Ultrastructural and molecular characterization of Pneumocystis carinii isolated from a rhesus monkey (Macaca mulatta)

Durand-Joly, I.; Wakefield, Ann E.; Palmer, R. J.; Denis, Cecile-Marie; Creusy, C.; Fleurisse, L.; Ricard, I.; Gut, J. P.; Dei‐Cas, Eduardo

doi:10.1080/714030897

Cited by 15 publications

(17 citation statements)

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“…Numerous gene fragments were compared in Pneumocystis from humans and other primates, rodents, rabbits and insectivores. Consistently it was found that specific sequences could be attributed to pathogens from different host species (Banerji et al , 1995; Mazars et al , 1995; Laakkonen, 1998; Wakefield et al , 1998; Denis et al , 2000; Durand‐Joly et al , 2000; Guillot et al , 2001). Karyotypic divergence was found among Pneumocystis strains from diverse hosts (Keely et al , 2004).…”

Section: Introductionmentioning

confidence: 53%

“…mustelae , P. murina (Hunter & Wakefield, 1996), P. carinii f.sp. macaca from Indian Macaca rhesus (Durand‐Joly et al , 2000), accession nos AF395579, AF395580, AF395582, AF395584, AF395578, AF395583, AF395574, AF395585, AF395576, AF395575, AF395577, AF395581; thymidylate synthase, rabbit‐derived Pneumocystis , P. jirovecii , P. murina (Mazars et al , 1995), accession nos ; 5‐enolpyruvylshikimate‐3‐phosphate synthase (AROM), accession nos U31054, U31055, L18918, U31056, U31053; internal transcribed spacer 1 (ITS1), accession nos DQ010098, AF013806, L27658, AY532651, AF288827; internal transcribed spacer 2 (ITS2), accession nos DQ010098, AF013821, L27658, AY53651, AF288835; Heat shock protein 70 (HSP70), accession nos DQ435616, U80970, U80968, U80969, AY382182; beta‐tubulin (β‐tub), rabbit‐derived Pneumocystis (this paper), accession nos AF170964, X62113.…”

Section: Pneumocystis Taxonomy At the Species Level: Sequence Comparimentioning

confidence: 99%

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Pneumocystis oryctolagisp. nov., an uncultured fungus causing pneumonia in rabbits at weaning: review of current knowledge, and description of a new taxon on genotypic, phylogenetic and phenotypic bases

et al. 2006

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The genus Pneumocystis comprises noncultivable, highly diversified fungal pathogens dwelling in the lungs of mammals. The genus includes numerous host-species-specific species that are able to induce severe pneumonitis, especially in severely immunocompromised hosts. Pneumocystis organisms attach specifically to type-1 epithelial alveolar cells, showing a high level of subtle and efficient adaptation to the alveolar microenvironment. Pneumocystis species show little difference at the light microscopy level but DNA sequences of Pneumocystis from humans, other primates, rodents, rabbits, insectivores and other mammals present a host-species-related marked divergence. Consistently, selective infectivity could be proven by cross-infection experiments. Furthermore, phylogeny among primate Pneumocystis species was correlated with the phylogeny of their hosts. This observation suggested that cophylogeny could explain both the current distribution of pathogens in their hosts and the speciation. Thus, molecular, ultrastructural and biological differences among organisms from different mammals strengthen the view of multiple species existing within the genus Pneumocystis. The following species were subsequently described: Pneumocystis jirovecii in humans, Pneumocystis carinii and Pneumocystis wakefieldiae in rats, and Pneumocystis murina in mice. The present work focuses on Pneumocystis oryctolagi sp. nov. from Old-World rabbits. This new species has been described on the basis of both biological and phylogenetic species concepts.

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Section: Introductionmentioning

confidence: 53%

Section: Pneumocystis Taxonomy At the Species Level: Sequence Comparimentioning

confidence: 99%

Pneumocystis oryctolagisp. nov., an uncultured fungus causing pneumonia in rabbits at weaning: review of current knowledge, and description of a new taxon on genotypic, phylogenetic and phenotypic bases

et al. 2006

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“…Several recent studies indicated that Pneumocystis organisms derived from mouse, rat, monkey, and human hosts form genetically distinct groups [2–4,9,10,12,22]. Most of these studies used a single gene to infer these groups, but since a single gene may not contain enough information to resolve these relationships, these studies should be considered with caution.…”

Section: Resultsmentioning

confidence: 99%

Evolution and Speciation of Pneumocystis

Keely

Fischer

Stringer

2003

J Eukaryotic Microbiology

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“…The large subunit of the mitochondrial ribosomal RNA was amplified from an existing cDNA clone (kindly provided by Dr. K. Norris, University of Pittsburgh), using previously described primers [15]. The amplified product (364 bp) was subcloned into the pGEMT vector and DNA sequenced.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Increased expression of interferon-inducible genes in macaque lung tissues during simian immunodeficiency virus infection

Schaefer

Fuller

Basu

et al. 2006

Microbes and Infection

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Pulmonary infections and dysfunction are frequent outcomes during the development of immunodeficiency associated with human immunodeficiency virus type 1 (HIV-1) infection, and obtaining a better understanding of the immunologic changes that occur in lungs following HIV-1 infection will provide a foundation for the development of further intervention strategies. We sought here to identify changes in the pulmonary immune environment that arise during simian immunodeficiency virus (SIV) infection of rhesus macaques, which serves as an excellent model system for HIV-1 infection and disease. To examine the gene expression profiles of macaque lung tissues following infection with the pathogenic SIV/DeltaB670 isolate, we performed cDNA microarray hybridizations with lung total RNAs using two commercially available cDNA arrays and a custom-fabricated, immunologically focused macaque cDNA microarray. In situ hybridization and real-time RT-PCR were performed to provide additional analyses of gene expression. Among the genes exhibiting the highest level of induction in lung tissues were the IFN-gamma-inducible chemokines, CXCL10/IP-10 and CXCL9/Mig. In situ hybridization and real-time RT-PCR strongly supported these findings. Correlation analyses revealed that the levels of expression of IFN-gamma, CXCL9/Mig, and CXCL10/IP-10 mRNAs were all strongly positively correlated, and that CXCL10/IP-10 mRNA and Pneumocystis carinii rRNA were positively correlated. Taken together, these findings demonstrate that inflammatory chemokines are among the most differentially expressed mRNAs in macaque lung tissues during systemic SIV infection of rhesus macaques, and provide insight into the complicated events occurring in the lung tissues during HIV-1 infection in humans.

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Ultrastructural and molecular characterization of Pneumocystis carinii isolated from a rhesus monkey (Macaca mulatta)

Cited by 15 publications

References 0 publications

Pneumocystis oryctolagisp. nov., an uncultured fungus causing pneumonia in rabbits at weaning: review of current knowledge, and description of a new taxon on genotypic, phylogenetic and phenotypic bases

Pneumocystis oryctolagisp. nov., an uncultured fungus causing pneumonia in rabbits at weaning: review of current knowledge, and description of a new taxon on genotypic, phylogenetic and phenotypic bases

Evolution and Speciation of Pneumocystis

Increased expression of interferon-inducible genes in macaque lung tissues during simian immunodeficiency virus infection

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