Ultrastructural Analysis on Lumbar Disc Herniation Using Surgical Specimens

Kobayashi, Shigeru; Meir, Adam; Kokubo, Yasuo; Uchida, Kenzo; Takeno, Kenichi; Miyazaki, Tsuyoshi; Yayama, Takafumi; Kubota, Masafumi; Nomura, Eiki; Mwaka, Erisa; Baba, H.

doi:10.1097/brs.0b013e31819c9d5b

Cited by 73 publications

(65 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, IVDs from patients with NCDH had significantly more neovascularization and granulation tissue than patients with CDH. This is consistent with results from previous studies (37,47), which showed that annulus fibrosus disruption could induce changes in the microenvironment surrounding herniated disc tissues. The present study evaluated the differences in neovascularization between the CDH and NCDH groups; however, no post-treatment follow-up evaluations were performed.…”

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

Role of interleukin-17 in chondrocytes of herniated intervertebral lumbar discs

Tian¹,

Li²,

Fu³

et al. 2015

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Lumbar disc herniation (LDH) is a common cause of lumbosacral radiculopathy. An autoimmune response to a herniated nucleus pulposus (NP) has been suggested to play an important role in the initiation of radiculopathy. Interleukin-17 (IL-17) is a cytokine associated with inflammation and autoimmunity. The presence of IL-17 has been studied in patients with LDH; however, extensive investigation into the expression of IL-17 in different disc pathologies of LDH has not yet been conducted. The aim of the present study was to investigate the role of neovascularization and hypertrophic chondrocytes in herniated intervertebral lumbar discs. Fifty-two intervertebral lumbar disc specimens were extracted from 46 patients with LDH and were subsequently classified as either contained or non-contained disc herniation (CDH and NCDH, respectively). The specimens were stained with hematoxylin and eosin or toluidine blue, or were immunostained with polyclonal antibodies to IL-17 using the streptavidin-peroxidase method. The neovascular tissue and staining results were graded to establish the histological differences between the two herniation types. The intervertebral discs (IVDs) obtained from patients with NCDH showed significantly more neovascularization and granulation tissue than the discs obtained from patients with CDH (P<0.05). Furthermore, hypertrophic chondrocytes were more abundant in the NCDH specimens than in the CDH specimens (P<0.05). Similarly, the number of IL-17-immunoreactive cells was significantly higher in the NCDH specimens than that in the CDH specimens (P<0.01).In conclusion, local inflammation and autoreactive immune activation may play an important role in the pathogenesis of LDH. These results also suggest a role of chondrocytes in the repair of herniated IVDs.

show abstract

Section: Discussionsupporting

confidence: 82%

“…Paraffin sections (5-µm-thick) were cut and stained with hematoxylin and eosin and toluidine blue. Surgical specimens were qualitatively graded according to whether or not inflammatory changes existed in the herniation (47) (Fig. 1) and hypertrophic chondrocytes were present in the specimens (Fig.…”

Section: Methodsmentioning

confidence: 99%

Role of interleukin-17 in chondrocytes of herniated intervertebral lumbar discs

Tian¹,

Li²,

Fu³

et al. 2015

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…An increase in expression of some cytokines and MMPs in herniated IVD may occur when molecules seen as 'non-self' by immune cells become exposed. This may also be linked to the phenomenon of spontaneous regression or disappearance of extruded IVD fragments, which has been attributed to matrix degradation and phagocytosis by recruited/infiltrated macrophages [102][103][104][105][106]. In cases with transligamentous extrusion, which can occur when the NP is potentially more exposed to immune cells, regression occurs [107].…”

Section: Mmp19mentioning

confidence: 99%

Inflammation in intervertebral disc degeneration and regeneration

Molinos

Almeida

Caldeira

et al. 2015

J. R. Soc. Interface.

327

256

View full text Add to dashboard Cite

Intervertebral disc (IVD) degeneration is one of the major causes of low back pain, a problem with a heavy economic burden, which has been increasing in prevalence as populations age. Deeper knowledge of the complex spatial and temporal orchestration of cellular interactions and extracellular matrix remodelling is critical to improve current IVD therapies, which have so far proved unsatisfactory. Inflammation has been correlated with degenerative disc disease but its role in discogenic pain and hernia regression remains controversial. The inflammatory response may be involved in the onset of disease, but it is also crucial in maintaining tissue homeostasis. Furthermore, if properly balanced it may contribute to tissue repair/regeneration as has already been demonstrated in other tissues. In this review, we focus on how inflammation has been associated with IVD degeneration by describing observational and in vitro studies as well as in vivo animal models. Finally, we provide an overview of IVD regenerative therapies that target key inflammatory players.

show abstract

“…The spontaneous resorption of prolapsed lumbar IVD tissue has frequently been observed [23-25] although the exact mechanisms by which this occur are not yet fully understood. Inflammatory and autoimmune responses provoked in the innate and adaptive immune systems on detection of displaced tissue likely play a role, with infiltrating monocytes [26], macrophages [26-29], T and B lymphocytes [27,28] and fibroblasts [29] reported in prolapsed NP tissue.…”

Section: Introductionmentioning

confidence: 99%

The cytokine and chemokine expression profile of nucleus pulposus cells: implications for degeneration and regeneration of the intervertebral disc

et al. 2013

View full text Add to dashboard Cite

IntroductionThe aims of these studies were to identify the cytokine and chemokine expression profile of nucleus pulposus (NP) cells and to determine the relationships between NP cell cytokine and chemokine production and the characteristic tissue changes seen during intervertebral disc (IVD) degeneration.MethodsReal-time q-PCR cDNA Low Density Array (LDA) was used to investigate the expression of 91 cytokine and chemokine associated genes in NP cells from degenerate human IVDs. Further real-time q-PCR was used to investigate 30 selected cytokine and chemokine associated genes in NP cells from non-degenerate and degenerate IVDs and those from IVDs with immune cell infiltrates (‘infiltrated’). Immunohistochemistry (IHC) was performed for four selected cytokines and chemokines to confirm and localize protein expression in human NP tissue samples.ResultsLDA identified the expression of numerous cytokine and chemokine associated genes including 15 novel cytokines and chemokines. Further q-PCR gene expression studies identified differential expression patterns in NP cells derived from non-degenerate, degenerate and infiltrated IVDs. IHC confirmed NP cells as a source of IL-16, CCL2, CCL7 and CXCL8 and that protein expression of CCL2, CCL7 and CXCL8 increases concordant with histological degenerative tissue changes.ConclusionsOur data indicates that NP cells are a source of cytokines and chemokines within the IVD and that these expression patterns are altered in IVD pathology. These findings may be important for the correct assessment of the ‘degenerate niche’ prior to autologous or allogeneic cell transplantation for biological therapy of the degenerate IVD.

show abstract

Ultrastructural Analysis on Lumbar Disc Herniation Using Surgical Specimens

Cited by 73 publications

References 34 publications

Role of interleukin-17 in chondrocytes of herniated intervertebral lumbar discs

Role of interleukin-17 in chondrocytes of herniated intervertebral lumbar discs

Inflammation in intervertebral disc degeneration and regeneration

The cytokine and chemokine expression profile of nucleus pulposus cells: implications for degeneration and regeneration of the intervertebral disc

Contact Info

Product

Resources

About