Ultrastructural changes in ameloblasts from fluoride treated rats

Kruger, B. J.

doi:10.1016/0003-9969(68)90012-5

Cited by 47 publications

(17 citation statements)

References 18 publications

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“…The nucleus was positioned in the basal portion with many mitochondria disposed in the basal cytoplasm. These findings are in agreement with previous reports (Kruger 1968;Walton and Eisenman 1974;Ten cate 1998). On the other hand, large vacuoles distributed throughout the apical cytoplasm resembling cystic formations, and more dilated mitochondria, were observed in the 100 ppm experimental group.…”

Section: Discussionsupporting

confidence: 95%

“…The morphometric results highlighted our morphological findings, as depicted by the increase in mitochondrial volume density. Taken together, there appears to be evidence that NaF at a high dose is able to induce cellular hydropic degeneration as a result of mitochondrial damage (Kruger 1968;Anuradha et al 2001). During enamel development, maturation ameloblasts are responsible for degrading enamel matrix and subsequent enamel mineralization (Ten cate 1998).…”

Section: Discussionmentioning

confidence: 97%

“…In this context, published studies focusing on cellular toxic effects of excess fluoride during enamel development specifically using electron microscopy are few and archaic in the literature. According to some authors, high doses of fluoride may cause dilated mitochondria in ameloblasts of rat incisor (Kruger 1968;Mornstad and Hammarstrom 1978), and the distention and accumulation of secretion material in the rough endoplasmic reticullum (RER) (Kruger 1968). Others have mentioned that excess fluoride may cause accumulation of clear vacuoles (Walton and Eisenmann 1974), reduction of calcium levels in the enamel organ (Eisemann et al 1982), decreased secretion of lysosomal enzymes (Josephen and Fejerskov 1977), fewer bands of modulating ameloblasts, altered rate of ameloblast modulation and slower removal of enamel matrix (Denbesten 1999).…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development

Ribeiro

Hirota²,

Cestari³

et al. 2006

J Mol Hist

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Since a considerable amount of the world population is exposed to high doses of fluoride, it is of special concern to investigate its action mechanisms during dental enamel development. In this study, the toxicity of fluoride in ameloblasts during enamel development was evaluated by means of ultrastructural morphometric analysis. A total of 18 male Wistar rats were distributed into three groups. In Group I, the animals received deionized drinking water ad libitum (negative control) and in Groups II and III, they received sodium fluorided (NaF) drinking water at doses of 7 and 100 ppm ad libitum, respectively, for 6 weeks. Morphometric data were expressed as volume density of the most significant organelles present in the secretory and maturation phases of amelogenesis such as RER, granules, lysosomes, phagic vacuoles, microfilaments and mitochondria. The results showed that the volume density of mitochondria in the 100 ppm experimental group was 29% (P < 0.05) higher than the control group in secretory ameloblasts. No remarkable differences were found in maturation ameloblasts for all organelles evaluated. Taken together, these data indicate that NaF at high doses is able to induce cellular damage in secretory ameloblasts, whereas no noxious effect was observed during maturation stage of amelogenesis as depicted by ultrastructural analysis.

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Section: Discussionsupporting

confidence: 95%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development

Ribeiro

Hirota²,

Cestari³

et al. 2006

J Mol Hist

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“…Disturbances in the mineralization pat tern of the enamel was reported in rats in jected with 7 mg F/kg twice on the 4th and 5th days postnatally [Kruger, 1968] in young adult rats following a few daily injections by 25 mg F/kg [Toda, 1968] and in dogs with 10 mg F/kg beginning 3 days postnatally [Allan, 1962],…”

Section: Morphological Changesmentioning

confidence: 99%

Animal Studies Relating to Caries Inhibition by Fluoride

Larson¹

1977

Caries Res

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“…This effect is held to be of an inhibitory nature, but opinions differ as to which stage or stages in the formation of enamel are partic ularly sensitive. Short-term experiments with rats [Kruger, 1968[Kruger, , 1970bWalton and Eisenmann, 1974;Neiman and Eisenmann, 1975] have demonstrated morphological changes in the secretory ameloblasts and a local interference with the mineralization of the newly formed enamel matrix. Since morphologically similar disturbances have been found also following the short-term administration of several other agents, this type of change is primarily regarded as evi dence that fluoride brings about a largely non-specific cellular response [Neiman and Eisenmann, 1975;Kruger, 1975].…”

mentioning

confidence: 99%

Light and Scanning Electron Microscopy of Fluorosed Enamel from Human Permanent Teeth

Sundström¹,

Myhrberg²

1978

Caries Res

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Acid-etched, ground surfaces of the enamel of 9 fluorosed teeth were studied with scanning electron microscopy, and ground sections from adjacent parts of the same teeth were studied with light microscopy and microradiography. 4 non-fluorosed, intact teeth served as control material. The scanning technique proved to be a sensitive technique for demonstrating slight changes in fluorosed enamel. In comparison with normal enamel, fluorosed enamel showed an overall accentuation of Retzius lines and intraprismatic cross-striations. The outer part of fluorosed enamel often showed a surface zone with a coarsely granular texture in which the prismatic structure was barely detectable, and an underlying subsurface part with a hollowed-out appearance. These findings were discussed in relation to in vivo conditions and the pathogenesis of enamel fluorosis.

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Ultrastructural changes in ameloblasts from fluoride treated rats

Cited by 47 publications

References 18 publications

Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development

Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development

Animal Studies Relating to Caries Inhibition by Fluoride

Light and Scanning Electron Microscopy of Fluorosed Enamel from Human Permanent Teeth

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