Ultrastructural changes in Chlamydomonas acidophila (Chlorophyta) induced by heavy metals and polyphosphate metabolism

Nishikawa, Kenichiro; Yamakoshi, Yoko; Uemura, Isao; Tominaga, Noriko

doi:10.1016/s0168-6496(03)00049-7

Cited by 142 publications

(76 citation statements)

References 17 publications

(19 reference statements)

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“…Also, the appearance of starch in the chloroplasts might depend on the drastically inhibited growth, which would fall down the nutritional requirement of the cells. Similar changes, such as cell enlargement, cytoplasm vesiculation and starch accumulation, were also noticed in cells of Chlorella (Wong et al 1994) and Chlamydomonas acidophila (Nishikawa et al 2003) when exposed to the same heavy metal. Moreover, in K. antarctica cultures treated with 5 ppm Cd, the remains of deeply damaged and dismantled cells were present.…”

Section: Discussionsupporting

confidence: 64%

Responses of the Antarctic microalga Koliella antarctica (Trebouxiophyceae, Chlorophyta) to cadmium contamination

Rocca¹,

Andreoli²,

Giacometti³

et al. 2009

Photosynt.

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Ultrastructural and physiological effects of exposure to 1 ppm and 5 ppm of cadmium (Cd) on cultured cells of Koliella antarctica, a green microalga from Antarctica, were investigated. The amount of Cd in the alga rose with the increase of the metal concentration in the growth medium and most Cd remained outside the cells, bound to the components of the cell walls. The increase of Cd in the microalga was concomitant with the decrease of other elements, mainly calcium (Ca). Exposure to 1 ppm Cd slowed culture growth by inhibiting cell division and also caused the development of some misshapen cells with chloroplast showing disordered thylakoids. However, this concentration did not substantially affect the chlorophyll (Chl) content or photosystem (PS) activity. At 5 ppm, Cd cell growth suddenly stopped and some cells lysed. After a week of Cd contamination, the cells were enlarged and severely damaged. The chloroplasts showed great ultrastructural alterations and a reduced Chl content. Cd exposure negatively affected PSII, whose activity was almost completely lost after four days.

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Section: Discussionsupporting

confidence: 64%

Responses of the Antarctic microalga Koliella antarctica (Trebouxiophyceae, Chlorophyta) to cadmium contamination

Rocca¹,

Andreoli²,

Giacometti³

et al. 2009

Photosynt.

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“…Zn phosphate deposition has already been described in root or leaf cells of Thlapsi caerulescens (Vazquez et al, 1994;Zhao et al, 1998). Moreover, Cd phosphate was observed in the vacuoles of Chlamydomonas acidophila (Nishikawa et al, 2003). In V. faba L. the formation of calcium phosphate crystals needs high Ca and P concentrations to allow their precipitation and thus, the question arises on the origin of these elements.…”

Section: Damages On V Faba L As a Response To Metal Contamination: mentioning

confidence: 99%

Response of Vicia faba L. to metal toxicity on mine tailing substrate: Geochemical and morphological changes in leaf and root

Probst

Liu

Fanjul

et al. 2009

Environmental and Experimental Botany

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a b s t r a c tVicia faba L. seeds were grown in a pot experiment on soil, mine tailings, and a mixture of both to mimic field situations in cultivated contaminated areas near mining sites. Metals in the substrates and their translocation in root, stem and leaf tissues were investigated, including morphological responses of plants growing on mine tailings. Metal concentration -and generally bioaccumulation -was in the order: roots > leaves > stems, except Pb and Cd. Translocation was most significant for Zn and Cd, but limited for Pb. Metal concentration in root and leaf was not proportional to that in the substrates, unexpectedly the minimum being observed in the mixed substrate whilst plant growth was retarded by 20% (38% on tailings). Calcium, pH, organic matter and phosphorus were the main influencing factors for metal translocation. The ultrastructure of V. faba L. cells changed a lot in the mine tailings group: root cell walls were thickened with electron dense Pb, Zn and C particles; in chloroplasts, the number of plastoglobuli increased, whereas the thylakoids were swollen and their number decreased in grana. Finally, needleshaped crystalline concretions made of Ca and P, with Zn content, were formed in the apoplast of the plants. The stratagems of V. faba L. undergoing high concentrations of toxic metals in carbonate substrate, suggest root cell wall thickening to decrease uptake of toxic metals, a possible control of metal transport from roots to leaves by synthesizing phytochelators-toxic metal complexes, and finally a control of exceeded Ca and metal concentration in leaves by crystal P formation as ultimate response to stress defense. The geochemical factors influencing metal availability, guaranty a reduction of metal content in plant growing on mixed tailing/soil substrate as far as carbon te are not completely dissolved.

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“…The sequestration of heavy metals using polyP in bacteria is well documented (26,33,35). The hydrolysis of polyP under heavy metal stress has been observed among eukaryotes and prokaryotes alike (32,58). This mechanism may be involved in the detoxification of heavy metals and the excretion of metal-phosphate complexes out of cells (32,33,74).…”

Section: Survival Systems For Environmental Stress: the Relationship mentioning

confidence: 99%

Role of Polyphosphates in Microbial Adaptation to Extreme Environments

Seufferheld

Alvarez

Farı́as

2008

Appl Environ Microbiol

187

107

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Ultrastructural changes in Chlamydomonas acidophila (Chlorophyta) induced by heavy metals and polyphosphate metabolism

Cited by 142 publications

References 17 publications

Responses of the Antarctic microalga Koliella antarctica (Trebouxiophyceae, Chlorophyta) to cadmium contamination

Responses of the Antarctic microalga Koliella antarctica (Trebouxiophyceae, Chlorophyta) to cadmium contamination

Response of Vicia faba L. to metal toxicity on mine tailing substrate: Geochemical and morphological changes in leaf and root

Role of Polyphosphates in Microbial Adaptation to Extreme Environments

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