Ultrastructural and immunohistochemical study of endocrine cells in the midgut of the cockroach Blaberus craniifer (Insecta, Dictyoptera)

Andries, J.C.; Tramu, G.

doi:10.1007/bf00222342

Cited by 49 publications

(17 citation statements)

References 35 publications

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“…However, Leu-callatostatin immunoreactivity was found in endocrine cells in a narrow part of the midgut immediately anterior to the point ofevagination ofthe Malpighian tubules (28 (30), only immunocytochemical and morphological similarities to the gut endocrine system in vertebrates lead us to call these midgut cells "endocrine." The ultrastructure ofmidgut endocrine cells has been described in other cockroaches, Nauphoeta cinerea (31), P. americana (32), and Blaberus craniifer (33), in which at least 10 cell types were distinguishable by the shape and size of their secretory granules. Ultrastructural images of exocytosis of secretory granules from endocrine cells of P. americana were presented by Endo and Nishiitsutsuji-Uwo (34 …”

Section: Discussionmentioning

confidence: 99%

Expression of the allatostatin gene in endocrine cells of the cockroach midgut.

Reichwald

Unnithan

Davis

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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Cockroach allatostatins are neuropeptides that have been isolated from the brain of Diploptera punctata and shown to inhibit juvenile hormone production by the corpora allata. Enzyme-linked immunoassay and Immunocytochemistry with antisera to two allatostatins, ASB2 (AYSYV-SEYKRLPVYNFGL-NH2) and ASAL (APSGAQRLYGFGL-NH2), revealed that allatostatins were located not only in the insect brain but also in several peripheral tissues Including the cockroach midgut and hindgut. Allatostatin-like immunoreactivity was found in nerve fibers of the stomatogastric nervous system as well as in intrinsic endocrine cells of the midgut.Midgut extracts were shown to be biologically active in an allatostatin bioassay and to contain several alatostatin-like peptides, including the octadecapeptide ASB2, which was identified by mass spectrometry following HPLC purification.Reverse transcription of brain mRNA followed by PCR with degenerate oligonucleotides for ASB2 and ASAL yielded a 338-bp fragment of the allatostatin gene that encoded six allatostatins. In situ hybridization with this probe confirmed that an allatostatin gene is expressed in intrinsic endocrine cells of the midgut. Reverse transcription of midgut mRNA followed by PCR and sequencing of the product revealed that the same gene is expressed in the midgut and in the brain. Allatostatins are thus an example of insect "brain-gut peptides" and we suggest that their function may not be restricted to the regulation of juvenile hormone production.

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Section: Discussionmentioning

confidence: 99%

Expression of the allatostatin gene in endocrine cells of the cockroach midgut.

Reichwald

Unnithan

Davis

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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“…Lepidopteran midgut is composed of four types of cells: columnar, goblet, endocrine and regenerative (ANDRIÉS and TRAMU, 1985;LEHANE and BILLINGSLEY, 1996;PINHEIRO et al, 2003;LEVY et al, 2004). Endocrine cells have been observed between epithelial cells in the midgut epithelium of the majority of insects, but they have been described mainly for Lepidoptera, Ephemeroptera, Trichoptera and Plecoptera (MONTUENGA et al, 1989;NISHIITSUTSUJI-UWO and ENDO, 1981;ROST-ROSZKOWSKA, 2008).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of two Types of Endocrine Cells in the Midgut Epithelium of Spodoptera Exiqua Hűbner, 1808 (Insecta, Lepidoptera, Noctuidae)

Rost‐Roszkowska¹,

Chechelska²,

Frądczak³

et al. 2008

Zoologica Poloniae

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“…However, some differences in relation to the number, type, and distribution of these cells are observed among the studied species, possibly indicating important differences in the digestive physiology of these insects (Andriès & Tramu, 1985).…”

Section: Introductionmentioning

confidence: 95%

“…However, ultrastructural (Priester, 1971;Andriès, 1976;Reinhardt, 1976;Cassier & Fain-Maurel, 1977;Endo et al, 1982;Brown et al, 1985;Andriès & Tramu, 1985;Montuenga et al, 1989;Stoffolano et al, 1989) and immunocytochemical studies have demonstrated the presence of these cells in the midgut epithelium of various insect species (Andriès, 1976;Endo et al, 1982;Andriès & Tramu, 1984Iwanaga et al, 1986;Brown et al, 1986;Glattli et al, 1987;Andriès & Beauvillain, 1988;Montuenga et al, 1989;Zitnan et al, 1993;An et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of the midgut endocrine cells in Melipona quadrifasciata anthidioides (Hymenoptera, Apidae)

2003

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In this study we describe the ultrastructure of the endocrine cells observed in the midgut of M. quadrifasciata anthidioides. This bee has two types of endocrine cells, which are numerous on the posterior midgut region. Cells of the closed type are smaller and have irregular secretory granules with lower electrondensity than those of the open cell type. The open cell type has elongated mitochondria mainly on the basal area, where most of the secretory granules are also found. Besides the secretion granules and mitochondria, endocrine cells in this species have well-developed autophagic vacuoles and Golgi complex elements.Key words: insect midgut, endocrine cells, ultrastructure, secretory granules, stingless bee. RESUMO Ultra-estrutura das células enteroendócrinas do intestino médio deMelipona quadrifasciata anthidioides (Hymenoptera, Apidae)Neste estudo descrevemos a ultra-estrutura das células enteroendócrinas observadas no intestino médio de M. quadrifasciata anthidioides, abelha na qual ocorrem dois tipos de células enteroendócrinas, concentradas na região posterior do intestino médio. As células do tipo fechado são menores e possuem grânulos de secreção menos eletrodensos e menos regulares do que as células do tipo aberto. As células do tipo aberto possuem ainda longas mitocôndrias concentradas principalmente na região basal, onde também se concentra a maior parte dos grânulos de secreção. Além dos grânulos de secreção e das mitocôndrias, as células endócrinas nessa espécie possuem grandes vacúolos autofágicos e elementos do complexo de Golgi bem desenvolvidos.Palavras-chave: intestino médio, células enteroendócrinas, ultra-estrutura, grânulos de secreção, abelhas sem ferrão.

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Ultrastructural and immunohistochemical study of endocrine cells in the midgut of the cockroach Blaberus craniifer (Insecta, Dictyoptera)

Cited by 49 publications

References 35 publications

Expression of the allatostatin gene in endocrine cells of the cockroach midgut.

Expression of the allatostatin gene in endocrine cells of the cockroach midgut.

Ultrastructure of two Types of Endocrine Cells in the Midgut Epithelium of Spodoptera Exiqua Hűbner, 1808 (Insecta, Lepidoptera, Noctuidae)

Ultrastructure of the midgut endocrine cells in Melipona quadrifasciata anthidioides (Hymenoptera, Apidae)

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