Ultrasound-assisted preparation of protein–polyphenol conjugates and their structural and functional characteristics

Yan, Shizhang; Wang, Qi; Yu, Jiaye; Li, Yang; Qi, Baokun

doi:10.1016/j.ultsonch.2023.106645

Cited by 17 publications

(6 citation statements)

References 40 publications

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“…The location and intensity of the characteristic peaks of the IPA–polyphenol conjugates changed significantly compared with the control IPA. The variation of the characteristic peaks associated with the amide A band suggests that the conjugates involve the reaction of the –NH 2 group of the protein in the covalent interaction 36 . For protein detection, the amide I and amide II bands mainly reflect changes in the secondary structure of the protein.…”

Section: Resultsmentioning

confidence: 99%

“…The variation of the characteristic peaks associated with the amide A band suggests that the conjugates involve the reaction of the -NH 2 group of the protein in the covalent interaction. 36 For protein detection, the amide I and amide II bands mainly reflect changes in the secondary structure of the protein. The characteristic peaks of the amide I and amide II bands in the conjugates were blueshifted or redshifted compared with the control IPA, which was likely due to the strong structural stretching caused by the new formation of the CO-NH (peptide bond) in the conjugates, 37 indicating a change in the secondary structure of IPA in the conjugates.…”

Section: Structural Analysis Of the Ipa-polyphenol Conjugates Ftir An...mentioning

confidence: 99%

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Covalent conjugation of Inca peanut albumin and polyphenols with different phenolic hydroxyl numbers through laccase catalysis to improve functional properties

Wu,

Li,

Liu

et al. 2024

J Sci Food Agric

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BACKGROUNDEnzymatic crosslinking is a method that can be used to modify Inca peanut albumin (IPA) using polyphenols, and provides desirable functionalities; however, the effect of polyphenol structures on conjugate properties is unclear. In this study, we selected four polyphenols with different numbers of phenolic hydroxyl groups [para‐hydroxybenzoic acid (HBA), protocatechuic acid (PCA), gallic acid (GA), and epigallocatechin gallate (EGCG)] for covalent modification of IPA by enzymatic crosslinking, and explored the structure–function changes of the IPA–polyphenol conjugates.RESULTSSodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) analysis showed that laccase successfully promoted covalent crosslinking of IPA with polyphenols, with the order of degree of conjugation as EGCG > GA > PCA > HBA, the IPA–EGCG conjugate showed the highest polyphenol binding equivalents (98.35 g kg−1 protein), and a significant reduction in the content of free amino, sulfhydryl, and tyrosine group. The oxidation of polyphenols by laccase forms quinone or semiquinone radicals that are covalently crosslinked to the reactive groups of IPA, leading to significant changes in the secondary and tertiary structures of IPA, with spherical structures transforming into dense lamellar structures. The 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging ability and emulsification stability of IPA–EGCG conjugates improved by almost 6‐fold and 2.7‐fold, respectively, compared with those of unmodified IPA.CONCLUSIONThese data suggest that the higher the number of polyphenol hydroxyl groups, the higher the degree of IPA–polyphenol conjugation; additionally, enzymatic crosslinking can significantly improve the functional properties of IPA. © 2024 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 99%

Section: Structural Analysis Of the Ipa-polyphenol Conjugates Ftir An...mentioning

confidence: 99%

Covalent conjugation of Inca peanut albumin and polyphenols with different phenolic hydroxyl numbers through laccase catalysis to improve functional properties

Wu,

Li,

Liu

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

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“…UV–Vis spectroscopy can be used to assess changes in protein structure, which include conformational and hydration changes, dissociation, or denaturation [29] . The effects of sonication and L-His on the UV absorption of amino acids by proteins are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasound-assisted modification of soybean protein isolate with L-histidine: Relationship between structure and function

Xu,

Yan,

et al. 2024

Ultrasonics Sonochemistry

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“…Coupled with the inherent antioxidant and anti-inflammatory properties of these polyphenolic compounds, grafting further endows the nanocarriers with additional biological functions (Q. Hu & Luo, 2016;S. Yan, Wang, et al, 2023).…”

Section: Polyphenol-grafted Nanocarriersmentioning

confidence: 99%

“…Additionally, the aromatic ring structure of these compounds offers spatial steric effects, enabling delayed or controlled release of drugs (Kong et al, 2018). Coupled with the inherent antioxidant and anti‐inflammatory properties of these polyphenolic compounds, grafting further endows the nanocarriers with additional biological functions (Q. Hu & Luo, 2016; S. Yan, Wang, et al, 2023). Therefore, using natural polyphenolic compounds derived to optimize nanocarriers' biocompatibility and pharmacological efficacy represents an emerging trend in nanomedicine.…”

Section: Development Of Nanocarriers Based On Plant‐derived Active Co...mentioning

confidence: 99%

The development of nanocarriers for natural products

Huang,

Luo,

Tong

et al. 2024

WIREs Nanomed Nanobiotechnol

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Natural bioactive compounds from plants exhibit substantial pharmacological potency and therapeutic value. However, the development of most plant bioactive compounds is hindered by low solubility and instability. Conventional pharmaceutical forms, such as tablets and capsules, only partially overcome these limitations, restricting their efficacy. With the recent development of nanotechnology, nanocarriers can enhance the bioavailability, stability, and precise intracellular transport of plant bioactive compounds. Researchers are increasingly integrating nanocarrier‐based drug delivery systems (NDDS) into the development of natural plant compounds with significant success. Moreover, natural products benefit from nanotechnological enhancement and contribute to the innovation and optimization of nanocarriers via self‐assembly, grafting modifications, and biomimetic designs. This review aims to elucidate the collaborative and reciprocal advancement achieved by integrating nanocarriers with botanical products, such as bioactive compounds, polysaccharides, proteins, and extracellular vesicles. This review underscores the salient challenges in nanomedicine, encompassing long‐term safety evaluations of nanomedicine formulations, precise targeting mechanisms, biodistribution complexities, and hurdles in clinical translation. Further, this study provides new perspectives to leverage nanotechnology in promoting the development and optimization of natural plant products for nanomedical applications and guiding the progression of NDDS toward enhanced efficiency, precision, and safety.This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

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Ultrasound-assisted preparation of protein–polyphenol conjugates and their structural and functional characteristics

Cited by 17 publications

References 40 publications

Covalent conjugation of Inca peanut albumin and polyphenols with different phenolic hydroxyl numbers through laccase catalysis to improve functional properties

Covalent conjugation of Inca peanut albumin and polyphenols with different phenolic hydroxyl numbers through laccase catalysis to improve functional properties

Ultrasound-assisted modification of soybean protein isolate with L-histidine: Relationship between structure and function

The development of nanocarriers for natural products

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