Ultrastructural and ultracytochemical changes in the lungs in diabetic rats

Lysenko, Lidiа; Lysenko, A. I.; Kulik, V. P.

doi:10.1007/bf00839755

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…8 Furthermore, in animal models with STZ-induced diabetes there is thickening of the alveolar epithelial and pulmonary capillary basal lamina, leading to measurable loss of respiratory efficiency [4][5][6] as well as structural insufficiency of type-II pneumocytes, polymorphonuclear leucocytes and alveolar macrophages. 7 Decreased capacity of the antioxidative defence system and increased oxidative stress were seen in the lung of diabetic rats and rabbits. 10,11 Nitric oxide, produced by nitric oxide synthase (NOS) serves as a ubiquitous mediator in many physiological lung functions, including regulation of vascular and bronchial tone, immunocompetence and neuronal signalling.…”

Section: Introductionmentioning

confidence: 98%

The relationship between nitric oxide and leptin in the lung of rat with streptozotocin‐induced diabetes

Öztay

Kandil

Gúrel

et al. 2007

Cell Biochemistry & Function

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Lung structural changes and immunoreactivity of endothelial (eNOS)- and inducible nitric oxide synthase (iNOS) were investigated by light microscopy in lungs of treated and untreated diabetic rats. Diabetes was induced by a single intraperitoneal (i.p.) injection of 65 mg kg(-1) streptozotocin (STZ) in Wistar albino male rats. Diabetic rats received daily i.p. doses of dexamethasone (2 mg kg(-1)), leptin (0.5 microg kg(-1)) and intramuscular insulin (20 U kg(-1)) or a combination of these drugs for 1 week starting 4 weeks after the STZ injections. After treatment, the blood levels of glucose, leptin, insulin and nitrate/nitrite (NO(3) (-)/NO(2) (-)) were measured. Dilatation of alveoli and alveolar ducts, partial alveolar wall thickening and increased eNOS- and iNOS characterized the diabetic rat lungs. High blood glucose and nitrate/nitrite levels as well as low insulin and leptin levels were also present. Treatment with insulin, dexamethasone and a combination of these drugs resulted in improvement of the structural and immunohistochemical abnormalities. The most effective treatment was insulin therapy. Leptin administration resulted in increased relative amounts of extracellular material, which led to noticeable respiratory efficiency in the diabetic rat lungs. All treatments except leptin lowered blood glucose levels. The combination of insulin and dexamethasone increased blood leptin and insulin, while the remaining diabetic rats had blood with low leptin and insulin concentrations. These results suggest that therapy with insulin plus dexamethasone but not therapy with leptin is beneficial for diabetics.

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Section: Introductionmentioning

confidence: 98%

The relationship between nitric oxide and leptin in the lung of rat with streptozotocin‐induced diabetes

Öztay

Kandil

Gúrel

et al. 2007

Cell Biochemistry & Function

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Role of microvillar cell surfaces in the regulation of glucose uptake and organization of energy metabolism

Lange¹

2002

American Journal of Physiology-Cell Physiology

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Experimental evidence suggesting a type of glucose uptake regulation prevailing in resting and differentiated cells was surveyed. This type of regulation is characterized by transport-limited glucose metabolism and depends on segregation of glucose transporters on microvilli of differentiated or resting cells. Earlier studies on glucose transport regulation and a recently presented general concept of influx regulation for ions and metabolic substrates via microvillar structures provide the basic framework for this theory. According to this concept, glucose uptake via transporters on microvilli is regulated by changes in the structural organization of the microfilament bundle, which is acting as a diffusion barrier between the microvillar tip compartment and the cytoplasm. Both microvilli formation and the switch of glucose metabolism from "metabolic regulation" to "transport limitation" occur during differentiation. The formation of microvillar cell surfaces creates the essential preconditions to establish the characteristic functions of specialized tissue cells including the coordination between glycolysis and oxidative phosphorylation, regulation of cellular functions by external signals, and Ca(2+) signaling. The proposed concept integrates various aspects of glucose uptake regulation into a ubiquitous cellular mechanism involved in regulation of transmembrane ion and substrate fluxes.

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Ultrastructural and ultracytochemical changes in the lungs in diabetic rats

Cited by 2 publications

References 11 publications

The relationship between nitric oxide and leptin in the lung of rat with streptozotocin‐induced diabetes

The relationship between nitric oxide and leptin in the lung of rat with streptozotocin‐induced diabetes

Role of microvillar cell surfaces in the regulation of glucose uptake and organization of energy metabolism

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