Ultrasound microbubble contrast agent-mediated suicide gene transfection in the treatment of hepatic cancer

Tang, Qing; He, Xinrong; Liao, Haixing; He, Lian-tu; Wang, Ying; Zhou, Dansha; Ye, Sumin; Chen, Qilu

doi:10.3892/ol.2012.845

Cited by 14 publications

(12 citation statements)

References 9 publications

(7 reference statements)

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“…A method to enhance the local treatment was investigated by Tang Q et al [51], for a human hepatic cancer cell line model intratumoral administration of KDR-TK and AFP-TK with microbubble contrast agent prior to ultrasound treatment. The pro-drugs 5-FC and GCV were administered after the intratumoral therapy.…”

Section: Discussionmentioning

confidence: 99%

“…Additional control of micrometastases, through the efficient local control of microenvironment, has also been achieved with suicide gene therapy [49]. Finally, suicide gene therapy has been explored by using the state of the art technology of nanoparticles, either as a mode to passively transport the suicide gene formulation, or as a mode to efficiently display the diffusion of the formulation [43,50,51]. …”

Section: Introductionmentioning

confidence: 99%

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Suicide Gene Therapy for Cancer; Old Dog New Tricks

Zarogoulidis

Darwiche

Sakkas³

et al. 2013

J Genet Syndr Gene Ther

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Current cancer treatments may create profound iatrogenic outcomes. The adverse effects of these treatments still remain, as the serious problems that practicing physicians have to cope with in clinical practice. Although, non-specific cytotoxic agents constitute an effective treatment modality against cancer cells, they also tend to kill normal, quickly dividing cells. On the other hand, therapies targeting the genome of the tumors are both under investigation, and some others are already streamlined to clinical practice. Several approaches have been investigated in order to find a treatment targeting the cancer cells, while not affecting the normal cells. Suicide gene therapy is a therapeutic strategy, in which cell suicide inducing transgenes are introduced into cancer cells. The two major suicide gene therapeutic strategies currently pursued are: cytosine deaminase/5-fluorocytosine and the herpes simplex virus/ganciclovir. The novel strategies include silencing gene expression, expression of intracellular antibodies blocking cells’ vital pathways, and transgenic expression of caspases and DNases. We analyze various elements of cancer cells’ suicide inducing strategies including: targets, vectors, and mechanisms. These strategies have been extensively investigated in various types of cancers, while exploring multiple delivery routes including viruses, non-viral vectors, liposomes, nanoparticles, and stem cells. We discuss various stages of streamlining of the suicide gene therapy into clinical oncology as applied to different types of cancer. Moreover, suicide gene therapy is in the center of attention as a strategy preventing cancer from developing in patients participating in the clinical trials of regenerative medicine. In oncology, these clinical trials are aimed at regenerating, with the aid of stem cells, of the patients’ organs damaged by pathologic and/or iatrogenic factors. However, the stem cells carry the risk of neoplasmic transformation. We discuss cell suicide inducing strategies aimed at preventing stem cell-originated cancerogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Suicide Gene Therapy for Cancer; Old Dog New Tricks

Zarogoulidis

Darwiche

Sakkas³

et al. 2013

J Genet Syndr Gene Ther

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“…Zhoa et al 2012 insonated breast cancer cells in the presence of microbubbles and doxorubicin-loaded liposomes; they reported that ultrasound mediated cavitation lead to the observed reduction in cell viability in the treated cell cultures. In an in vivo study, Lin et al (2012[a]) used polyethylene glycol coated liposomes loaded with doxorubicin to treat a murine colorectal adenocarcinoma implanted subcutaneously in mice.…”

Section: Ultrasound Mediated Chemotherapymentioning

confidence: 99%

“…On some occasions the genetic material and microbubbles were injected separately into a tumor (Tang et al 2012) or alternatively they were mixed and then added to the tissue culture or injected into the tumor (Li et al 2012[c]; Li et al 2014; Sakakima et al 2005; Yamaguchi et al 2011[a]; Zhang et al 2011; Zolochevska et al 2011). An improved distribution throughout the parenchyma of the tumor would, however, be expected when the nucleotide and microbubble are linked and injected intravenously.…”

Section: Ultrasound Mediated Gene Transfectionmentioning

confidence: 99%

“…In these ultrasound-mediated methods, the transfection/transduction of genetic material was usually accomplished using non-viral techniques, but using a virus as a carrier with microbubbles was also successful (Li et al 2014; Tang et al 2012). Compared with viral vectors, non viral techniques have increased safety but are disadvantaged by low transfection efficiencies (Wang et al 2012[c]), however, microbubbles are good carriers of genes with a greater capacity for antisense oligonucleotides, and fragment of DNA and even the entire chromosome (Li et al 2012[c]).…”

Section: Ultrasound Mediated Gene Transfectionmentioning

confidence: 99%

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A Review of Low-Intensity Ultrasound for Cancer Therapy

Wood

Sehgal

2015

Ultrasound in Medicine & Biology

280

230

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The literature describing the use of low-intensity ultrasound in four major areas of cancer therapy was reviewed - sonodynamic therapy, ultrasound mediated chemotherapy, ultrasound mediated gene delivery and antivascular ultrasound therapy. Each technique consistently resulted in the death of cancer cells and the bioeffects of ultrasound were primarily attributed to thermal actions and inertial cavitation. In each therapeutic modality, theranostic contrast agents composed of microbubbles played a role in both therapy and vascular imaging. The development of these agents is important as it establishes a therapeutic-diagnostic platform which can monitor the success of anti-cancer therapy. Little attention, however, has been given to either the direct assessment of the underlying mechanisms of the observed bioeffects or to the viability of these therapies in naturally occurring cancers in larger mammals; if such investigations provided encouraging data there could be a prompt application of a therapy technique in treating cancer patients.

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Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of VEGFR2-targeted CD-TK-loaded cationic nanobubbles in the treatment of bladder cancer

Jiang

et al. 2020

J Cancer Res Clin Oncol

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Ultrasound microbubble contrast agent-mediated suicide gene transfection in the treatment of hepatic cancer

Cited by 14 publications

References 9 publications

Suicide Gene Therapy for Cancer; Old Dog New Tricks

Suicide Gene Therapy for Cancer; Old Dog New Tricks

A Review of Low-Intensity Ultrasound for Cancer Therapy

Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of VEGFR2-targeted CD-TK-loaded cationic nanobubbles in the treatment of bladder cancer

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