Objectives-To investigate the brain tissue elasticity in normal term and premature neonates using compression elastography and shear wave elastography.Methods-This prospective observational study enrolled term and premature neonates admitted to the Third Affiliated Hospital of Guangzhou Medical University between July 2019 and December 2020.Results-A total of 106 neonates, including 65 premature neonates and 41 term neonates, were enrolled. The elastic modulus of the frontal white matter in males was significantly lower than in females (11.67 AE 0.98 versus 12.25 AE 1.31, P = .030), but the shear wave velocity of the thalamus in males was significantly lower than in females (1.18 AE 0.13 versus 1.82 AE 0.10, P < .001). There was no significant correlation between real-time body weight and brain tissue elasticity including elastic modulus and shear wave velocity. But, the shear wave velocity of parietal white matter (r = 0.319, P = .014) and thalamus (r = À0.268, P = .040) and the elastic modulus of parietal white matter (r = 0.356, P = .006) were correlated with corrected gestational age.Conclusions-Clinicians may consider using elastography to determine brain tissue elasticity in term and preterm neonates. Key Words-brain; elasticity imaging techniques; neonates; technology; ultrasonography E arly detection and intervention to avoid brain damage and cognitive dysfunction in neonates with neurological diseases such as cerebral palsy are of the utmost importance. 1 Organ stiffness, including the brain's, can be a marker for the etiology and prognosis of various pathologies. [2][3][4] Clinically, elastography technologies are widely used for detecting lesions and tissue properties of the liver, kidney, thyroid, and lymph nodes in pediatric patients, especially for liver diseases such as liver fibrosis and biliary atresia. 3,4 Currently, imaging methods such as ultrasound elastography, magnetic resonance elastography (MRE), and computed tomography are widely used to determine the mechanical properties of brain tissues. [5][6][7] As a noninvasive, safe, radiation-free, low-cost, and reproducible examination