Ultrasound improved ethanol fermentation from cassava chips in cassava-based ethanol plants

Nitayavardhana, Saoharit; Shrestha, Prachand; Rasmussen, Mary L.; Lamsal, Buddhi P.; Leeuwen, J. van; Khanal, Samir Kumar

doi:10.1016/j.biortech.2009.10.075

Cited by 87 publications

(55 citation statements)

References 9 publications

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“…However, because the starch granules in cassava are large and complex structures, a high reactor loading of enzyme is required. To overcome this problem, on-going eVorts to optimize the STARGEN-based SSF processing of raw substrate are the focus of current experimental work [17,22]. On the other hand, on-site production of multiactivity enzyme preparations from Penicillium sp.…”

Section: Introductionmentioning

confidence: 99%

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Sugimoto

Makita

Watanabe

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Cassava is a starch-containing root crop that is widely used as a raw material in a variety of industrial applications, most recently in the production of fuel ethanol. In the present study, ethanol production from raw (uncooked) cassava flour by simultaneous saccharification and fermentation (SSF) using a preparation consisting of multiple enzyme activities from Aspergillus kawachii FS005 was investigated. The multi-activity preparation was obtained from a novel submerged fermentation broth of A. kawachii FS005 grown on unmilled crude barley as a carbon source. The preparation was found to consist of glucoamylase, acid-stable α-amylase, acid carboxypeptidase, acid protease, cellulase and xylanase activities, and exhibited glucose and free amino nitrogen (FAN) production rates of 37.7 and 118.7 mg/l/h, respectively, during A. kawachii FS005-mediated saccharification of uncooked raw cassava flour. Ethanol production from 18.2% (w/v) dry uncooked solids of raw cassava flour by SSF with the multi-activity enzyme preparation yielded 9.0% (v/v) of ethanol and 92.3% fermentation efficiency. A feasibility study for ethanol production by SSF with a two-step mash using raw cassava flour and the multi-activity enzyme preparation manufactured on-site was verified on a pilot plant scale. The enzyme preparation obtained from the A. kawachii FS005 culture broth exhibited glucose and FAN production rates of 41.1 and 135.5 mg/l/h, respectively. SSF performed in a mash volume of about 1,612 l containing 20.6% (w/v) dry raw cassava solids and 106 l of on-site manufactured A. kawachii FS005 culture broth yielded 10.3% (v/v) ethanol and a fermentation efficiency of 92.7%.

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Section: Introductionmentioning

confidence: 99%

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Sugimoto

Makita

Watanabe

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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“…Cassava plants are grown in drought condition to improve the economy of the country because of its high anthocyanin content and protein for animal feed 4 . cassava starches are an excellent raw material for food industry to modify the physical properties of many foods for example as gelling, thickening, adhesion, moisture retention, stabilizing, texturizing and anti staling applications 5 .The cassava leaves contain lot of anti nutrients, such as tannins, oxalate, phytate and trypsin inhibitors 6 . Cassava leaves shows high profiling of minerals particularly calcium and trace minerals 7 .…”

Section: Introductionmentioning

confidence: 99%

Nutritional and Anti Nutritional Constituents of Manihot Esculentus and Plecutranthus Rotundifolius

Anbuselvi¹,

Balamurugan²

2013

Int. Res. J. Pharm.

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The nutritional and anti nutritional characterization of two tubers namely Manihot esculenta and Plecutranthus rotundifolius were analyzed. A comparative analysis of nutrients of cassava and Chinese potato were carried out using standard analytical techniques. Results obtained showed high amount of protein, vitamins and low amount of phosphorus were found in leaves of chinese potato. Cassava pulp revealed 65 mg of calcium and 28.6 mg of vitamin A. 29 mg of reducing sugar and 1.8 mg of phosphorus were found in cassava leaves. Anti nutrients were accumulated more in cassava than chinese potato.

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“…Nitayavardhana et al [39] reported that a considerable particle size reduction of cassava chips resulted in a significant improvement in the sugar yield by 180 % during enzymatic hydrolysis. Nitayavardhana et al [26] also found that the US pretreatment of cassava chip slurries enhanced the overall ethanol yield and fermentation rate. The ethanol yield from the sonicated sample increased by 2.7-fold while the fermentation time was reduced by nearly 24 h for sonicated samples to achieve the same ethanol yield as control sample.…”

Section: Ultrasonic Destruction and Fractionation Of Lignocellulosic mentioning

confidence: 95%

“…US generates monolithic cavitation and results in physical and chemical reactions in liquid solutions. Ultrasonic irradiation of various types of biomass including maize [33], cassava chip slurry [26], corn meal [34], switchgrass [35], sugarcane bagasse [36], and waxy rice [37] have shown that US enhances the rate of enzymatic hydrolysis and bioethanol production. Luo et al [38] critically discussed the application of US technology in the biomass pretreatment intended for biorefinery and biofuel applications and highlighted the process benefits of its application, including shorter treatment time and lower energy requirements, and increased accessibility to enzymatic hydrolysis and delignification.…”

Section: Ultrasonic Destruction and Fractionation Of Lignocellulosic mentioning

confidence: 99%

“…Compared to microwave, gamma-ray, and electron beam, US is the most studied irradiation method. The application of US in the field of biorenewables is still fairly a new concept, and has demonstrated potential as a facile processing technique to enhance the enzymatic hydrolysis and subsequent ethanol fermentation [25,26]. The application of US facilitates mass transfer in addition to the turbulent mixing and secondary effects attributed to acoustic cavitation (the formation, growth, and collapse of bubbles) [27].…”

Section: Introductionmentioning

confidence: 98%

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Ultrasound as a Green Processing Technology for Pretreatment and Conversion of Biomass into Biofuels

Tang

Sivakumar

2014

Production of Biofuels and Chemicals With Ultrasound

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In the past decades, a great deal of attention has been focused on mechanical, thermal or chemical pretreatments of lignocellulosic and algal biomass for the production of biofuel. This chapter is focused on the potential of ultrasound (US) technology in pretreating the biomass to enhance the conversion of cellulose to fermentable sugars and also the disintegration and component extraction of microalgae for the generation of bioethanol or biogas. In addition, US can supplement existing biomass pretreatment methods to greatly enhance their performance and their efficacy. Low frequency ultrasound (LFU, 20-100 kHz) is commonly used in biomass processing, particularly in processes that require intense physical effects such as cell disruption and polymer degradation. High frequency ultrasound (HFU, 400 kHz-2 MHz) recently attracted considerable interest as potential alternative technique for pretreating both the lignocellulosic and algal biomass for sustainable biofuel production. HFU is gaining increasing importance because of its environmentally sound and energy-saving production method since it demands lower energy input for the conversion of biomass. It not only saves time and energy but also lowers the chemical/enzyme dosage and hence novel and considered to be a new sustainable and environmentally-friendly green technique. Although many studies have shown the promise of ultrasound in the cell breakdown for enhanced enzymatic hydrolysis, there is limited information in the application of HFU on biomass pretreatment processes. This chapter provides an overview on the fundamentals of US, the critical parameters that control the conversion of biomass, challenges involved with the application of US in the biomass conversion and its future perspectives.

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Ultrasound improved ethanol fermentation from cassava chips in cassava-based ethanol plants

Cited by 87 publications

References 9 publications

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Production of multiple extracellular enzyme activities by novel submerged culture of Aspergillus kawachii for ethanol production from raw cassava flour

Nutritional and Anti Nutritional Constituents of Manihot Esculentus and Plecutranthus Rotundifolius

Ultrasound as a Green Processing Technology for Pretreatment and Conversion of Biomass into Biofuels

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