Ultrasound-mediated microbubble destruction facilitates gene transfection in rat C6 glioma cells

Wang, Junfeng; Wu, Changjun; Zhang, Chunmei; Qiu, Qian-yi; Zheng, Miao

doi:10.1007/s11033-008-9307-3

Cited by 16 publications

(10 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our experiment showed that TMB assisted gene delivery with ultrasound mediation was able to achieve a gene transfection efficiency of around 43.9%, which was significantly higher than that of the other treatment groups. This result is consistent with previous reports [31,34–36]. Interestingly, TMB assisted gene delivery with ultrasound mediation also yielded a significantly higher reporter plasmid expression in comparison with gene delivery with NMBs or without microbubbles.…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells

Chang

Guo

Sun

et al. 2013

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

Ultrasound-targeted microbubble destruction (UTMD) technique can be potentially used for non-viral delivery of gene therapy. Targeting wild-type p53 (wtp53) tumor suppressor gene may provide a clinically promising treatment for patients with ovarian cancer. However, UTMD mediated gene therapy typically uses non-targeted microbubbles with suboptimal gene transfection efficiency. We synthesized a targeted microbubble agent for UTMD mediated wtp53 gene therapy in ovarian cancer cells. Lipid micro-bubbles were conjugated with a Luteinizing Hormone–Releasing Hormone analog (LHRHa) via an avidin– biotin linkage to target the ovarian cancer A2780/DDP cells that express LHRH receptors. The microbubbles were mixed with the pEGFP-N1-wtp53 plasmid. Upon exposure to 1 MHz pulsed ultrasound beam (0.5 W/cm2) for 30 s, the wtp53 gene was transfected to the ovarian cancer cells. The transfection efficiency was (43.90 ± 6.19)%. The expression of wtp53 mRNA after transfection was (97.08 ± 12.18)%. The cell apoptosis rate after gene therapy was (39.67 ± 5.95)%. In comparison with the other treatment groups, ultrasound mediation of targeted microbubbles yielded higher transfection efficiency and higher cell apoptosis rate (p < 0.05). Our experiment verifies the hypothesis that ultrasound mediation of targeted microbubbles will enhance the gene transfection efficiency in ovarian cancer cells.

show abstract

Section: Discussionsupporting

confidence: 93%

“…More recently, the emergence of gene therapy has further stimulated the microbubble research for the enhancement of gene delivery efficiency [28–31]. The ultrasound-targeted microbubble destruction (UTMD) technique has been developed to facilitate the increased regional uptake of plasmid DNA [11–13].…”

Section: Discussionmentioning

confidence: 99%

Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells

Chang

Guo

Sun

et al. 2013

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

show abstract

“…In previous studies, ultrasound frequencies of 1-3 MHz have been used (11,35). However, the present study used low-frequency (21 kHz) and low-energy ultrasound, which is associate with easy penetration of the organism, less tissue absorption and reduced tissue injury.…”

Section: Discussionmentioning

confidence: 99%

Liposome-mediated transfection of wild-type P53 DNA into human prostate cancer cells is improved by low-frequency ultrasound combined with microbubbles

Bai

Zhang

et al. 2016

Oncology Letters

View full text Add to dashboard Cite

Abstract. Prostate cancer is a common type of cancer in elderly men. The aim of the present study was to evaluate the effects of ultrasound exposure in combination with SonoVue microbubbles on liposome-mediated transfection of wild-type P53 genes into human prostate cancer cells. PC-3 human prostate cancer cells were exposed to ultrasound; duty cycle was controlled at 20% (2 sec on, 8 sec off) for 5 min with and without SonoVue microbubble echo-contrast agent using a digital sonifier (frequency, 21 kHz; intensity, 46 mW/cm 2 ). The cells were divided into eight groups, as follows: Group A (SonoVue + wild-type P53), group B (ultrasound + wild-type P53), group C (SonoVue + ultrasound + wild-type P53), group D (liposome + wild-type P53), group E (liposome + SonoVue + wild-type P53), group F (liposome + wild-type P53 + ultrasound), group G (liposome + wild-type P53 + ultrasound + SonoVue) and the control group (wild-type P53). Following treatment, a hemocytometer was used to measure cell lysis, reverse transcription-quantitative polymerase chain reaction and western blotting were performed to detect P53 gene transfection efficiency, Cell Counting Kit-8 was employed to reveal cell proliferation and Annexin V/propidium iodide staining was used to determine cell apoptosis. Cell lysis was minimal in each group. Wild-type P53 gene and protein expression were significantly increased in the PC-3 cells in group G compared with the control and all other groups (P<0.01). Cell proliferation was significantly suppressed in group G compared with the control group and all other groups (P<0.01). Cell apoptosis levels in group G were significantly improved compared with the control group and all other groups (P<0.01). Thus, the results of the present study indicate that the use of low-frequency and low-energy ultrasound in combination with SonoVue microbubbles may be a potent physical method for increasing liposome gene delivery efficiency.

show abstract

“…Research analyzing the longitudinal effects of antiangiogenic gene therapy on hepatocellular carcinoma revealed a significant decrease in micro-vessel density and increase in apoptosis using US-stimulated therapy with plasmid compared with plasmid alone (Nie et al 2008). Various other studies have investigated US-stimulated delivery of genetic material in the heart (Bekeredjian et al 2005; Shohet et al 2000; Tsunoda et al 2005), pancreas (Chen et al 2006), skeletal muscle (Wang et al 2005; Zhang et al 2006), kidney (Koike et al 2005), central nervous system (Shimamura et al 2005) and solid tumors (Nie et al 2008; Wang et al 2009; Warram et al 2012). …”

Section: Discussionmentioning

confidence: 99%

Enhancement of Adenovirus Delivery after Ultrasound-Stimulated Therapy in a Cancer Model

Sorace¹,

Warram²,

Mahoney³

et al. 2013

Ultrasound in Medicine & Biology

View full text Add to dashboard Cite

Improving the efficiency of adenovirus (Ad) delivery to target tissues has the potential to advance the translation of cancer gene therapy. Ultrasound (US)-stimulated therapy uses microbubbles (MBs) exposed to low-intensity US energy to improve localized delivery. We hypothesize that US-stimulated gene therapy can improve Ad infection in a primary prostate tumor through enhanced tumor uptake and retention of the Ad vector. In vitro studies were performed to analyze the degree of Ad infectivity after application of US-stimulated gene therapy. A luciferase-based Ad on a ubiquitous cytomegalovirus (CMV) promoter (Ad5/3-CMV-Luc) was used in an animal model of prostate cancer (bilateral tumor growth) to evaluate Ad transduction efficiency after US-stimulated therapy. Bioluminescence imaging was employed for In vivo analysis to quantify Ad infection within the tumor. In vitro studies revealed no difference in Ad transduction between groups receiving US-stimulated therapy using high, low or sham US intensity exposures at various multiplicities of infection (MOIs) (p = 0.80). In vivo results indicated that tumors receiving US-stimulated therapy after intra-tumoral injection of Ad5/3-CMV-Luc (1 × 106 plaque-forming units) exhibited a 95.1% enhancement in tumor delivery compared with control tumors receiving sham US (p = 0.03). US-stimulated therapy has significant potential to immediately affect Ad-based cancer gene therapy by improving virus bioavailability in target tissues.

show abstract

Ultrasound-mediated microbubble destruction facilitates gene transfection in rat C6 glioma cells

Cited by 16 publications

References 20 publications

Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells

Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells

Liposome-mediated transfection of wild-type P53 DNA into human prostate cancer cells is improved by low-frequency ultrasound combined with microbubbles

Enhancement of Adenovirus Delivery after Ultrasound-Stimulated Therapy in a Cancer Model

Contact Info

Product

Resources

About