Ultrasound-Induced Expression of a Heat Shock Promoter-Driven Transgene Delivered in the Kidney by Genetically Modified Mesenchymal Stem Cells

Letavernier, Béatrice; Salomir, Rarès; Delmas, Yahsou; Rome, Claire; Couillaud, Franck; Desmoulière, Alexis; Dubus, Isabelle; Moreau‐Gaudry, François; Grosset, Christophe; Hauger, Olivier; Rosenbaum, Jean; Grenier, N.; Combe, Christian; Ripoche, Jean; Moonen, Chrit

doi:10.3109/9781420019933-15

Cited by 2 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mesenchymal stem cells (MSC) obtained from bone marrow of rats were transfected with hsp-luc expression of the luciferase gene under the control of an hsp70 promoter. The transformed MSCs were injected in the left renal artery and transgenic expression was induced by MRI-controlled HIFU hyperthermia (59). Figure 3 shows a summary of the histologic findings.…”

Section: Applications Of Local Temperature Elevation In Gene Therapymentioning

confidence: 99%

Spatio-Temporal Control of Gene Expression and Cancer Treatment Using Magnetic Resonance Imaging–Guided Focused Ultrasound

Moonen

2007

Clinical Cancer Research

View full text Add to dashboard Cite

Local temperature elevation may be used for tumor ablation, gene expression, drug activation, and gene and/or drug delivery. High-intensity focused ultrasound (HIFU) is the only clinically viable technology that can be used to achieve a local temperature increase deep inside the human body in a noninvasive way. Magnetic resonance imaging (MRI) guidance of the procedure allows in situ target definition and identification of nearby healthy tissue to be spared. In addition, MRI can be used to provide continuous temperature mapping during HIFU for spatial and temporal control of the heating procedure and prediction of the final lesion based on the received thermal dose. The primary purpose of the development of MRI-guided HIFU was to achieve safe noninvasive tissue ablation.The technique has been tested extensively in preclinical studies and is now accepted in the clinic for ablation of uterine fibroids. MRI-guided HIFU for ablation shows conceptual similarities with radiation therapy. However, thermal damage generally shows threshold-like behavior, with necrosis above the critical thermal dose and full recovery below. MRI-guided HIFU is being clinically evaluated in the cancer field.The technology also shows great promise for a variety of advanced therapeutic methods, such as gene therapy. MR-guided HIFU, together with the use of a temperature-sensitive promoter, provides local, physical, and spatiotemporal control of transgene expression. Specially designed contrast agents, together with the combined use of MRI and ultrasound, may be used for local gene and drug delivery.

show abstract

Section: Applications Of Local Temperature Elevation In Gene Therapymentioning

confidence: 99%

Spatio-Temporal Control of Gene Expression and Cancer Treatment Using Magnetic Resonance Imaging–Guided Focused Ultrasound

Moonen

2007

Clinical Cancer Research

View full text Add to dashboard Cite

show abstract

The Use of Ultrasound in Transfection and Transgene Expression

Rome¹,

Deckers²,

Moonen³

2008

Molecular Imaging II

View full text Add to dashboard Cite

The interaction of ultrasound with tissue leads to radiation pressure, heat generation, and cavitation. These phenomena have been utilised for local gene delivery, transfection and control of expression. Specially designed nanocarriers or adapted ultrasound contrast agents can further enhance local delivery by: (1) increased permeability of cell membranes; (2) local release of genes. Biological carriers may also be used for local gene delivery. Stem cells and immune cells appear especially promising because of their homing capabilities to lesion sites. Imaging methods can be employed for pharmacodistribution and pharmacokinetics. MRI contrast agents can serve as non-invasive reporters on gene distribution when co-delivered with the gene. They can be used to label nanocarriers and cellular transport systems in gene therapy strategies such as those based on stem cells. Finally, ultrasound heating together with the use of a temperature sensitive promoter allows a local, physical, spatio-temporal control of transgene expression, in particular when combined with MRI temperature mapping for monitoring and even controlling ultrasound heating.

show abstract

Ultrasound-Induced Expression of a Heat Shock Promoter-Driven Transgene Delivered in the Kidney by Genetically Modified Mesenchymal Stem Cells

Cited by 2 publications

References 19 publications

Spatio-Temporal Control of Gene Expression and Cancer Treatment Using Magnetic Resonance Imaging–Guided Focused Ultrasound

Spatio-Temporal Control of Gene Expression and Cancer Treatment Using Magnetic Resonance Imaging–Guided Focused Ultrasound

The Use of Ultrasound in Transfection and Transgene Expression

Contact Info

Product

Resources

About