Ultrasound Characteristics of the Cavitary Corpus Luteum after Oestrus Synchronization in Heifers in Relation to the Results of Embryo Transfer

Abstract

The aim of the study was to conduct an ultrasound analysis of quantitative parameters of the corpus luteum (CL) in recipient heifers on days 6–8 after oestrus, and to compare reproduction potential of both types of CL in those females. Analyses were performed on 300 heifers, synchronized with two injections of cloprostenol. Clinical and ultrasound examinations of ovaries were performed and measurements of the CL were recorded. The blood samples were taken to determine progesterone level. Pregnancy examination was conducted after 6–8 weeks from the ET. Cavitary CL was found in 32.7% heifers In 48.0% of the cavitary CL, its luteal tissue area was reduced by 14.3% compared to the compact CL, while 16.3% of the CL had luteal tissue reduced by more than 33.8%. Progesterone level in blood serum was higher in heifers with the cavitary CL (p < 0.001). Pregnancy rate was higher for females with a cavitary CL (52%) than those with compact ones (33%, p < 0.05). The ultrasound assessment of luteal tissue should be included in the evaluation of the functional status of the CL in ET-recipient heifers. The cavitary CL presence may indicate a higher potential of the recipient in maintaining the pregnancy.

Ex Vivo Study of Macroscopic Feature and Sonogram Imaging of Bovine Ovarian Corpus Luteum

“…3F). Ultrasound imaging has been used to identify the structure in the ovary for decades and still develop until now (Pieterse 1989;Boyd and Omran 1991;Viana et al 2013;Jaśkowski et al 2021). In general, CL can be identified using ultrasound 3 days after ovulation.…”

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

“…Between 0 and 7 days after insemination it should amount to 2.71 ng/ml, and between 8-14 days after insemination-1.48 ng/ml. According to Jaśkowski et al [65,66], the type of corpus luteum is also a significant factor correlated with the recorded progesterone levels.…”

Sonografische Verlaufsuntersuchungen zur Bedeutung der Follikelgröße und Gelbkörpermorphologie bei Milchkühen post inseminationem

“…Dies konnte auch bei Embryotransfer-Empfängertieren bestätigt werden [28]. In neueren Studien wurden Empfängertiere mit einem Gelbkörper mit Hohlraum zwischen Tag 6 und 8 sogar häufiger tragend [29,30].…”

“…Insgesamt wiesen 62 % aller Gelbkörper am Tag 9 einen Hohlraum auf. In der Literatur sind Häufigkeiten des Auftretens von Hohlräumen zwischen 25 und 75 % beschrieben, wobei die meisten Studien eher geringere Anteile nachwiesen [9,29,[34][35][36][37][38]. Diese breite Hohlraumhäufigkeitsverteilung in der Literatur könnte aufgrund der verschiedenen Untersuchungstechniken oder durch hormonelle Vorbehandlungen bedingt worden sein.…”