Ultrastructural and cytochemical characterization of human cord blood cells

Mikami, Takafumi; Eguchi, Mitsuoki; Kurosawa, Hidemitsu; Sato, Yuya; Sugita, Kenichi; Suzumura, Hiroshi; Tadokoro, N; Watanabe, Hiroshi; Inaba, Noriyuki

doi:10.1007/s007950200012

Cited by 17 publications

(22 citation statements)

References 14 publications

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“…This finding suggests the potential of a nonhematopoietic subpopulation within the mononuclear hUCB cells to become neural cells. hUCB cells have the more immature morphology of myelomonocytic cells with small numbers of mature neutrophils, as compared to the adult originated blood cells and BM cells [36]. A higher proportion of immature T-cells and a lesser number of mature memory [37,38] and CD56 + cytotoxic [38] T-cells in hUCB, as compared to that of adult sources, also reflects the immunological immaturity of hUCB.…”

Section: Cell Composition and Mononuclear Fractionsmentioning

confidence: 97%

Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury

Park

Lee

Borlongan

et al. 2010

Stem Cell Rev and Rep

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Spinal cord injury (SCI) develops primary and secondary damage to neural tissue and this often results in permanent disability of the motor and sensory functions. However, there is currently no effective treatment except methylprednisolone, and the use of methylprednisolone has also been questioned due to its moderate efficacy and the drug's downside. Regenerative medicine has remarkably developed since the discovery of stem cells, and many studies have suggested the potential of cell-based therapies for neural injury. Especially, the therapeutic potential of human umbilical cord blood cells (hUCB cells) for intractable neurological disorders has been demonstrated using in vitro and vivo models. The hUCB cells are immune naïve and they are able to differentiate into other phenotypes, including the neural lineage. Their ability to produce several neurotropic factors and to modulate immune and inflammatory reactions has also been noted. Recent evidence has emerged suggesting alternative pathways of graft-mediated neural repair that involve neurotrophic effects. These effects are caused by the release of various growth factors that promote cell survival, angiogenesis and anti-inflammation, and this is all aside from a cell replacement mechanism. In this review, we present the recent findings on the stemness properties and the therapeutic potential of hUCB as a safe, feasible and effective cellular source for transplantation in SCI. These multifaceted protective and restorative effects from hUCB grafts may be interdependent and they act in harmony to promote therapeutic benefits for SCI. Nevertheless, clinical studies with hUCB are still rare because of the concerns about safety and efficiency. Among these concerns, the major histocompatibility in allogeneic transplantation is an important issue to be addressed in future clinical trials for treating SCI.

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Section: Cell Composition and Mononuclear Fractionsmentioning

confidence: 97%

Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury

Park

Lee

Borlongan

et al. 2010

Stem Cell Rev and Rep

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“…These procedures and descriptions of the cell states have been published (Olins et al 1998;Olins and Olins 2004). Scale bars 500 nm Mikami et al 2002;Olins et al 1998;Smith and O'Hara 1967;Smith and O'Hara 1968 Fetal thymocytes Sebuwufu 1966 Pathological cells…”

Section: Occurrencementioning

confidence: 99%

Nuclear envelope-limited chromatin sheets (ELCS) and heterochromatin higher order structure

Olins

2009

Chromosoma

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The interphase nucleus and nuclear envelope can acquire a myriad of shapes in normal or pathological cell states. There exist a wide variety of indentations and invaginations, of protrusions and evaginations. It has been difficult to classify and name all of these nuclear shapes and, consequently, a barrier to understanding the biochemical and biophysical causes. This review focuses upon one type of nuclear envelope shape change, named "nuclear envelope-limited chromatin sheets" (ELCS), which appears to involve exaggerated nuclear envelope growth, carrying with it one or more layers of approximately 30 nm diameter heterochromatin. A hypothesis on the formation of ELCS is proposed, relating higher order heterochromatin structure in an interphase nucleus, nuclear envelope growth, and nuclear envelope-heterochromatin interactions.

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“…Hematopoietic progenitors from hUCB are rich in the most primitive stem cells and are able to produce long-term repopulation of blood lineages (17)(18)(19). Compared to bone marrow cells, hUCB cells have a more immature morphology of the myelomonocytic cells, with small numbers of mature neutrophils and unique ultrastructural elements, such as nuclear pockets in the neutrophils, which accelerate the transport of RNA to the cytoplasm (20). Cells from hUCB have already been used to reconstitute bone marrow and blood cell lineages in children with various hematologic malignant and nonmalignant diseases after treatment with myeloablative doses of chemoradiotherapy (21)(22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Human Umbilical Cord Blood Cells Benefits an Animal Model of Sanfilippo Syndrome Type B

Garbuzova‐Davis

Willing

Desjarlais

et al. 2005

Stem Cells and Development

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Sanfilippo syndrome type B is caused by alpha-N-acetylglucosaminidase (Naglu) enzyme deficiency leading to an accumulation of undegraded heparan sulfate, a glycosaminoglycan (GAG). Cell therapy is a promising new treatment and human umbilical cord blood (hUCB) cell transplantation may be preferred for delivery of the missing enzyme. We investigated the ability of mononuclear hUCB cells administered into the lateral cerebral ventricle to ameliorate/prevent histopathological changes in mice modeling Sanfilippo syndrome type B. These are the first results supporting enzyme replacement by administered hUCB cells. In vivo, transplanted hUCB cells survived long-term (7 months), migrated into the parenchyma of the brain and peripheral organs, expressed neural antigens, and exhibited neuron and astrocyte-like morphology. Transplant benefits were also demonstrated by stable cytoarchitecture in the hippocampus and cerebellum, and by reduced GAGs in the livers of treated mutant mice. A hUCB cell transplant may be an effective therapeutic strategy for enzyme delivery in Sanfilippo syndrome type B.

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Ultrastructural and cytochemical characterization of human cord blood cells

Cited by 17 publications

References 14 publications

Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury

Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury

Nuclear envelope-limited chromatin sheets (ELCS) and heterochromatin higher order structure

Transplantation of Human Umbilical Cord Blood Cells Benefits an Animal Model of Sanfilippo Syndrome Type B

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