Ultrasound Effect on Gene Expression of Sex Determining Region Y-box 9 (SOX9) and Transforming Growth Factor β Isoforms in Adipose Stem Cells

Shafaei, Hajar; Baghernezhad, Hassan

doi:10.17795/zjrms-6465

Cited by 3 publications

(2 citation statements)

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“…This type of collagen gives tensile strength and provides most of the mechanical properties of cartilage tissue‏. 21 , 34 Decreased level of collagen type II by chondrocytes in monolayer condition after 6 days can be contributed to the loss of phenotype of this cells by increasing the time. Cartilage Oligomeric Matrix Protein (COMP) is a non-collagenous protein found in territorial matrix surrounding the chondrocytes and regulates the collagen network in cartilage tissue‏.…”

Section: Discussionmentioning

confidence: 99%

Conditioned Medium of Wharton's Jelly Derived Stem Cells Can Enhance the Cartilage Specific Genes Expression by Chondrocytes in Monolayer and Mass Culture Systems

2017

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Purpose: Mesenchymal stem cells (MSCs) have been introduced for cell therapy strategies in osteoarthritis (OA). Despite of their capacity for differentiation into chondrocyte, there are some evidences about their life-threatening problem after transplantation. So, some researchers shifted on the application of stem cells conditioned medium. The goal of this study is to evaluate whether Wharton's jelly derived stem cell conditioned medium (WJSCs-CM) can enhance the gene expression profile by chondrocytes in monolayer and mass culture systems. Methods: Conditioned medium was obtained from WJSCs at fourth passage. Isolated chondrocytes were plated at density of 1×106 for both monolayer and high density culture. Then cells in both groups were divided into control (received medium) and experiment group treated with WJ-CM for 3 and 6 days. Samples were prepared to evaluate gene expression profile of collagen II, aggrecan, cartilage oligomeric matrix protein (COMP) and sox-9 using real-time RT-PCR. Results: After 3 days, Chondrocytes treated with WJSCs-CM expressed significantly higher level of genes compared to the control group in both culture systems. After 6 days, the expression of genes in monolayer cultivated chondrocytes was decreased but that of the mass culture were up-regulated significantly. Conclusion: WJ-SCs-CM can increase the expression of cartilage-specific genes and can be introduced as a promoting factor for cartilage regeneration.

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Section: Discussionmentioning

confidence: 99%

Conditioned Medium of Wharton's Jelly Derived Stem Cells Can Enhance the Cartilage Specific Genes Expression by Chondrocytes in Monolayer and Mass Culture Systems

2017

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“…In fact it seems that LIUS differentiated constructs are in early stage of chondrogenesis than TGF constructs. It is possible ultrasound decreases hypertrophic markers of chondrogenesis by decreasing of TGFβ1 and TGFβ2 [50].…”

Section: Discussionmentioning

confidence: 99%

Effects of Low Intensity Ultrasound on the Chondrogenic Differentiation of Adult Stem Cells From Adipose Tissue

Shafaei

Esmaeili

Mardani

et al. 2016

Zahedan J Res Med Sci

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Background: Adult stem cells from adipose tissue can be used in tissue engineering because of their capacity to differentiate into chondrocytes. Low intensity ultrasound (LIUS) as a physical chondrogenic inducer differentiates adipose stem cells (ASC) into chondrocyte the same as transforming growth factor-β (TGFβ). However the stage of differentiation and hypertrophy of chondrocytes by LIUS have not yet been studied. Objectives: The aim of this study was to determine the effect of LIUS on hypertrophic states of differentiated chondrocytes. Materials and Methods: In this experimental study, ASCs were cultured in chondrogenic differentiation medium (10 ng/mL of TGFβ) with or without LIUS stimulation for two weeks. The ultrasound signal was applied at an intensity of 200 mW/cm 2 for 10

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Effect of low-intensity pulsed ultrasound on regenerative potential of transplanted ASCs â€“PCL construct in articular cartilage defects in sheep

Vahedi¹,

Roshangar²,

Jarolmasjed³

et al. 2016

Indian J of Anim Sci

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Articular cartilage is affected by weight loading and mechanical stimuli. Low intensity ultrasound promotes chondrogenesis in cartilage injury. This study was designed to show the effect of low-intensity pulsed ultrasound on chondrogenesis potential of transplanted adipose derived stem cells- polycaprolactone (ASCs â€“PCL) construct in vivo.The adipose tissue was obtained from infrapatellar fat pad of 5 male sheep. Adipose tissue derived stem cells (ASCs) at passage 2 were seeded in polycaprolactone (PCL). The cartilage defects were created on both sides of distal femoral articular cartilage. The right joint was chosen as control group. The left joint was chosen as experimental group and was exposed to low-intensity pulsed ultrasound with intensity 200 mW/cmÂ², 10 min/day for 6 weeks. After 6 months, animals were euthanized to retrieve repaired articular cartilage tissue. Macroscopic appearance of defects was examined and samples of repaired cartilage tissue were analyzed by real time RT-PCR. The results showed that in the treated group with-LIPUS, cartilage defects were totally filled with relatively thin amorphous proliferative tissue and in the control group the defects were left as a dimple in the cartilage defects. Real time RT-PCR analysis showed that cartilage-specific genes expression levels are significantly increased by application of LIPUS after transplantation of ASCs-PCL construct in vivo. The results suggested that low-intensity pulsed ultrasound stimulates ASCs differentiation and induces chondrogenesis at the ASCs-PCL construct in in vivo.

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Ultrasound Effect on Gene Expression of Sex Determining Region Y-box 9 (SOX9) and Transforming Growth Factor β Isoforms in Adipose Stem Cells

Abstract: Background: Cartilage tissue engineering is a promising method for repair of cartilage defects. Induction of chondrogenesis in mesenchymal stem cells (MSC) is currently used in cartilage tissue engineering. Among growth factors, transforming growth factor

Cited by 3 publications

References 23 publications

Conditioned Medium of Wharton's Jelly Derived Stem Cells Can Enhance the Cartilage Specific Genes Expression by Chondrocytes in Monolayer and Mass Culture Systems

Conditioned Medium of Wharton's Jelly Derived Stem Cells Can Enhance the Cartilage Specific Genes Expression by Chondrocytes in Monolayer and Mass Culture Systems

Effects of Low Intensity Ultrasound on the Chondrogenic Differentiation of Adult Stem Cells From Adipose Tissue

Effect of low-intensity pulsed ultrasound on regenerative potential of transplanted ASCs â€“PCL construct in articular cartilage defects in sheep

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