Ultrasound enhanced production of mycelia and exopolysaccharide by Agaricus bitorquis (Quél.) Sacc. Chaidam

Resveratrol, a natural polyphenol compound with multiple bioactivities, is widely used in food and pharmaceutical industry. Endophytic fungus Alternaria sp. MG1, as a native producer of resveratrol, shows increasing potential application. However, strategies for improvement of the biosynthesis of resveratrol in this species are still scarce. In this study, different elicitors were used to investigate their effect on the biosynthesis of resveratrol in MG1 and the induction mechanism. Ultrasound and sodium butyrate had no effect and slight inhibition on the resveratrol production and related gene expression, respectively. UV radiation and co-culture with Phomopsis sp. XP-8 significantly promoted the biosynthesis of resveratrol with the highest production (240.57μg/l) coming from UV 20min. Co-culture altered the profiles of secondary metabolites in MG1 by promoting and inhibiting the synthesis of stilbene and lignin compounds, respectively, and generating new flavonoids ((+/−)-taxifolin, naringin, and (+)-catechin). Oligomeric proanthocyanidins (OPC) also showed an obviously positive influence, leading to an increase in resveratrol production by 10 to 60%. Two calcium-dependent protein kinases (CDPK) were identified, of which CDPK1 was found to be an important regulatory factor of OPC induction. Synergistic treatment of UV 20min and 100μm OPC increased the production of resveratrol by 70.37% compared to control and finally reached 276.31μg/l.

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“…Sacc. Chaidam ( Lu et al, 2020 ). In the current study, ultrasound exposure (40kHz) for 10min caused no significant increase of resveratrol.…”

Section: Resultsmentioning

confidence: 99%

Improvement of the Biosynthesis of Resveratrol in Endophytic Fungus (Alternaria sp. MG1) by the Synergistic Effect of UV Light and Oligomeric Proanthocyanidins

Shi

Zhao

et al. 2021

Front. Microbiol.

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“…Twostage agitation speed control enhanced the triterpene production rate in Ganoderma lucidum by 49.22% (Feng et al, 2015). High pressure, electric fields, and ultrasound have been recently applied in microbial fermentation and could be used in macrofungus fermentation (Lu et al, 2020;Mota et al, 2018). Low-frequency magnetic fields and ultrasonic treatments affect mycelial growth and the biosynthesis of active substances (Gao, Zhang, & Feng, 2011;Zhang et al, 2014).…”

Section: Extrinsic Factorsmentioning

confidence: 99%

Macrofungi: A review of cultivation strategies, bioactivity, and application of mushrooms

Lou

et al. 2020

Comp Rev Food Sci Food Safe

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112

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The production of macrofungi (mushrooms) as well as their economic value have been steadily increasing globally. The use of functional foods, dietary supplements, and traditional medicines derived from macrofungi is increasing as they have numerous health benefits as well as abundant nutrients. Macrofungi are diverse with complex and highly varied growth conditions and bioactive constituents, most macrofungal resources have not yet been fully explored and applicated, leading to an urgent need for appropriate strategies to address the problem. Increasing attention has been paid to the macrofungal cultivation and application, in particular, potential prebiotics. Herein, the present review comprehensively summarizes recent progress in the cultivation, newly identified bioactive constituents, and their effects on gut microbiota as well as the potential ways in which they affect human health. Moreover, the macrofungal food development is discussed to improve food nutritional value and change the quality characteristics of food. Finally, the review addresses consumer safety concerns and the prospective genetic manipulation of macrofungi. We hope that this review can provide a comprehensive research reference for ensuring the safety and efficacy, along with maximizing the value and profitability of macrofungi production.

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“…Unlike the high intensity ultrasound, low intensity ultrasound is able to accelerate the proliferation of microbial cells, leading to enhancement of the products' metabolism [23,24,78]. It has frequently been suggested that the effect of ultrasound increases fluid convection and transport of molecules through the boundary layer of the liquid surrounding the cells [26,38,79]. Table 2 lists key examples of ultrasound applications to induce bioprocess productivity.…”

Section: Application Of Ultrasound For Improving Productivity Of Micrmentioning

confidence: 99%

Exploitation of Ultrasound Technique for Enhancement of Microbial Metabolites Production

et al. 2020

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Microbial metabolites have significant impacts on our lives from providing valuable compounds for nutrition to agriculture and healthcare. Ever-growing demand for these natural compounds has led to the need for smart and efficient production techniques. Ultrasound is a multi-applicable technology widely exploited in a range of industries such as chemical, medical, biotechnological, pharmaceutical, and food processes. Depending on the type of ultrasound employed, it can be used to either monitor or drive fermentation processes. Ultrasonication can improve bioproduct productivity via intensifying the performance of living organisms. Controlled ultrasonication can influence the metabolites’ biosynthesis efficiency and growth rates by improvement of cell permeability as well as mass transfer and nutrient uptake rates through cell membranes. This review contains a summarized description about suitable microbial metabolites and the applications of ultrasound technique for enhancement of the production of these metabolites as well as the associated downstream processing.

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Ultrasound enhanced production of mycelia and exopolysaccharide by Agaricus bitorquis (Quél.) Sacc. Chaidam

Cited by 17 publications

References 44 publications

Improvement of the Biosynthesis of Resveratrol in Endophytic Fungus (Alternaria sp. MG1) by the Synergistic Effect of UV Light and Oligomeric Proanthocyanidins

Improvement of the Biosynthesis of Resveratrol in Endophytic Fungus (Alternaria sp. MG1) by the Synergistic Effect of UV Light and Oligomeric Proanthocyanidins

Macrofungi: A review of cultivation strategies, bioactivity, and application of mushrooms

Exploitation of Ultrasound Technique for Enhancement of Microbial Metabolites Production

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