Ultrasound-Assisted Production of Xylo-Oligosaccharides From Alkali-Solubilized Corncob Bran Using Penicillium janthinellum XAF01 Acidic Xylanase

Abstract

A novel treatment involving enzymatic hydrolysis using an acidic xylanase coupled with ultrasound was performed to improve the xylo-oligosaccharides (XOS) yield from corncob bran. The acidic xylanase (XynB) was purified to a most suitable pH, temperature, and operational parameters for ultrasound-assisted hydrolysis were determined. A preliminary mechanistic investigation was performed through circular dichroism (CD) spectroscopy, scanning electron microscope (SEM) and a laser particle size analyzer, and the effects of ultrasound on enzyme (XynB) and substrate (corncob bran) were assessed. The results show that the maximum XOS yield was 20.71% when the reaction pH and temperature were 4.3 and 50°C, the ultrasonic parameters were 50 kHz and 0.40 W/cm2, which was 2.55 fold higher than that obtained using a non-ultrasound-assisted enzymatic preparation. Mechanism studies indicated that ultrasonic pretreatment could reduce the β-fold content and increase the random coil content. Changes in structure and size of substrate were observed. The specific surface area of the XAC molecules is easy to carry out enzymatic reaction, which is beneficial to the production of XOS.

Ultrasonic‐assisted extraction and purification of xylo‐oligosaccharides from wheat bran

“…The extraction rate of xylan increased as ultrasonic frequency increased and reached maximum value (52.07%) at 72 kHz, then tended to decrease. This result is consistent with the study of Zhou et al (2021).…”

Sugar-Based Water Retention Agents in Meat Products: Enhancing Water-Holding Capacity and Promoting Health Benefits

“…Water extraction method (namely solid-liquid extraction method) is widely utilized in the extraction of pectin, FG, inulin and LBG based on the characteristics of strong water penetration to plant tissue, high extraction efficiency, simple operation, safety and economy [288,304,305,328]. As a new extraction process, ultrasound is intended to play a "cavitation effect" to destroy cells, promote the release of plant contents, effectively reduce and eliminate the blocking layer between the water phase, increase the mass transfer efficiency, and contribute to the diffusion of solute [308,310,329]. In the industrial setting, ultrasonic technology is much simpler, takes less time, and relies less on enzymes to extract the required biochemical components of algae [292,330,331].…”