Ultrastructural and SINS analysis of the cell wall integrity response of<i>Aspergillus nidulans</i>to the absence of galactofuranose

Bakir, Görkem; Girouard, Benoit E.; Johns, Robert W.; Findlay, Catherine Rui Jin; Bechtel, Hans A.; Eisele, Max; Kaminskyj, Susan G. W.; Dahms, Tanya E. S.; Gough, Kathleen

doi:10.1039/c8an01591k

Cited by 8 publications

(11 citation statements)

References 45 publications

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“…The cell walls of fungi were also studied. Bakir et al 77 identified the nanoscale structure and chemical components of the cell wall of the gene deletion strains by s-SNOM. Li et al 61 used PFIR with a resolution of 6 nm to reveal the chemical composition and distribution of yeast cell wall particles.…”

Section: Test Results and Analysismentioning

confidence: 99%

Review on near-field detection technology in the biomedical field

et al. 2023

Adv. Photon. Nexus

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We review the recent biomedical detection developments of scanning near-field optical microscopy (SNOM), focusing on scattering-type SNOM, atomic force microscope-based infrared spectroscopy, peak force infrared microscopy, and photo-induced force microscopy, which have the advantages of label-free, noninvasive, and specific spectral recognition. Considering the high water content of biological samples and the strong absorption of water by infrared waves, we divide the relevant research on these techniques into two categories: one based on a nonliquid environment and the other based on a liquid environment. In the nonliquid environment, the chemical composition and structural information of biomedical samples can be obtained with nanometer resolution. In the liquid environment, these techniques can be used to monitor the dynamic chemical reaction process and track the process of chemical composition and structural change of single molecules, which is conducive to exploring the development mechanism of physiological processes. We elaborate their experimental challenges, technical means, and actual cases for three microbiomedical samples (including biomacromolecules, cells, and tissues). We also discuss the prospects and challenges for their development. Our work lays a foundation for the rational design and efficient use of near-field optical microscopy to explore the characteristics of microscopic biology.

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Section: Test Results and Analysismentioning

confidence: 99%

Review on near-field detection technology in the biomedical field

et al. 2023

Adv. Photon. Nexus

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“…Joined by other modalities, s-SNOM has been participating in fungi and bacteria research as a superresolution chemical resolving tool to reveal nanoscale chemical heterogeneities. Bakir et al have used s-SNOM with synchrotron radiation to obtain mid-IR spectra from specific points on single fungi cells of Aspergillus nidulans [83]. In addition to architecture alterations of two gene deletion strains compared to the wild type A. nidulans, compositional changes were also discovered by a series of near-field spectra.…”

Section: S-snom In the Characterization Of Virus Fungi And Bacteriamentioning

confidence: 99%

Super-resolution mid-infrared spectro-microscopy of biological applications through tapping mode and peak force tapping mode atomic force microscope

Wang

Xie

2022

Advanced Drug Delivery Reviews

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Tracking Cell Wall Changes With Correlative Afm-microspectroscopymentioning

confidence: 99%

“…A relatively new correlative method uses synchrotron-based infrared nanospectroscopy (SINS) to illuminate an AFM tip for the dual purpose of nanoscale imaging while using near-field infrared spectroscopy to report on local chemical groups just nanometers below the tip (Bakir et al, 2019). SINS of the opportunistic pathogen Aspergillus nidulans and its mutants lacking the sugar galactofuranose, involved in pathogenicity and cell wall function, showed changes in cell wall biochemistry and surface roughness, best attributed to the cell wall integrity pathway (Bakir et al, 2019). Kochan et al (2018) used a similar approach to monitor dynamic changes in the cell wall of six bacterial species, including pathogenic S. aureus and E. coli, during cell division, showing changes to complex carbohydrate and phosphodiester groups, including peptidoglycans and teichoic acid, and enabling discrimination of Gram-positive and Gram-negative cell wall components.…”

Section: Tracking Cell Wall Changes With Correlative Afm-microspectroscopymentioning

confidence: 99%

AFM-Based Correlative Microscopy Illuminates Human Pathogens

Bhat

Price

Dahms

2021

Front. Cell. Infect. Microbiol.

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Microbes have an arsenal of virulence factors that contribute to their pathogenicity. A number of challenges remain to fully understand disease transmission, fitness landscape, antimicrobial resistance and host heterogeneity. A variety of tools have been used to address diverse aspects of pathogenicity, from molecular host-pathogen interactions to the mechanisms of disease acquisition and transmission. Current gaps in our knowledge include a more direct understanding of host-pathogen interactions, including signaling at interfaces, and direct phenotypic confirmation of pathogenicity. Correlative microscopy has been gaining traction to address the many challenges currently faced in biomedicine, in particular the combination of optical and atomic force microscopy (AFM). AFM, generates high-resolution surface topographical images, and quantifies mechanical properties at the pN scale under physiologically relevant conditions. When combined with optical microscopy, AFM probes pathogen surfaces and their physical and molecular interaction with host cells, while the various modes of optical microscopy view internal cellular responses of the pathogen and host. Here we review the most recent advances in our understanding of pathogens, recent applications of AFM to the field, how correlative AFM-optical microspectroscopy and microscopy have been used to illuminate pathogenicity and how these methods can reach their full potential for studying host-pathogen interactions.

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Ultrastructural and SINS analysis of the cell wall integrity response ofAspergillus nidulansto the absence of galactofuranose

Abstract: With lethal opportunistic fungal infections on the rise, it is imperative to explore new methods to examine virulence mechanisms.

Cited by 8 publications

References 45 publications

Review on near-field detection technology in the biomedical field

Review on near-field detection technology in the biomedical field

Super-resolution mid-infrared spectro-microscopy of biological applications through tapping mode and peak force tapping mode atomic force microscope

AFM-Based Correlative Microscopy Illuminates Human Pathogens

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