Ultrasound-mediated intracellular drug delivery using microbubbles and temperature-sensitive liposomes

Yudina, Anna; Smet, de M Mariska; Lepetit‐Coiffé, Matthieu; Langereis, Sander; Ruijssevelt, van S; Смирнов, П. Р.; Bouchaud, Véronique; Voisin, Pierre; Grüll, Holger; Moonen, C.

doi:10.1016/j.jconrel.2011.06.006

Cited by 74 publications

(40 citation statements)

References 31 publications

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“…This could be due to the internalized PI which became more fluorescent after nuclear uptake and complexation with genetic material. In contrast, Yudina et al demonstrated that the cellular uptake of cell-impermeable small compounds persisted up to 24 hours with a half-life of 8 hours [84]. Since the internalization of these sompounds persist beyond the pore lifetimes, other phenomena beyond the pores themselves must contribute to the drug uptake.…”

Section: Spatio-temporal Aspects Of Inertial Cavitationmentioning

confidence: 97%

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Lentacker

Cock

Deckers

et al. 2014

Advanced Drug Delivery Reviews

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In the past two decades, research has underlined the potential of ultrasound and microbubbles to enhance drug delivery. However, there is less consensus on the biophysical and biological mechanisms leading to this enhanced delivery. Sonoporation, i.e. the formation of temporary pores in the cell membrane, as well as enhanced endocytosis is reported. Because of the variety of ultrasound settings used -and corresponding microbubble behavior, a clear overview is missing. Therefore, in this review, the mechanisms contributing to sonoporation are categorized according to three ultrasound settings: i) low intensity ultrasound leading to stable cavitation of microbubbles, ii) high intensity ultrasound leading to inertial cavitation with microbubble collapse, and iii) ultrasound application in the absence of microbubbles. Using low intensity ultrasound, the endocytotic uptake of several drugs could be stimulated, while short but intense ultrasound pulses can be applied to induce pore formation and the direct cytoplasmic uptake of drugs. Ultrasound intensities may be adapted to create pore sizes correlating with drug size. Small molecules are able to diffuse passively through small pores created by low intensity ultrasound treatment. However, delivery of larger drugs such as nanoparticles and gene complexes, will require higher ultrasound intensities in order to allow direct cytoplasmic entry.2

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Section: Spatio-temporal Aspects Of Inertial Cavitationmentioning

confidence: 97%

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Lentacker

Cock

Deckers

et al. 2014

Advanced Drug Delivery Reviews

Self Cite

644

592

View full text Add to dashboard Cite

show abstract

“…Triggered-release approaches based on molecular motors are another area of considerable interest, and have enjoyed a favorable regulatory opinion. For instance, a number of systems have combined external energy sources such as pH, ultrasound, heat or light (Yudina et al, 2011;Bibi et al, 2012) with appropriate lipid compositions in order to improve controlled drug release at the tumor site. They have, however, been largely disappointing in practice, mostly due to difficult engineering.…”

Section: Introductionmentioning

confidence: 99%

Pharmaceutical liposomal drug delivery: a review of new delivery systems and a look at the regulatory landscape

2016

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Liposomes were the first nanoscale drug to be approved for clinical use in 1995. Since then, the technology has grown considerably, and pioneering recent work in liposome-based delivery systems has brought about remarkable developments with significant clinical implications. This includes long-circulating liposomes, stimuli-responsive liposomes, nebulized liposomes, elastic liposomes for topical, oral and transdermal delivery and covalent lipid-drug complexes for improved drug plasma membrane crossing and targeting to specific organelles. While the regulatory bodies' opinion on liposomes is well-documented, current guidance that address new delivery systems are not. This review describes, in depth, the current state-of-the-art of these new liposomal delivery systems and provides a critical overview of the current regulatory landscape surrounding commercialization efforts of higher-level complexity systems, the expected requirements and the hurdles faced by companies seeking to bring novel liposomebased systems for clinical use to market.

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“…This is beneficial for drugs when they are penetrating cells. Research in which TO-PRO-3-loaded thermosensitive liposomes and microbubbles released TO-PRO-3 in response to heating with high intensity focused ultrasound 113 could be even more advantageous to sonoporate the target tissue followed by administration of drug-loaded ultrasound contrast agents.…”

Section: Permeabilization Of Cell Membranes For Drug Transportmentioning

confidence: 99%

Potential and problems in ultrasound-responsive drug delivery systems

Zhao¹,

Du²,

Lu³

et al. 2013

IJN

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Abstract:Ultrasound is an important local stimulus for triggering drug release at the target tissue. Ultrasound-responsive drug delivery systems (URDDS) have become an important research focus in targeted therapy. URDDS include many different formulations, such as microbubbles, nanobubbles, nanodroplets, liposomes, emulsions, and micelles. Drugs that can be loaded into URDDS include small molecules, biomacromolecules, and inorganic substances. Fields of clinical application include anticancer therapy, treatment of ischemic myocardium, induction of an immune response, cartilage tissue engineering, transdermal drug delivery, treatment of Huntington's disease, thrombolysis, and disruption of the blood-brain barrier. This review focuses on recent advances in URDDS, and discusses their formulations, clinical application, and problems, as well as a perspective on their potential use in the future.

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Ultrasound-mediated intracellular drug delivery using microbubbles and temperature-sensitive liposomes

Cited by 74 publications

References 31 publications

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms

Pharmaceutical liposomal drug delivery: a review of new delivery systems and a look at the regulatory landscape

Potential and problems in ultrasound-responsive drug delivery systems

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