Ultrasound-induced uptake of different size markers in mammalian cells

Karshafian, Raffi; Bevan, Peter D.; Burns, Peter N.; Samac, Sanya; Banerjee, Manidipa

doi:10.1109/ultsym.2005.1602785

Cited by 19 publications

(42 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sonoporation-induced cellular uptake of markers with molecular weights between 10 kDa and 2 MDa has been reported in several studies [17,96]. Schlicher et al showed that ultrasoundinduced cavitation facilitated cellular uptake of macromolecules with diameters up to 56 nm [118].…”

Section: Discussionmentioning

confidence: 84%

“…Even solid spheres with a 100 nm diameter have been successfully delivered by sonoporation [25]. This implies that drug size is not a limiting factor for intracellular delivery [96]. However, the pore opening times can be so short, that, if the drug is to be effectively internalized, it should be released close to the cell membrane when poration occurs [97].…”

Section: Discussionmentioning

confidence: 99%

“…In frame 9, a 5 m droplet is left inside the gas microbubble, while the protrusion retracts. Karshafian et al found, that contrast microbubbles insonified near a cell at any acoustic pressure used cause large pores (300-500 nm), whereas microbubbles insonified at high acoustic pressures cause smaller pores (20-500 nm), too, in more cells [96]. Pores on the order of 10-100 nm were observed with two different methods by [97], with pores opening lasting only milliseconds to seconds.…”

Section: What Causes Sonoporation?mentioning

confidence: 99%

“…The two contrast agents used in [96] have mean diameters 1.1-3.3 and 2.0-4.5 m, respectively [34]. The lower and upper limit of these diameters has been modeled and shown in Fig.…”

Section: What Causes Sonoporation?mentioning

confidence: 99%

See 3 more Smart Citations

Ultrasound-Directed Drug Delivery

Postema¹,

Gilja²

2007

CPB

View full text Add to dashboard Cite

It has been proven, that the cellular uptake of drugs and genes is increased, when the region of interest is under ultrasound insonification, and even more when a contrast agent is present. This increased uptake has been attributed to the formation of transient porosities in the cell membrane, which are big enough for the transport of drugs into the cell (sonoporation). Owing to this technique, new ultrasound contrast agents that incorporate a therapeutic compound have become of interest. Combining ultrasound contrast agents with therapeutic substances, such a chemotherapeutics and virus vectors, may lead to a simple and economic method to instantly cure upon diagnosis, using conventional ultrasound scanners. There are two hypotheses for explaining the sonoporation phenomenon, the first being microbubble oscillations near a cell membrane, the second being microbubble jetting through the cell membrane. Based on modeling, high-speed photography, and recent cellular uptake measurements, it is concluded that microbubble jetting behavior is less likely to be the dominant sonoporation mechanism. Ultrasound-directed drug delivery using microbubbles is a promising method that has great potential in the treatment of malignant disorders.

show abstract

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: What Causes Sonoporation?mentioning

confidence: 99%

“…The two contrast agents used in [96] have mean diameters 1.1-3.3 and 2.0-4.5 m, respectively [34]. The lower and upper limit of these diameters has been modeled and shown in Fig.…”

Section: What Causes Sonoporation?mentioning

confidence: 99%

See 2 more Smart Citations

Ultrasound-Directed Drug Delivery

Postema¹,

Gilja²

2007

CPB

View full text Add to dashboard Cite

show abstract

“…This allows the non-specific uptake of molecules like fluorescent dyes, genes or drugs [2][3][4]. Large scale oscillation of nearby microbubbles (MBs) is considered to be the primary effect in sonoporation whereas MB destruction correlates with lower sonoporation efficiency [5] and increased cell damage [6].…”

Section: Abstract-ultrasound Contrast Agents Sonoporation Therapy mentioning

confidence: 99%

Evaluation of subharmonic emission from encapsulated microbubbles as an indicator for sonoporation of cell monolayers

Hensel

Haagen

Schmitz

et al. 2009

2009 IEEE International Ultrasonics Symposium

View full text Add to dashboard Cite

Sonoporation is the ultrasound induced transient opening of cell membranes. Large scale oscillation of nearby microbubbles (MBs) is considered to be the primary effect in sonoporation whereas MB destruction correlates with lower sonoporation efficiency and increased cell damage. The scale of MB oscillation in a cloud can be determined by monitoring subharmonic emission. In this study, SW480 cells are grown in Opticell containers, which are filled with a growth medium containing SonoVue MBs and propidium iodide (PI). Each container is placed in water in the focus of a single element transducer emitting 10 cycles sine-bursts at 3.3 MHz. The peak negative pressure is varied from 75 to 750 kPa. Scattered signals are recorded by a broadband transducer to monitor subharmonic-to-fundamental ratio (SFR) during therapy. A fluorescence microscope is focused on the therapy region to monitor PI fluorescence during and after acoustic excitation. PI cannot overcome intact cell membranes and changes its fluorescence properties when bound to intracellular fluid. A cell is considered sonoporated, if it reveals a sharp rise of fluorescence intensity and a subsequent decrease. A cell is considered permanently stained, if fluorescence intensity is concentrated at the cell nucleus and rises to a plateau. For maximum peak negative excitation pressure, maximum sonoporation (4.9%) and maximum permanent poration (2.8%) is observed. Correcting the number of sonoporated cells by the number of permanently stained cells, best sonoporation efficiency is achieved for the amplitude of 520 kPa. The same holds for the SFR, which significantly rises to a maximum of -26,6 dB at 520 kPa, too. The relationship of sonoporation efficiency and SFR reveals that subharmonic emission from ultrasound contrast agent is an indicator for sonoporation efficiency of cell monolayers.

show abstract