Ultrasound-assisted Peptide Nucleic Acids synthesis (US-PNAS)

Bene, Alessandra Del; D'Aniello, Antonia; Tomassi, Stefano; Merlino, Francesco; Mazzarella, Vincenzo; Russo, Rosita; Cosconati, Sandro; Maro, Salvatore Di; Messere, Anna

doi:10.1016/j.ultsonch.2023.106360

Cited by 3 publications

(4 citation statements)

References 50 publications

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Section: Resultsmentioning

confidence: 99%

“…Literature reports suggest that PNA chemisorption is a two-step process and a high probe concentration in the immobilization medium is needed to obtain the desired conformation [ 33 ]. Given the reported problems in PNA SAMs formation, as well as their generally high cost and the inconsistency of preparation protocols (some recommend the addition of trifluoroacetic acid and/or organic solvent to improve PNA solubility [ 34 ]), we concluded that they were not competitive with classical DNA probes and did not continue further optimization.…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure 3a, DNA analogues (ZNA ® and PNA) had significantly lower immobilization efficiency than regular DNA. The surface density of ZNA ® and PNA reached 17% and 19% of the density observed for the DNA probe, respectively [33,34]. A plausible explanation for the observed phenomena lies in the complexity of the interaction mechanism of the probes with the gold surface.…”

Section: Purification Of Dna-protein Conjugates and Spr-based Quality...mentioning

confidence: 88%

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A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

Karoń,

Drozd,

Malinowska

2024

Biosensors

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Protein-based microarrays are important tools for high-throughput medical diagnostics, offering versatile platforms for multiplex immunodetection. However, challenges arise in protein microarrays due to the heterogeneous nature of proteins and, thus, differences in their immobilization conditions. This article advocates DNA-directed immobilization (DDI) as a solution, emphasizing its rapid and cost-effective fabrication of biosensing platforms. Thiolated single-stranded DNA and its analogues, such as ZNA® and PNA probes, were used to immobilize model proteins (anti-CRP antibodies and SARS-CoV nucleoprotein). The study explores factors influencing DDI-based immunosensor performance, including the purity of protein-DNA conjugates and the stability of their duplexes with DNA and analogues. It also provides insight into backfilling agent type and probe surface density. The research reveals that single-component monolayers lack protection against protein adsorption, while mixing the probes with long-chain ligands may hinder DNA-protein conjugate anchoring. Conventional DNA probes offer slightly higher surface density, while ZNA® probes exhibit better binding efficiency. Despite no enhanced stability in different ionic strength media, the cost-effectiveness of DNA probes led to their preference. The findings contribute to advancing microarray technology, paving the way for new generations of DDI-based multiplex platforms for rapid and robust diagnostics.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Purification Of Dna-protein Conjugates and Spr-based Quality...mentioning

confidence: 88%

See 1 more Smart Citation

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

Karoń,

Drozd,

Malinowska

2024

Biosensors

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“…62,67,68 72 has significantly enhanced the efficiency of coupling reactions, reduced reaction times, and minimized the production of unwanted by-products, particularly when synthesizing longer peptides. 73 Additionally, the use of automatic synthesizers, microwave methods, 73 continuous flow-assisted 74 and ultrasonicassisted synthesis, 75,76 as stand-alone strategy or in combination, further streamlines the synthesis process. Chemical synthesis offers the rapid and automated production of peptides in large quantities while enabling the incorporation of modified amino acids, linkers, and stabilizers.…”

Section: Adjuvantsmentioning

confidence: 99%

The bright side of chemistry: Exploring synthetic peptide‐based anticancer vaccines

D'Aniello,

Del Bene,

Mottola

et al. 2024

Journal of Peptide Science

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The present review focuses on synthetic peptide‐based vaccine strategies in the context of anticancer intervention, paying attention to critical aspects such as peptide epitope selection, adjuvant integration, and nuanced classification of synthetic peptide cancer vaccines. Within this discussion, we delve into the diverse array of synthetic peptide‐based anticancer vaccines, each derived from tumor‐associated antigens (TAAs), including melanoma antigen recognized by T cells 1 (Melan‐A or MART‐1), mucin 1 (MUC1), human epidermal growth factor receptor 2 (HER‐2), tumor protein 53 (p53), human telomerase reverse transcriptase (hTERT), survivin, folate receptor (FR), cancer‐testis antigen 1 (NY‐ESO‐1), and prostate‐specific antigen (PSA). We also describe the synthetic peptide‐based vaccines developed for cancers triggered by oncovirus, such as human papillomavirus (HPV), and hepatitis C virus (HCV). Additionally, the potential synergy of peptide‐based vaccines with common therapeutics in cancer was considered. The last part of our discussion deals with the realm of the peptide‐based vaccines delivery, highlighting its role in translating the most promising candidates into effective clinical strategies. Although this discussion does not cover all the ongoing peptide vaccine investigations, it aims at offering valuable insights into the chemical modifications and the structural complexities of anticancer peptide‐based vaccines.

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An Efficient Route for the Synthesis of 2-Arylbenzothiazoles in an Ionic Liquid, Using Ultrasound Irradiation

Yu,

Duan,

Wang

et al. 2023

HETEROCYCLES

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Ultrasound-assisted Peptide Nucleic Acids synthesis (US-PNAS)

Cited by 3 publications

References 50 publications

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

The bright side of chemistry: Exploring synthetic peptide‐based anticancer vaccines

An Efficient Route for the Synthesis of 2-Arylbenzothiazoles in an Ionic Liquid, Using Ultrasound Irradiation

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