Ultrasound-assisted Maillard reaction of ovalbumin/xylose: The enhancement of functional properties and its mechanism

Liu, Xuanting; Yang, Qi; Yang, Meng; Du, Zhiyang; Wei, Chen; Zhang, Ting; Zhang, Biying; Liu, Jingbo

doi:10.1016/j.ultsonch.2021.105477

Cited by 61 publications

(25 citation statements)

References 51 publications

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“…We modeled ( Kianmeher et al, 2016 ; Liu et al, 2021 ) two groups of female 6-week-old BALB/C mice: asthma group and blank control group ( n = 3 per group). They had the same feeding conditions and growth environment.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome-Wide m6A Methylome and m6A-Modified Gene Analysis in Asthma

Sun

Shen

et al. 2022

Front. Cell Dev. Biol.

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N6-methyladenosine (m6A) modification is one of the most prevalent RNA modification forms and is an important posttranscriptional mechanism for regulating genes. In previous research, we found that m6A regulator–mediated RNA methylation modification was involved in asthma; however, the specific modified genes are not clear. In this study, we systematically evaluated the transcriptome-wide m6A methylome and m6A-modified genes in asthma. Here, we performed two high-throughput sequencing methods, methylated RNA immunoprecipitation sequencing (MeRIP-seq), and RNA sequencing (RNA-seq) to identify key genes with m6A modification in asthma. Through difference analysis, we found that 416 methylation peaks were significantly upregulated and 152 methylation peaks were significantly downregulated, and it was mainly distributed in 3′ UTR. Furthermore, compared with the control group, there were 2,505 significantly upregulated genes and 4,715 significantly downregulated genes in the asthma group. Next, through a combined analysis of transcriptome and differential peaks, 14 differentially expressed genes related to RNA methylation modification were screened. Finally, through 87 health controls and 411 asthma cases from the U-BIOPRED (Unbiased Biomarkers for the Prediction of Respiratory Disease Outcomes) program, we verified three m6A-modified key genes (BCL11A, MATK, and CD300A) and found that they were mainly distributed in exons and enriched in 3' UTR. Our findings suggested that intervening in m6A-modified genes may provide a new idea for the treatment of asthma.

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Section: Methodsmentioning

confidence: 99%

Transcriptome-Wide m6A Methylome and m6A-Modified Gene Analysis in Asthma

Sun

Shen

et al. 2022

Front. Cell Dev. Biol.

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“…Circular dichroism spectra were measured at 25 • C in the extreme ultraviolet region (200 to 250 nm) using MOS-450 Circular Dichroism Spectrometer (Bio-Logic Science Instruments, Grenoble, France) according to the published method (15). The protein concentration was 0.2 mg/ml, and the path length was 1 mm.…”

Section: Circular Dichroismmentioning

confidence: 99%

“…The emulsifying activity index (EAI) and emulsion stability index (ESI) of OVA (native) and its conjugates were measured according to the turbidimetric method as previously reported (15). A volume of 5 ml of conjugate solution was dissolved in 10 ml of phosphate buffer (0.01 M, pH 7.0) and hydrated for 15 min.…”

Section: Emulsifying Activity and Stability Indicesmentioning

confidence: 99%

“…At present, studies for the emulsifying properties of protein-sugar conjugates mainly focus on the influence of system conditions, such as pH, ionic strength, or heating time. Liu et al found that there were strongly linear correlations between the surface properties of conjugates and DG under different glycation parameters (pH, monosaccharide/protein ratio, heating time) (15). Dong et al demonstrated that the FSG-WPI conjugate prepared by 72 h incubation had the best emulsifying performance in different reaction times and evaluated the antioxidant activity and thermal stability of the conjugates produced (16).…”

Section: Introductionmentioning

confidence: 99%

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Modification of Ovalbumin by Maillard Reaction: Effect of Heating Temperature and Different Monosaccharides

et al. 2022

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Glycosylation is considered to be an effective way to improve the performance of protein emulsification. This study focused on the effects of the molecular structure and emulsifying properties of ovalbumin (OVA) by wet heating Maillard reaction with three types of monosaccharides (i.e., xylose, glucose, and galactose). Results showed that increasing reaction temperature from 55°C to 95°C could significantly improve the degree of grafting (DG), while glycosylated OVA conjugate with xylose at 95°C processed the highest DG of 28.46%. This reaction was further confirmed by the browning intensity determination. Analysis of Fourier transform infrared spectrophotometer, circular dichroism, and fluorescence spectra indicated that there were slight changes in the subunits and the conversion of α-helices to β-sheets, as well as the unfolded structures, thereby increasing the surface hydrophobicity and absolute zeta potential of obtained glycosylated OVA. Glycosylation endowed OVA with better emulsifying properties, especially the xylose glycosylated OVA was superior to that of glucose and galactose glycosylated OVA, which was mainly due to its shorter molecular chains with smaller steric hindrance for reaction. Furthermore, the enhancement of emulsifying properties may be attributed to the synergistic effect of stronger electrostatic repulsion of larger absolute zeta potential and the steric hindrance from thicker adsorbed layer, thereby inhibiting aggregation and flocculation of emulsion droplet.

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“…Meanwhile, extra hydroxyl radicals (OH·) was produced by cavitation bubbles through cleaving additional solvent molecules [19] , giving the opportunity for alkaline reaction. We tentatively hypothesized that the combination of ultrasound and alkaline reaction could act in a synergistic way, whereby the actual effect was generally more superior than only single alkaline oxidation process [20] , [21] , [22] .…”

Section: Introductionmentioning

confidence: 99%