Ultrastructural aspects of foreign body giant cells generated on different substrates

Harkel, Bas ten; Koopsen, Jelle; Putten, Sander M. van; Veen, H. van der; Picavet, Daisy I.; Vries, Teun J. de; Bank, Ruud A.; Everts, Vincent

doi:10.1016/j.jsb.2016.04.016

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…2C). This is suggestive of a phenomenon seen in mammalian FBR and tuberculosis granulomas, where macrophages converge and fuse, transforming into FBGCs as a consequence of chronic inflammation (Davis et al, 2002; Sheikh et al, 2015; ten Harkel et al, 2016). To examine whether a similar response to foreign body-induced chronic inflammation may be occurring here, we used a Tg( mpeg :mCherry); Tg( mpeg :nlsClover) double transgenic fish to enable visualisation of both cytoplasm and nuclei of macrophages (Fig.…”

Section: Resultsmentioning

confidence: 81%

Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

Gurevich

French

Collin

et al. 2019

Journal of Cell Science

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Implanting biomaterials in tissues leads to inflammation and a foreign body response (FBR), which can result in rejection. Here, we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis. Moreover, we capture macrophages as they fuse to become multinucleate foreign body giant cells (FBGCs) adjacent to the most pro-inflammatory sutures. Genetic and pharmacological dampening of the inflammatory response minimises the FBR (including FBGC generation) and normalises the status of the tissue surrounding these sutures. This model of FBR in adult zebrafish allows us to live image the process and to modulate it in ways that may lead us towards new strategies to ameliorate and circumvent FBR in humans..

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Section: Resultsmentioning

confidence: 81%

Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

Gurevich

French

Collin

et al. 2019

Journal of Cell Science

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“…Release of these deleterious substances was found to lead to the failure of medical implants ( Anderson et al, 2008 ), stressing the need for the development of resistant biomaterials ( Ebert et al, 2005 ). Microscopic analyses revealed that FBGCs firmly adhere to their substrate thereby creating an isolated extracellular environment between the FBGC plasma membrane and the particle surface, called the sealing zone ( ten Harkel et al, 2015 ; ten Harkel et al, 2016 ). The sealing zone can be regarded as an extracellular lysosome in which harmful substances are released in order to degrade the biomaterial ( Vignery, 2005a ).…”

Section: Foreign Body Giant Cell Phenotype Functions and Biological A...mentioning

confidence: 99%

Multinucleated Giant Cells: Current Insights in Phenotype, Biological Activities, and Mechanism of Formation

Ahmadzadeh

Vanoppen

Rosé

et al. 2022

Front. Cell Dev. Biol.

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Monocytes and macrophages are innate immune cells with diverse functions ranging from phagocytosis of microorganisms to forming a bridge with the adaptive immune system. A lesser-known attribute of macrophages is their ability to fuse with each other to form multinucleated giant cells. Based on their morphology and functional characteristics, there are in general three types of multinucleated giant cells including osteoclasts, foreign body giant cells and Langhans giant cells. Osteoclasts are bone resorbing cells and under physiological conditions they participate in bone remodeling. However, under pathological conditions such as rheumatoid arthritis and osteoporosis, osteoclasts are responsible for bone destruction and bone loss. Foreign body giant cells and Langhans giant cells appear only under pathological conditions. While foreign body giant cells are found in immune reactions against foreign material, including implants, Langhans giant cells are associated with granulomas in infectious and non-infectious diseases. The functionality and fusion mechanism of osteoclasts are being elucidated, however, our knowledge on the functions of foreign body giant cells and Langhans giant cells is limited. In this review, we describe and compare the phenotypic aspects, biological and functional activities of the three types of multinucleated giant cells. Furthermore, we provide an overview of the multinucleation process and highlight key molecules in the different phases of macrophage fusion.

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“…Furthermore, macrophages on the samples of TNT/IL‐4/GP/IFN‐γ and TNT/IL‐4/GP exhibited fusion‐induced foreign body giant cells (FBGCs). This material‐dependent inflammatory response is termed the foreign body reaction . Notably, the cytokine IL‐4 has been reported to induce macrophage fusion to FBGCs .…”

Section: Discussionmentioning

confidence: 99%

“…This material‐dependent inflammatory response is termed the foreign body reaction . Notably, the cytokine IL‐4 has been reported to induce macrophage fusion to FBGCs . However, although representing the final stage of macrophage development, few studies are available regarding FBGC function.…”

Section: Discussionmentioning

confidence: 99%

Dual‐inflammatory cytokines on TiO₂ nanotube‐coated surfaces used for regulating macrophage polarization in bone implants

Gao

Yin

et al. 2018

J Biomedical Materials Res

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Excessive immune responses following the use of implantable, biomaterial-based medical devices represent a substantial challenge for treatment efficacy and patient well-being. Specifically, after implantation, pro-inflammatory M1 macrophages are activated by cytokines such as interferon-γ (IFN-γ) followed by anti-inflammatory M2 macrophages polarized by cytokines including interleukin-4 (IL-4), leading to healing and long-term stability of implants. Here, we report the loading of an immunomodulatory cytokine,IL-4, into TiO nanotubes (TNTs) followed by hydrogel coating on the TNTs for subsequent release of IL-4. Finally, IFN-γ was added onto the gel layer to effect rapid release. The release rates of both cytokines from the samples were monitored using an immersion test in phosphate-buffered solution. The cytocompatibility of the sample was evaluated using cultures of osteoblasts and macrophages. Macrophage phenotype switching in vitro was examined via cytokine secretion and gene expression analyses. In vitro testing showed that the sample could stimulate macrophage polarization from the M1 to M2 phenotype at the desired period owing to temporal release of IFN-γ and IL-4. Another biomaterial containing only IL-4 in TNTs was also able to modulate the transformation of M1 to M2 although with weaker effect than that containing IFN-γ and IL-4. The biomaterial may be useful as an osteoimplant in vivo owing to the inflammation caused by a wound or implantation. This study provided biomaterials capable of facilitating smooth M1 to M2 macrophages switching, which might be helpful to research immune responses of tissues to implants and will likely contribute to the development of bone substitute materials. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1878-1886, 2018.

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Ultrastructural aspects of foreign body giant cells generated on different substrates

Cited by 12 publications

References 19 publications

Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

Multinucleated Giant Cells: Current Insights in Phenotype, Biological Activities, and Mechanism of Formation

Dual‐inflammatory cytokines on TiO₂ nanotube‐coated surfaces used for regulating macrophage polarization in bone implants

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Ultrastructural aspects of foreign body giant cells generated on different substrates

Cited by 12 publications

References 19 publications

Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

Live imaging the foreign body response in zebrafish reveals how dampening inflammation reduces fibrosis

Multinucleated Giant Cells: Current Insights in Phenotype, Biological Activities, and Mechanism of Formation

Dual‐inflammatory cytokines on TiO2 nanotube‐coated surfaces used for regulating macrophage polarization in bone implants

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Dual‐inflammatory cytokines on TiO₂ nanotube‐coated surfaces used for regulating macrophage polarization in bone implants