Ultrastructural alterations of the tegument of Raillietina echinobothrida treated with the stem bark of Acacia oxyphylla (Leguminosae)

Dasgupta, Shyamashree; Roy, Bishnupada; Tandon, Veena

doi:10.1016/j.jep.2009.10.017

Cited by 28 publications

(26 citation statements)

References 16 publications

(17 reference statements)

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“…In the present study, the tegumental surfaces of the test plant-treated parasites were found to be affected along with severe disruption of the underlying structures, as also observed earlier when treated with ethanolic crude extract of the plant by Dasgupta et al (2010). Similar to the present observations, destruction of absorptive surface was also caused by other drugs like praziquantel, oxyclozanide, and ethanol extract of several plants in trematodes and cestodes (Mehlhorn et al 1983;Tandon et al 1997;Roy et al 2008).…”

Section: Discussionsupporting

confidence: 88%

“…The genus also contains saponins like Acaciaside A and B which have been proven to have nematocidal and cestocidal activity (Ghosh et al 1993(Ghosh et al , 1996. Acacia oxyphylla, used widely against gastrointestinal worms in the rural traditional medicine practised among the Mizo natives in North-East India, has recently been reported as responsible for alteration of surface ultrastructure in cestode (Roy et al 2007;Dasgupta et al 2010). So, the aim of the present study was to examine any alterations brought about by this plant in the tegumental enzymes of Raillietina echinobothrida, a cestode parasite of domestic fowl.…”

Section: Introductionmentioning

confidence: 99%

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Antiparasitic activity of methanolic extract of Acacia oxyphylla (Leguminosae) against Raillietina echinobothrida

Dasgupta

Roy

2010

J Parasit Dis

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Acacia oxyphylla has been used traditionally by the natives of Mizoram against intestinal worm infections. In the present study, the crude methanolic extract of the plant was tested in vitro on the cestode parasite Raillietina echinobothrida to evaluate its potential anthelmintic efficacy. The test parasites were exposed to varying concentrations of the plant extract in phosphate buffered saline and they all slipped into a flaccid paralytic state at varying periods of time post incubation. Ultrastructural observations on the paralysed worms revealed wide scale destruction of the parasite tegument with intense vacuolization of the syncytium and swellings of the basal lamina accompanied by deformities in the cell organelles. To determine the exact mode of action of the botanicals on the parasite body surface, the tegumental enzymes viz. acid phosphatase (AcPase), alkaline phosphatase (AlkPase) and adenosine triphosphatase (ATPase) were investigated. A prominent decrease in the phosphatase activity, in comparison to the untreated control parasites was noticeable. In view of the alterations in the structural and functional integrity of the tegument, occurring in the treated parasites, it may be assumed that the changes transpire because of a compromise in the permeability of the tegument under the influence of the test plant-derived active chemical principles.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Antiparasitic activity of methanolic extract of Acacia oxyphylla (Leguminosae) against Raillietina echinobothrida

Dasgupta

Roy

2010

J Parasit Dis

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“…This is due to the fact that the tegumental and/or cuticular structures are the primary parasite-host interfaces, vital for absorption of nutrients and perception of the surrounding micro-environment provided by the host (William et al 2001;Mckinstry et al 2003;Rivera et al 2004;Xiao et al 2004;Roy et al 2008). Histological studies have revealed that anthelmintic agents induce considerable changes in the internal structural features of helminths, resulting in loss of normal cellular conformity in vital tissues (Roy et al 2008;Dasgupta et al 2010). In the present investigation, the plant extract was shown to cause reduction in the staining activity of the tegumental enzymes.…”

Section: Discussionmentioning

confidence: 99%

Potentilla fulgens (Family Rosaceae), a medicinal plant of north-east India: a natural anthelmintic?

et al. 2010

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The cestode parasite, Raillietina echinobothrida and the trematode, Gastrothylax crumenifer were exposed to the ethanolic root peel extract of Potentilla fulgens, an antiparasitic local medicinal plant of Meghalaya, India, to evaluate the anthelmintic efficacy of the plant. The parasites were incubated in 1, 5, 10, 20, 50 and 100 mg crude alcoholic extract per ml of phosphate buffered saline (PBS) at a temperature of 37 ± 1°C. Paralysis and death were observed at 2.00 ± 0.05 and 2.80 ± 0.06 h for the cestode and 1.21 ± 0.06 and 2.18 ± 0.04 h for the trematode parasites at the highest test concentration of the plant extract. The commercial anthelmintic, Praziquantel (PZQ) showed higher activity at the tested concentration (0.02 mg/ml). To further investigate the efficacy of the plant extract, vital tegumental enzymes of the parasite viz. Acid phosphatase (AcPase), Alkaline phosphatase (AlkPase) and Adenosine triphosphatase (ATPase) were studied. Quantitatively, the total enzyme activity of AcPase, AlkPase and ATPase was found to be reduced significantly by 69.20, 66.43 and 29.63% for R. echinobothrida and 47.96, 51.79 and 42.63% for G. crumenifer, respectively compared to the respective controls; histochemical study also showed reduction in the visible staining of the enzymes. The reference drug, PZQ also showed more or less similar effect like that of the plant extract. The result suggests that phytochemicals of P. fulgens have anthelmintic potential.

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“…Segundo os autores, a capacidade dos TC se ligarem às proteínas pode explicar as lesões observadas na cutícula pela microscopia eletrônica de varredura (MEV). Os efeitos anti-helmínticos das plantas ricas em TC podem ser atribuídos a diferentes mecanismos: redução na contagem de OPG, inibição da eclosão, inibição da alimentação larval, inibição do desenvolvimento larval, inibição da motilidade de L 3 e inibição da sobrevivência e redução na carga parasitária de NGI adultos (MINHO et al, 2008a;COSTA et al, 2008;MARTÍNEZ-ORTÍZ-DE-MONTELLANO et al, 2010;AZANDO et al, 2011;DASGUPTA;ROY;TANDON, 2010).…”

Section: Ação Direta Dos Tc Sobre O Parasitounclassified

Efeito anti-helmíntico de taninos condensados em nematódeos gastrintestinais de ovinos (Ovis aries)

2013

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ResumoAs helmintoses gastrintestinais constituem um dos principais fatores limitantes na produção de ovinos em todo o mundo. O desenvolvimento da resistência dos parasitos aos anti-helmínticos tem gerado grandes perdas na produção de pequenos ruminantes. Na busca por novas alternativas de controle efetivo de nematódeos gastrintestinais em pequenos ruminantes, a fitoterapia constitui um campo promissor de pesquisas na tentativa de reduzir a utilização de tratamentos químicos, visando uma produção agroecológica. Muitas plantas desempenham um papel crucial na manutenção da vida animal e humana. Durante a evolução humana muitas interações com outros organismos foram estabelecidas com consequências para ambas as partes de diversas maneiras. Plantas desenvolveram mecanismos bioquímicos para se defender de antagonistas biológicos que atuam como seus inimigos naturais. Este princípio tem levado os cientistas a procurar compostos bioativos produzidos pelas plantas contra os agentes patogénicos. Uma vez identificados, estes metabólitos são avaliados em relação a seu efeito sobre doenças de importância não só para a saúde pública, mas também na produção animal. O presente artigo apresenta uma revisão dos estudos realizados com plantas taniníferas cientificamente testadas em todo o mundo para o controle das parasitoses gastrintestinais em ruminantes. Palavras-chave: Fitoterapia, helmintos gastrintestinais, pequenos ruminantes, taninos AbstractGastrointestinal helminthiasis infections are considerate one of the main limiting factors in sheep production worldwide. The development of parasite resistance to anthelmintics has caused significant losses of small ruminant production. In the search for new alternatives for effective control of gastrointestinal nematodes in small ruminants, phytotherapy is a promising field of research in an attempt to reduce the usage of chemical treatments, aimed at ecological production. Many plants play a crucial role in maintaining animal and human life. During human evolution many interactions with other organisms were established and they have affected each other in many ways. Plants have developed biochemical mechanisms to defend themselves from biological antagonists that act as their natural enemies. This principle has led scientists to search for bio-active compounds produced by plants against pathogens. Since these discovers their metabolites are evaluated against diseases of importance not only in public health, but also in animal production. This article presents a review of studies with tanniniferous plants scientifically tested worldwide for the control of gastrointestinal parasites in ruminants.

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Ultrastructural alterations of the tegument of Raillietina echinobothrida treated with the stem bark of Acacia oxyphylla (Leguminosae)

Cited by 28 publications

References 16 publications

Antiparasitic activity of methanolic extract of Acacia oxyphylla (Leguminosae) against Raillietina echinobothrida

Antiparasitic activity of methanolic extract of Acacia oxyphylla (Leguminosae) against Raillietina echinobothrida

Potentilla fulgens (Family Rosaceae), a medicinal plant of north-east India: a natural anthelmintic?

Efeito anti-helmíntico de taninos condensados em nematódeos gastrintestinais de ovinos (Ovis aries)

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