Ultrasound induced production of thrombinase by marine actinomycetes: Kinetic and optimization studies

Naveena, Balakrishnan; Sakthiselvan, Punniavan; Elaiyaraju, Periyasamy; Partha, Nagarajan

doi:10.1016/j.bej.2011.12.007

Cited by 32 publications

(18 citation statements)

References 25 publications

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“…The ultrasonic stimulation was supplied using the ''duty cycle'' control, which consisted of 10 s working time and 10 s stop time. The intermittent ultrasound has been used to ultrasonic treatment of microorganism in order to reduce the formation of free radicals [20,21]. The mycelial pellets in flasks were exposed to one 5 min ultrasonic treatment at different growth stages (day 1-6) in the shake flask culture.…”

Section: Ultrasonic Treatmentmentioning

confidence: 99%

Ultrasound-intensified laccase production from Trametes versicolor

Wang

Chen

et al. 2013

Ultrasonics Sonochemistry

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a b s t r a c tAn efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the highest extracellular laccase activity of 588.9 U/L among all treatments tested which was 1.8-fold greater than the control without ultrasound treatment. The ultrasonic treatment resulted in a higher pellet porosity that facilitated the mass transfer of nutrients and metabolites from the pellets to the surrounding liquid. Furthermore, the ultrasonic treatment induced the expression of the laccase gene (lcc), which correlated with a sharp increase in both extracellular and intracellular laccase activity. This is the first study to find positive effects of ultrasound on gene expression in fungal cells. These results provide a basis for understanding the stimulation of metabolite production and process intensification by ultrasonic treatment in filamentous fungal culture.

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Section: Ultrasonic Treatmentmentioning

confidence: 99%

Ultrasound-intensified laccase production from Trametes versicolor

Wang

Chen

et al. 2013

Ultrasonics Sonochemistry

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“…The cumulative bio‐hydrogen production from the optimized carbon source was analyzed using the modified Gompertz equation :

B_{normalt} = B \exp true(- \exp true(\frac{R_{normalb} e}{B} (λ - t) + 1 true) true)

where B t is the cumulative bio‐hydrogen production (mL) at any time ( t ); B is the bio‐hydrogen production (mL); R b is the maximum bio‐hydrogen production rate (mL/day); e = 2.718; λ is the lag phase (days), which is the minimum time taken to produce bio‐hydrogen or time taken by fungi to acclimatize to the environment. The specific bio‐hydrogen production (mL H 2 /g VS) and specific bio‐hydrogen production rate (mL H 2 /day per g VS) was acquired by dividing B and R b , respectively, by gram VS of sludge.…”

Section: Methodsmentioning

confidence: 99%

Bio‐hydrogen Production By Enzymic Hydrolysis of Sunflower Oil Sludge

Sakthiselvan

Naveena

Gopinath³

et al. 2015

CLEAN Soil Air Water

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Bio‐hydrogen was produced from different agro‐industrial wastes by Hypocrea lixii SS1 isolated from fouled soil. The alkaline pre‐treatment of sunflower oil sludge resulted in a bio‐hydrogen potential of 405 mL H2/g volatile solids (VS). The role of sunflower oil sludge on the bio‐hydrogen production by H. lixii SS1 was investigated. It was inferred that xylose enhances the bio‐hydrogen production when compared to other monomeric carbohydrates in sunflower oil sludge. A maximum bio‐hydrogen potential of 430 mL H2/g VS was achieved with 7.2 mg/mL of xylose formation for a 168 h incubation period. The bio‐hydrogen production was carried out under optimized conditions of 80 g/L inoculum, pH 5 and 50 °C, and a maximum bio‐hydrogen potential of 494.26 mL H2/g VS was obtained. A cumulative bio‐hydrogen production of 450 mL was achieved with modified Gompertz model. The maximum chemical oxygen demand (COD) removal efficiency (93%) and maximum fermentative products (2182.60 mg COD/L) with total volatile fatty acids of 2032.82 mg COD/L were obtained when 120 and 80 g/L of inoculum were added to the reactor, respectively. Hence, enzymatic hydrolysis led to a marked improvement in the overall bio‐hydrogen yield of 400–500 mL H2/g VS suggesting the potential use of sunflower oil sludge for bio‐hydrogen production in a cost effective manner.

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“…According to some recent reports, the enormous pool of the diversity present in the marine ecosystems could provide a potential natural reservoir for useful biocatalysts (Naveena et al, 2012;Senthil et al, 2012). The novel chemical and stereochemical characteristics of the marine biocatalysts would add to their worth.…”

Section: The Enzymes From the Marine Actinomycetesmentioning

confidence: 98%