Aims/hypothesis Hypoglycaemia is associated with reduced skin temperature (Ts). We studied whether infrared thermography can detect Ts changes during hypoglycaemia in patients with type 1 diabetes and how the Ts response differs between patients with normal hypoglycaemia awareness and hypoglycaemia unawareness. Methods Twenty-four patients with type 1 diabetes (ten aware, 14 unaware) were studied during normoglycaemia (5.0-6.0 mmol/l), hypoglycaemia (2.0-2.5 mmol/l) and during recovery from hypoglycaemia (5.0-6.0 mmol/l) using hyperinsulinaemic glucose clamping. During each 1 h phase, Ts was measured twice by infrared thermography imaging in pre-defined areas (nose, glabella and the five left fingertips), symptoms of hypoglycaemia were scored and blood was sampled.Results Ts decreased during hypoglycaemia on the nose and glabella. The highest decrements were recorded on the nose (aware: −2.6°C, unaware: −1.1°C). In aware patients, the differences in temperature were statistically significant on both nose and glabella, whereas there was only a trend in the unaware group. There was a significant difference in hypoglycaemia-induced temperature changes between the groups. Patients in the aware group had higher hypoglycaemia symptom scores and higher adrenaline (epinephrine) levels during hypoglycaemia. Conclusions/interpretation The hypoglycaemia-associated decrement in Ts can be assessed by infrared thermography Diabetologia (2015) 58:1898-1906 DOI 10.1007 Electronic supplementary material The online version of this article (doi:10.1007/s00125-015-3616-6) contains peer-reviewed but unedited supplementary material, which is available to authorised users. and is larger in patients with normal hypoglycaemia awareness compared with unaware patients.