Ultrasound-guided injections of amniotic membrane/umbilical cord particulate for painful neuropathy of the lower extremity

Buksh, Ahmed Bilal

doi:10.1080/2331205x.2020.1724067

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…(6) Interestingly, FLO appears to relieve pain effectively in several ocular surface disorders, (7)(8)(9) and musculoskeletal disorders such as osteoarthritis (10,11) and lower extremity neuropathy. (12) However, the mechanisms underlying its potential pain inhibition properties and how it may affect sensory neuron excitability is also unknown.…”

Section: Introductionmentioning

confidence: 99%

Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain

Zhang,

Huang,

Ford

et al. 2024

Preprint

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Pain after surgery causes significant suffering. Opioid analgesics cause severe side effects and accidental death. Therefore, there is an urgent need to develop non-opioid therapies for managing post-surgical pain and, more importantly, preventing its transition to a chronic state. In a mouse model of post-surgical pain, local application of Clarix Flo (FLO), a human amniotic membrane (AM) product, attenuated established post-surgical pain hypersensitivity without exhibiting known side effects of opioid use in mice. Importantly, preemptive drug treatment also inhibited the transition of post-surgical pain to a prolonged state. This effect was achieved through direct inhibition of nociceptive dorsal root ganglion (DRG) neurons via CD44-dependent pathways, and indirect pain relief by attenuating immune cell recruitment. We further purified the major matrix component, the heavy chain-hyaluronic acid/pentraxin 3 (HC-HA/PTX3) from human AM that has greater purity and water solubility than FLO. HC-HA/PTX3 replicated FLO-induced neuronal and pain inhibition. Mechanistically, HC-HA/PTX3 induced cytoskeleton rearrangements to inhibit sodium current and high-voltage activated calcium current on nociceptive neurons, suggesting it is a key bioactive component mediating pain relief. Collectively, our findings highlight the potential of naturally derived biologics from human birth tissues as an effective non-opioid treatment for post-surgical pain and unravel the underlying mechanisms.

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