Ultrastructural alterations inSaccharomyces cerevisiaecells in association with elevated temperature-induced autolysis

Abstract

By means of the freeze‐etching technique ultrastructural alterations in Saccharomyces cerevisiae cells undergoing autolysis at elevated temperature were studied. Wall surfaces of intact cells were smooth. During autolysis wall surfaces became rough with granules of 20–40 nm diameter. This alteration occurred after extensive disintegration of cytoplasmic organelles and after functional and ultrastructural impairments of the plasma membrane, but well before the rupture of the plasma membrane.

SaccharomycesYeast Cells Grown at Elevated Temperatures Are Susceptible to Autolysis

“…As mentioned above, structural alterations were hardly detectable either in cell wall or in cellular membranes after growth of yeast cells at elevated temperatures, in contrast to the previous observations 6 ) which found marked changes during incubation of the cells for autolysis. The intactness of cell structure during cultivation at elevated temperatures was consistent with the fact that few cellular materials were released from the cells before incubation for autolysis ( Fig.…”

“…The ultrastructural alterations in cell wall and in cellular membranes (the plasma membrane and intracellular membranes) were investigated with an electron microscope by freeze-etching technique as described previously. 6) Estimation of susceptibility of yeast cells to cell wall lytic enzyme. The reaction mixture (10 ml) contained about 60 mg dry wt.…”

Poliovirus 2Apro expression inhibits growth of yeast cells

“…2). This extensive desintegration of cytoplasmic organelles is equivalent to that described during autolysis of other fungi with micelial cells, but in yeast cells few ultrastructural studies about autolysis have been reported [23].…”

Cell Killing by HIV-1 Protease

“…However, the possibility that HIV-1 PR might be directly involved in cell wall degradation seems very unlikely. The cell wall affords mechanical protection to yeast cells against osmotic lysis (45,46). Indeed, growth of lytic strains with mutations in genes involved in cell wall integrity can be effectively restored by osmotic stabilizers (41).…”