Ultrasound-guided percutaneous implantation of rabbit VX2 carcinoma, using a coaxial technique and gelfoam pellet injection combination to establish a rabbit liver tumor model

Cheng, Zhimei; Zhang, Shuai; Wang, Lizhou; Huang, Zhi; Wang, Ping; Zhu, Hong; Wei, Zijing; Zhou, Shi

doi:10.5152/dir.2021.20533

Cited by 3 publications

(4 citation statements)

References 16 publications

(21 reference statements)

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“…The rabbit model of the hepatic VX2 tumor was established according to the literature ( 14 , 15 ). All rabbits were anesthetized with 0.1 mL/kg of intramuscular injections of xylazine hydrochloride (Dunhua Shengda Animal Medicine Co., Ltd., Qingdao, China), along with 0.1 mL/kg of tiletamine hydrochlorid and zolazepam hydrochloride (Virbac Inc., Carros, France).…”

Section: Methodsmentioning

confidence: 99%

Precise localization of microvascular invasion in hepatocellular carcinoma based on three-dimensional histology-MR image fusion: an ex vivo experimental study

Li,

Chen,

Huang

et al. 2023

Quant Imaging Med Surg

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Background Microvascular invasion (MVI) is an independent risk factor for postoperative recurrence of hepatocellular carcinoma (HCC). However, MVI cannot be detected by conventional imaging. To localize MVI precisely on magnetic resonance (MR) images, we evaluated the feasibility and accuracy of 3-dimensional (3D) histology-MR image fusion of the liver. Methods Animal models of VX2 liver tumors were established in 10 New Zealand white rabbits under ultrasonographic guidance. The whole liver lobe containing the VX2 tumor was extracted and divided into 4 specimens, for a total of 40 specimens. MR images were obtained with a T2-weighted sequence for each specimen, and then histological images were obtained by intermittent, serial pathological sections. 3D histology-MR image fusion was performed via landmark registration in 3D Slicer software. We calculated the success rate and registration errors of image fusion, and then we located the MVI on MR images. Regarding influencing factors, we evaluated the uniformity of tissue thickness after sampling and the uniformity of tissue shrinkage after dehydration. Results The VX2 liver tumor model was successfully established in the 10 rabbits. The incidence of MVI was 80% (8/10). 3D histology-MR image fusion was successfully performed in the 39 specimens, and the success rate was 97.5% (39/40). The average registration error was 0.44±0.15 mm. MVI was detected in 20 of the 39 successfully registered specimens, resulting in a total of 166 MVI lesions. The specific location of all MVI lesions was accurately identified on MR images using 3D histology-MR image fusion. All MVI lesions showed as slightly hyperintense on the high-resolution MR T2-weighted images. The results of the influencing factor assessment showed that the tissue thickness was uniform after sampling (P=0.38), but the rates of the tissue shrinkage was inconsistent after dehydration (P<0.001). Conclusions 3D histology-MR image fusion of the isolated liver tumor model is feasible and accurate and allows for the successful identification of the specific location of MVI on MR images.

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Section: Methodsmentioning

confidence: 99%

Precise localization of microvascular invasion in hepatocellular carcinoma based on three-dimensional histology-MR image fusion: an ex vivo experimental study

Li,

Chen,

Huang

et al. 2023

Quant Imaging Med Surg

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“…Once necrotic tissue, fascia, and connective tissue had been removed, the tumor was cut into pieces sized approximately 0.5–1.0 mm 3 , and these pieces were placed in the outer sheath of an 18G blood vessel puncture needle (Hueli Medical Equipment Co., Wenzhou, China). The needle was then placed on ice, following a procedure described previously ( Cheng et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…For the procedure, anesthesia was induced and maintained using inhalant isoflurane (5%) (Shenzhen Ryvind Life Science & Technology Co. Guangdong, China) in oxygen (2.5%–3%) via a nose cone. The shallow probe of a portable bedside ultrasound machine (M6Vet, Shenzhen Mindray Bio-Medical Electronics Co., Guangdong, China) was used to guide implantation via percutaneous puncture as described in a previous publication ( Cheng et al, 2022 ). Through this procedure, 20 rabbits were implanted with rabbit VX2 liver tumor in the left lobe of the liver.…”

Section: Methodsmentioning

confidence: 99%

Intravoxel incoherent motion diffusion-weighted imaging and shear wave elastography for evaluating peritumoral liver fibrosis after transarterial chemoembolization in a VX2 rabbit liver tumor model

Cheng

Yang

et al. 2022

Front. Physiol.

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In this study, we sought to evaluate changes in peritumoral fibrosis after transarterial chemoembolization (TACE) in a rabbit VX2 liver tumor model using intravoxel incoherent motion diffusion-weighted imaging (IVIM DWI) and ultrasound shear wave elastography (SWE). A total of 20 rabbits underwent implantation of VX2 tumor tissues in the left lobe of the liver. The rabbits were randomly divided into an experimental group (n = 10) or a control group (n = 10). Those in the experimental group were treated with an emulsion of lipiodol and pirarubicin through a microcatheter 2–3 weeks after implantation; those in the control group were treated with sterile water. Compared with the control group, the true diffusion coefficient (D) and pseudodiffusion coefficient (D*) values in liver tissues were significantly lower (p < 0.05 for all) and liver stiffness values (LSV) (10.58 ± 0.89 kPa) were higher in the experimental group (7.65 ± 0.86 kPa; p < 0.001). The median stage of liver fibrosis based on METAVIR scores was 1 (1,1) in the control group and 2 (2,3) in the experimental group (Z = 4.15, p < 0.001). D, D*, and LSV were significantly correlated with pathologic staining in the assessment of liver fibrosis (r = −0.54 p = 0.015; r = −0.50, p = 0.025; r = 0.91, p < 0.001; respectively). These data suggest that TACE aggravates liver injury and liver fibrosis, especially surrounding the tumor, in a rabbit VX2 liver tumor model. IVIM DWI and SWE can be used to evaluate the change in liver fibrosis.

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“…VX2 tumor tissues purchased from Guangzhou Gineo Biotechnology Co. (Guangzhou, Guangdong, China) were implanted into the muscle layer of the hindlimb of one carrier rabbit (n = 1) and were grown for 2 to 3 weeks as described in previous study (17). The tumor tissues harvested from the carrier rabbit were then divided and subsequently implanted into the left liver lobe of the remaining rabbits (n = 15) under ultrasound guidance (M6Vet, Shenzhen Mindray Bio-Medical Electronics Co., Guangdong, China) via percutaneous puncture.…”

Section: Implantation Of Vx2 Liver Tumormentioning

confidence: 99%

Intravoxel incoherent motion imaging used to assess tumor microvascular changes after transarterial chemoembolization in a rabbit VX2 liver tumor model

et al. 2023

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PurposeTo evaluate the correlation between microvascular density (MVD) and intravoxel incoherent motion (IVIM) magnetic resonance imaging (MRI) parameters and the effect of glycolytic flux after transarterial chemoembolization (TACE) in a rabbit VX2 liver tumor.Materials and methodsVX2 liver tumor allografts in 15 New Zealand white rabbits were treated with sterile saline (control group, n = 5) or lipiodol-doxorubicin emulsion (experimental group, n = 10). MRI was performed 2 weeks after the procedure to evaluate IVIM parameters, including apparent diffusion coefficient (ADC), pure diffusion coefficient (D), pseudodiffusion coefficient (D*), and perfusion fraction (PF). All animal samples were taken of the tumor and surrounding liver. Immunostaining for CD31, CD34, CD105, and VEGF was used to evaluate MVD. The protein expression of Glut4, HK2, PKM2, LDHA, and MCT1 was determined using western blotting. Pearson correlation tests were used to analyze the relationship between MVD and IVIM parameters.ResultsD* value in the peritumoral region was negatively correlated with CD34 (r = –0.71, P = 0.01). PF value positively correlated with CD34 (r = 0.68, P = 0.015), CD105 (r = 0.76, P = 0.004) and VEGF (r = 0.72, P = 0.008) in the peritumoral region. Glut4, HK2, PKM2, and MCT1 in the peritumoral regions were higher in the experimental group than in the control group (all P < 0.05).ConclusionIVIM parameters were correlated with MVD in the intratumoral and peritumoral regions after TACE in a rabbit liver tumor model. The angiogenesis reflected by MVD may be related to changes of glycolytic flux.

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Ultrasound-guided percutaneous implantation of rabbit VX2 carcinoma, using a coaxial technique and gelfoam pellet injection combination to establish a rabbit liver tumor model

Abstract: et al. Ultrasound-guided percutaneous implantation of rabbit VX2 carcinoma, using a coaxial technique and gelfoam pellet injection combination to establish a rabbit liver tumor model.

Cited by 3 publications

References 16 publications

Precise localization of microvascular invasion in hepatocellular carcinoma based on three-dimensional histology-MR image fusion: an ex vivo experimental study

Precise localization of microvascular invasion in hepatocellular carcinoma based on three-dimensional histology-MR image fusion: an ex vivo experimental study

Intravoxel incoherent motion diffusion-weighted imaging and shear wave elastography for evaluating peritumoral liver fibrosis after transarterial chemoembolization in a VX2 rabbit liver tumor model

Intravoxel incoherent motion imaging used to assess tumor microvascular changes after transarterial chemoembolization in a rabbit VX2 liver tumor model

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