Ultrastructural aspects of lipoprotein passage through oocyte envelopes and storage in ooplasm during avian vitellopoiesis

Role of Lipid Metabolism and Signaling in Mammalian Oocyte Maturation, Quality, and Acquisition of Competence

“…Human FF examination revealed the presence of a major amount of HDL (Valckx et al, 2012). These HDL particles are serumderived and are the major lipoproteins present in the FFs because the follicle basement membrane is permeable only to serum proteins ≤300 KDa, thus, excluding LDL and VLDL (Jordanov and Boyadjieva-Michailova, 1974). However, human granulosa-derived lutein cells can also generate LDL and VLDL through ApoB-100 marker.…”

Identification of a Novel Chondroitin-sulfated Collagen in the Membrane Separating Theca and Granulosa Cells in Chicken Ovarian Follicles

“…As this enzyme had no effect on ggBM1, at least under our conditions, the data strongly suggest that ggBM1 is a chondroitin sulfatecontaining proteoglycan. The proteins at 200, 165, 84 (prominent in the samples in lanes [3][4][5][6], and 68 kDa (cf. Fig.…”

“…The coordinated regulation of the synthesis of their components and assembly is one of the prerequisites for the normal growth of the follicles' central giant germ cell, the oocyte. In the final 7-day period of rapid growth of the chicken oocyte, it is surrounded by a concentric arrangement, from the periphery inwards, of (i) vascularized connective tissue representing an extension of the follicle stalk, (ii) the theca proper, which consists of the theca externa, a broad layer of stratified cells with embedded capillaries, and the narrower theca interna, (iii) an acellular layer commonly designated as basal lamina (1,2) or basement membrane (3,4) that functions in follicle compartmentalization, (iv) an epitheloid layer of granulosa cells, and (v) the zona pellucida. Previous studies (5,6) have demonstrated that oocyte growth and development are achieved through the unprecedentedly efficient uptake of hepatically synthesized yolk precursor proteins by multifunctional oocytic plasma membrane receptors belonging to the low density lipoprotein receptor (LR) 3 gene family.…”

“…In the final 7-day period of rapid growth of the chicken oocyte, it is surrounded by a concentric arrangement, from the periphery inwards, of (i) vascularized connective tissue representing an extension of the follicle stalk, (ii) the theca proper, which consists of the theca externa, a broad layer of stratified cells with embedded capillaries, and the narrower theca interna, (iii) an acellular layer commonly designated as basal lamina (1,2) or basement membrane (3,4) that functions in follicle compartmentalization, (iv) an epitheloid layer of granulosa cells, and (v) the zona pellucida. Previous studies (5,6) have demonstrated that oocyte growth and development are achieved through the unprecedentedly efficient uptake of hepatically synthesized yolk precursor proteins by multifunctional oocytic plasma membrane receptors belonging to the low density lipoprotein receptor (LR) 3 gene family. We have shown that at least two such receptors, termed LR8 (7-9) and LRP380 (10), mediate yolk formation via uptake from the serum compartment of macromolecules including very low density lipoprotein (VLDL), vitellogenin, ␣ 2 -macroglobulin, complement component C3, and riboflavin-binding protein (11)(12)(13).…”

High-affinity binding of lower-density lipoproteins to chicken oocyte membranes

“…Krumins and T. F. Roth al., 1972). However, LD lipoprotein has also been implicated in lipid transport into the oocytes (Bacon et al, 1973;Jordanov & Boyadjieva-Michailova, 1974;Perry & Gilbert, 1979). It was therefore necessary to include both VLD lipoprotein and LD lipoprotein in the present study in order to determine by which mechanism lipids enter the oocytes.…”