Ultrastructural and mechanical properties of electrically inexcitable skeletal muscle fibers of the crustacean Atya lanipes

Bonilla, Mauricio R.; García, Maria C.; Orkand, Paula M.; Zuazaga, C.

doi:10.1016/0040-8166(92)90068-i

Cited by 7 publications

(2 citation statements)

References 31 publications

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“…Vertebrate muscle cell classification of striated and smooth muscle is not quite adequate for invertebrate muscles. Transversely striated muscle cells are present in arthropods (Smith, 1961;Bonilla et al, 1992). Obliquely striated muscle is found in nematodes (Rosenbluth, 1965), annelids (Hanson, 1957;de Eguileor et al, 1987;Royuela et al, 19951, and molluscs (Morrison and Odense, 1974;Matsuno and Kuga, 1989).…”

Section: Sophila Melumguster Muscle Oligochaete Annelid Musclementioning

confidence: 99%

Immunocytochemical electron microscopic study and western blot analysis of troponin in striated muscle of the fruit flyDrosophila melanogaster and in several muscle cell types of the earthwormEisenia foetida

et al. 1996

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Background There is little information about troponin in invertebrate muscles, and no previous references to this protein in annelid muscles have been found. The aim of this paper was to study the presence and distribution of troponin in different muscle cell types from the earthworm Eisenia foetida (the muscular body wall, and the inner and outer muscular layer of the pseudoheart). These results were compared with those obtained in the transversely striated muscle of Drosophila melanogaster and in skeletal and smooth muscles of the mouse. Methods Immunocytochemical electron microscopic study and Western blot analysis using anti‐TnT antibodies were employed in this study. Results Troponin immunoreaction was detected in the mouse skeletal muscle, the fly flight muscle, and earthworm obliquely striated muscles (body wall musculature and inner muscular layer of the pseudoheart). Immunolabeling for TnT in all these muscle cells appeared in moderate amounts at any point along the sarcomere length, except for the central zone of the A band (H band). This suggests that troponin molecules were located along the thin filaments. The density of immunogold particles was similar in the three muscles, and thus the amount of troponin in each muscle type was proportional to the number and length of actin filaments in each. Troponin was found in neither the mouse smooth muscle nor the outer muscular layer of the earthworm pseudoheart. The latter muscle showed an ultrastructural pattern that was intermediate between obliquely striated and smooth muscle. The estimated molecular weight for TnT in the earthworm was 55 kDa; this is higher than the weight of this protein in the mouse skeletal muscle (40 kDa) but similar to that of the D. melanogaster muscle (52 kDa). Conclusions Troponin is present in both types of striated muscle (transversely striated and obliquely striated) of the earthworm with a distribution that is very similar to that observed in the mammalian striated muscle. As in vertebrates, troponin is absent in the smooth muscle of the earthworm. Discrepancies in the classification of some invertebrate muscles are common in the literature, and the use of distinctive markers, such as troponin, may improve our understanding of the nature and properties of many invertebrate muscles showing an ultrastructural pattern that does not resemble any of the classic muscle types. © 1996 Wiley‐Liss, Inc.

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Section: Sophila Melumguster Muscle Oligochaete Annelid Musclementioning

confidence: 99%

Immunocytochemical electron microscopic study and western blot analysis of troponin in striated muscle of the fruit flyDrosophila melanogaster and in several muscle cell types of the earthwormEisenia foetida

et al. 1996

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“…In crustacean skeletal muscle CICR is proposed to be the mechanism of excitation-contraction coupling (Györke and Palade, 1992). Unlike in other arthropod skeletal muscles, the superficial abdominal flexor muscle of the crustacean Atya lanipes is electrically unexcitable (Monterrubio et al, 2000;Bonilla et al, 1992 (Brown and Starrat, 1975). In insects and crustaceans proctolin stimulates skeletal and visceral muscle and potentiates nerve-stimulated contractions of skeletal muscles (Facciponte et al, 1996;Bartos et al, 1994;Lange, 2002).…”

Section: Ties Whilementioning

confidence: 99%