Ultrastructural and X-ray microanalysis of U-937 cells in hypertonia-induced apoptosis

Snigirevskaya, E. S.; Moshkov, Alexey; Yurinskaya, Valentina E.; Aa, Vereninov; Komissarchik, Ya. Yu.

doi:10.1134/s1990519x15020091

Cited by 6 publications

(17 citation statements)

References 46 publications

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“…An increased ratio of intracellular Na+/K+ was detected for majority of the cells entering apoptosis (Figure 1d). These results are consistent with our previous study [5]. …”

supporting

confidence: 83%

“…They demonstrated different immune properties: IGs recognized ABs against the splicing factor SC35 (Figure 1a), meanwhile PRs reacted with antibodies (ABs) against proteins of the proteasome protein complex (Figure 1b). Ultrastructurally PFs observed in this study essentially differed from the ones described earlier [3][4][5][6]. …”

contrasting

confidence: 54%

“…The speckles, were shown previously to be highly dynamic nuclear structures associated with: m-RNA-splicing, transcription, and processing of pre-mRNA [1]. We attribute the described here aggregates to the "HERDS" (heterogeneous ectopic RNP-derived structures) type described by Biggiogera and co-authors [2,3].In contrast to other cytoplasmic aggregates such as aggresomes, processing bodies (Pbs) or stress bodies (SBs) HERDS were observed both in the nucleus, and in cytoplasm [4,5]. They emerge in response to impairment of transcription during apoptosis.…”

mentioning

confidence: 72%

“…In contrast to other cytoplasmic aggregates such as aggresomes, processing bodies (Pbs) or stress bodies (SBs) HERDS were observed both in the nucleus, and in cytoplasm [4,5]. They emerge in response to impairment of transcription during apoptosis.…”

Section: Descriptionmentioning

confidence: 99%

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Ultrastructural Study and X-Ray Microanalysis of Apoptotic Lymphoma Cells U-937

Snigirevskaya¹,

Moshkov²,

Komissarchik³

2017

Biochem Mol biol J

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DescriptionWe studied the ultrastructure and ionic cell contents of human histiocytic lymphoma U-937 cells induced for apoptosis by two external agents, hypertonic shock and etoposide. Despite different mechanisms of action, the deterioration of cellular functions triggered by these agents followed similar patterns. The most dramatic changes-condensation of the chromatin and formation of "crescent-shaped nuclei", nucleus fragmentation, disappearance of nucleoli, and appearance of aggregated particles in the interchromatin nuclear space were observed in the nucleus. The aggregated particles observed in the interchromatin nuclear space, were lacking limiting membrane and presumably derived from the aggregates of interchromatin granules (IGs), so called speckles. The speckles, were shown previously to be highly dynamic nuclear structures associated with: m-RNA-splicing, transcription, and processing of pre-mRNA [1]. We attribute the described here aggregates to the "HERDS" (heterogeneous ectopic RNP-derived structures) type described by Biggiogera and co-authors [2,3].In contrast to other cytoplasmic aggregates such as aggresomes, processing bodies (Pbs) or stress bodies (SBs) HERDS were observed both in the nucleus, and in cytoplasm [4,5]. They emerge in response to impairment of transcription during apoptosis. In case of cellular physiological modifications, e.g. under spermiogenesis or animal hibernation, this process can be reversible [2,3]. Ultrastructural analysis and immune electron microscopy of the aggregates observed in U-937 cell line allowed identification of three types of nuclear particles dominating in these aggregates: interchromatin granules (IGs) of 10 x 40 nm, proteasomes (PRs) of 10-15 × 30 nm, and long perichromatin fibers (PFs) [4]. Both IGs and PRs were rod-like shaped particles of variable size. They demonstrated different immune properties: IGs recognized ABs against the splicing factor SC35 (Figure 1a), meanwhile PRs reacted with antibodies (ABs) against proteins of the proteasome protein complex (Figure 1b). Ultrastructurally PFs observed in this study essentially differed from the ones described earlier [3][4][5][6]. Figure 1Apoptotic cells (ultrastructure and X-ray microanalysis). a-intranuclear aggregate, HERDS, contains interchromatin granules, proteasomes, and perichromatin fibers. Arrows indicate the gold labels of SC35. N-nucleus, C-cytoplasm, NEnuclear envelope, IS-interchromatin space; b-loose aggregate of proteasomes in cytoplasm labeled with pABs against proteasome and immune gold. NP-nuclear pore; c-perichromatin fibers (PFs) (encircled by ovals) in nucleus and cytoplasm; d-histogramm of Na+ and K+ elemental contents in U-937 cells (control n=5, apoptosis n=5). X-ray spectra of elements were collected at accelerating voltage of 80 kv for 300 s. Data shown as means ± SEM.PFs on sections contrasted with uranyl-acetate were seen as elongated electron dense particles 12-13 nm thick with a periodic structure with a period of 10 nm (Figure 1c) [7]. PFs were formed by 4 nm thick hel...

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“…An increased ratio of intracellular Na+/K+ was detected for majority of the cells entering apoptosis (Figure 1d). These results are consistent with our previous study [5]. …”

supporting

confidence: 83%

contrasting

confidence: 54%

mentioning

confidence: 72%

Section: Descriptionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrastructural Study and X-Ray Microanalysis of Apoptotic Lymphoma Cells U-937

Snigirevskaya¹,

Moshkov²,

Komissarchik³

2017

Biochem Mol biol J

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“…A and B). Later on, the numerous blebs and apoptotic bodies (ApBs) are formed and bud off PM (Bonnano et al, ; Snigirevskaya et al, ). The ApBs retain intact PM and contain remnants of the cell nucleus and organelles (Fig.…”

Section: Apoptosis and Plasma Membrane (Pm)mentioning

confidence: 99%

Ultrastructural traits of apoptosis

Snigirevskaya

Komissarchik

2019

Cell Biology International

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This review summarizes original and literature data on changes in the ultrastructure of major cell organelles during apoptosis obtained by transmission electron microscopy. Organelles that make the most crucial contribution to the initiation of apoptosis: plasma membrane, mitochondria, proteasomes, Golgi apparatus, and endoplasmic reticulum, were of our prime attention. The nucleus and cytoskeleton that undergo essential changes, were considered as well. Special attention was paid to the data on ultrastructural changes in the cell organelles observed recently by electron microscopic tomography and correlative microscopy, in particular, to remodeling of mitochondrial crista junctions and microtubules during the execution phase of apoptosis.

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Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers

Snigirevskaya

Komissarchik

2017

Acta Histochemica

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Ultrastructural and X-ray microanalysis of U-937 cells in hypertonia-induced apoptosis

Cited by 6 publications

References 46 publications

Ultrastructural Study and X-Ray Microanalysis of Apoptotic Lymphoma Cells U-937

Ultrastructural Study and X-Ray Microanalysis of Apoptotic Lymphoma Cells U-937

Ultrastructural traits of apoptosis

Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers

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