Ultrasound of alternating frequencies and variable emotional impact evokes depressive syndrome in mice and rats

Morozova, Anastasia; Зубков, Э. А.; Strekalova, Tatyana; Кекелидзе, Зураб Ильич; Storozeva, Zinaida; Schroeter, Careen A.; Bazhenova, Nataliia; Lesch, Klaus‐Peter; Cline, Brandon H.; Chekhonin, V. P.

doi:10.1016/j.pnpbp.2016.03.003

Cited by 34 publications

(46 citation statements)

References 88 publications

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“…Duration of floating, determined as the absence of any directed movements of an animal's head and body, was video recorded and scored off-line during the last 6 min of the test, using a digital camera and RealTimer software (Open Science, Russia). Previous studies have validated the applied protocol with antidepressant treatment (Morozova et al, 2016).…”

Section: Forced Swim Testmentioning

confidence: 99%

Alteration of oxidative stress markers and behavior of rats in a novel model of depression

Gorlova¹,

Павлов²,

Зубков³

et al. 2019

Acta Neurobiologiae Experimentalis

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Emotional stress is considered a serious pathogenetic factor of depression. In this study an ultrasound model of emotional stress developed in our laboratory was applied. It is characterized by the use of ultrasound as the stressor agent. Animals are triggered not by any organic or physical disturbances but by the perception of adverse information. This type of stress can induce depressive-like behavioral changes in rodents, manifested by decreased sucrose preference and increased time of immobility in a forced swim test. Ultrasound stress also increased the levels of oxidative stress markers. This is important, as stress has an established association with increased oxidative processes in the central nervous system. Total glutathione and carbonyl protein content were selected as relevant brain markers, as glutathione plays a critical role in cellular defensive mechanisms during oxidative stress and the level of protein carbonyls can be a measure of global protein oxidation. We demonstrated that two weeks of chronic exposure to ultrasound was enough to cause depressive-like behavioral changes in rats. Increased levels of oxidative stress markers in the hippocampus and prefrontal cortex were also observed after two weeks of such stress. The current study has two goals: the first is to study the relationship of depression and oxidative stress; the second is an additional validation of our approach to modeling stress-induced depressive-like states in rats. The present data further support the validity of the ultrasound model by expanding information related to the influence of ultrasound stress on behavioral and physiological parameters, which are of great importance in the development of stress-induced depression. A time correlation between the onset of symptoms and a change in the level of oxidative stress markers in the brain is also demonstrated.

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Section: Forced Swim Testmentioning

confidence: 99%

Alteration of oxidative stress markers and behavior of rats in a novel model of depression

Gorlova¹,

Павлов²,

Зубков³

et al. 2019

Acta Neurobiologiae Experimentalis

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“…We additionally examined expression levels of brain derived neurotrophic factor ) BDNF ( , which has a role in the etiology of major depression and is a reliable biomarker for the depressed state 67 , 68 . Acute and chronic stress decreases BDNF expression levels in the hippocampus of rodents 69 - 71 , while various types of chronic antidepressant treatments increase BDNF expression in this region 72 , 73 . Using RT-qPCR, we found that both EAAT1 (Fig.…”

Section: Resultsmentioning

confidence: 98%

Therapeutic Effect of Astroglia-like Mesenchymal Stem Cells Expressing Glutamate Transporter in a Genetic Rat Model of Depression

et al. 2017

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Recent studies have proposed that abnormal glutamatergic neurotransmission and glial pathology play an important role in the etiology and manifestation of depression. It was postulated that restoration of normal glutamatergic transmission, by enhancing glutamate uptake, may have a beneficial effect on depression. We examined this hypothesis using unique human glial-like mesenchymal stem cells (MSCs), which in addition to inherent properties of migration to regions of injury and secretion of neurotrophic factors, were differentiated to express high levels of functional glutamate transporters (excitatory amino acid transporters; EAAT). Additionally, gold nanoparticles (GNPs), which serve as contrast agents for CT imaging, were loaded into the cells for non-invasive, real-time imaging and tracking of MSC migration and final location within the brain. MSC-EAAT (2×105; 10 μl) were administered (i.c.v.) to Flinder Sensitive Line rats (FSLs), a genetic model for depression, and longitudinal behavioral and molecular changes were monitored. FSL rats treated with MSC-EAAT showed attenuated depressive-like behaviors (measured by the forced swim test, novelty exploration test and sucrose self-administration paradigm), as compared to controls. CT imaging, Flame Atomic Absorption Spectroscopy analysis and immunohistochemistry showed that the majority of MSCs homed specifically to the dentate gyrus of the hippocampus, a region showing structural brain changes in depression, including loss of glial cells. mRNA and protein levels of EAAT1 and BDNF were significantly elevated in the hippocampus of MSC-EAAT-treated FSLs. Our findings indicate that MSC-EAATs effectively improve depressive-like manifestations, possibly in part by increasing both glutamate uptake and neurotropic factor secretion in the hippocampus.

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“…Moreover, increased sert gene expression in the prefrontal cortex, hippocampus, and midbrain were related to the development of depressive-like behavior in rats after prolonged exposure to ultrasonic waves [60]. Transgenic mice with 2- to 3-fold increases in sert gene expression in the raphe nuclei and a range of forebrain regions exhibited a low-anxiety phenotype [61].…”

Section: Discussionmentioning

confidence: 99%

Changes in Gene Expression Profiles in Adult Rat Brain Structures after Neonatal Action of Dipeptidyl Peptidase-IV Inhibitors

et al. 2017

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Background: Previous studies have shown the development of emotional and motivational disorders, such as anxiety-depression-like disorders with increased aggression in adolescent and adult Wistar rats, occurs after neonatal exposure to the dipeptidyl peptidase-IV (DPP-IV, EC 3.4.14.5) inhibitors diprotin A and sitagliptin (postnatal days 5–18). Methods: In this study, using real-time PCR, we evaluated changes in the gene expression of serine protease DPP-IV and prolyl endopeptidase (PREP, EC 3.4.21.26; dpp4 and prep genes), monoamine oxidase А (maoA) and B (maoB), and serotonin transporter (SERT; sert) in the brain structures from 3-month-old rats after postnatal action of DPP-IV inhibitors diprotin A and sitagliptin. Results: Dpp4, sert, and maoB gene expression increased and maoA gene expression changed with a tendency to increase in the striatum of rats with neonatal sitagliptin action. The increase of maoA gene expression was also shown in the amygdala. An increase in prep gene expression was found in the striatum of rats with the neonatal action of diprotin A, and a decrease in maoB gene expression was observed in the amygdala. We detected a significant downward trend in sert gene expression in the frontal cortex and amygdala, as well as a tendency to increase in maoA gene expression in the hypothalamus. Discussion: These findings suggest that changes in the expression of the abovementioned genes are associated with the development of anxiety and depression, with increased aggression caused by the neonatal action of diprotin A and sitagliptin.

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Ultrasound of alternating frequencies and variable emotional impact evokes depressive syndrome in mice and rats

Cited by 34 publications

References 88 publications

Alteration of oxidative stress markers and behavior of rats in a novel model of depression

Alteration of oxidative stress markers and behavior of rats in a novel model of depression

Therapeutic Effect of Astroglia-like Mesenchymal Stem Cells Expressing Glutamate Transporter in a Genetic Rat Model of Depression

Changes in Gene Expression Profiles in Adult Rat Brain Structures after Neonatal Action of Dipeptidyl Peptidase-IV Inhibitors

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