-free rats.…”

Paneth cells of the eastern gray squirrel (Sciurus carolinensis) and red-cheeked suslik (Citellus erythrogenys brandt)

“…The extent of its development is depended on the level of cellular secretory activity. The electron-dense material of the granule is of glycoprotein nature [4,10]. In longitudinal sections of Paneth cells as many as 5-6 groups of cisternae of the Golgi apparatus are found in susliks, while in squirrels this organelle is not so numerous.…”

“…They offer advantages over the usual scalpels due to their hemostatic, aseptic, and other properties, and hence have made it possible to improve or even change the techniques of surgical treatment [2,5,7,8,10,11]. They offer advantages over the usual scalpels due to their hemostatic, aseptic, and other properties, and hence have made it possible to improve or even change the techniques of surgical treatment [2,5,7,8,10,11].…”

“…In spite of numerous data on Paneth cells, one of the most interesting populations of enterocytes of the small intestine, [7][8][9][10][11], some aspects of their functioning remain unclear. It is known that granules of these cells contain heavy metals, and digestive and lysosomal enzymes; the cells can phagocytize bacteria and release antibacterial factor [1].…”

Characterization of paneth cells in alpacas (Vicugna pacos, Mammalia, Camelidae)

“…http://dx.doi.org/10.1016/j.tice.2016.04.003 0040-8166/© 2016 Elsevier Ltd. All rights reserved. Paneth cells were identified in different species, including human being (Ehrmann et al, 1990;Erlandesen et al, 1974), rat (Rodning et al, 1982;Sundstrom and Heleander, 1980;Behnke and Moe, 1964), mouse (Satoh et al, 1992), guinea pig (Vásquez et al, 2014;Satoh et al, 1990), squirrel (Toth, 1980), hamster, monkey (Satoh et al, 1990), rabbit (Zanuzzi et al, 2008;Abdel-Magied and Taha, 1995), horse (Takehana et al, 1998), sheep (Ergün et al, 2003) and camel (Abdel-Magied and Taha, 1995). In most of the species studied, they are located at the bottom of the Lieberkhün glands of the small intestine where they stay for 18-21 days and then they are renewed .…”