Ultrasound-assisted extraction optimization and validation of ultra-performance liquid chromatographic method for the quantification of miquelianin in Cuphea glutinosa leaves

Santos, Marí Castro; Koetz, Mariana; Mendez, Andreas Sebastian Loureiro; Henriques, Amélia Teresinha

doi:10.1016/j.talanta.2020.120988

Cited by 14 publications

(8 citation statements)

References 14 publications

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“…The extracts from the leaves of C. lindmaniana and C. urbaniana were prepared according to Santos et al [2,3] . performed by ultrasound‐assisted method, where the vegetal material was submitted to an ultrasonic bath (40 kHz, 132 W), [15] under the following conditions: particle size at≤180 μm; plant:solvent ratio 1: 20 (w/v); 40 % ethanol and time 30 min. After extraction, for ACE‐inhibition assay the samples were filtered and lyophilized.…”

Section: Methodsmentioning

confidence: 99%

Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

Santos

Soares

Beltrame

et al. 2021

Chemistry & Biodiversity

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The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves. The extraction was performed ultrasound-assisted, and UHPLC/MS analysis was in positive mode ionization. The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001 -10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils. The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition. From the nineteen proposed compounds, six of them are described for the first time in this genus. The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations. The ACE-inhibition presented results ranging from 19.58 to 22.82 %. In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential. Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.

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Section: Methodsmentioning

confidence: 99%

Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

Santos

Soares

Beltrame

et al. 2021

Chemistry & Biodiversity

Self Cite

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“…To validate the simultaneous analysis, we referred to the relevant ICH guideline [28]. The validation parameters were specificity, linearity, precision, accuracy, and LOD/LOQ.…”

Section: Validation Of Simultaneous Quercetin-3-o-glucuronide and Ellagic Acid Content Analysis Methodsmentioning

confidence: 99%

“…Consequently, the LOD/LOQ values were found to be 0.5 and 1.5 µg/mL, respectively, for quercetin-3-O-glucuronide and 1.0 and 3.0 µg/mL, respectively, for ellagic acid (Table 1). In a recent study on the development of a validated method for quercetin-3-O-glucuronide quantification in Cuphea glutinosa, an R 2 value of 0.9999 was reported, and LOD/LOQ values were 8.41/25.51 µg/mL [28]. Thus, the LOD/LOQ values for this method were approximately 17 fold higher than those of our simultaneous determination method, suggesting that our method can be used more effectively to detect and quantify small amounts of quercetin-3-O-glucuronide.…”

Section: Limit Of Determination and Limit Of Quantificationmentioning

confidence: 99%

Development and Validation of a Method for Determining the Quercetin-3-O-glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD

Kim

Park²,

Kim

et al. 2021

Molecules

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Toward the standardization of common evening primrose (Oenothera biennis) sprout extract (OBS-E), we aimed to obtain indicator compounds and use a validated method. HPLC-UVD allowed simultaneous quantification of the indicator compounds quercetin-3-O-glucuronide and ellagic acid. The method was validated in terms of specificity, linearity, precision, accuracy, and limit of detection/limit of quantification (LOD/LOQ). High specificity and linearity was demonstrated, with correlation coefficients of 1.0000 for quercetin-3-O-glucuronide and 0.9998 for ellagic acid. The LOD/LOQ values were 0.486/1.472 μg/mL for quercetin-3-O-glucuronide and 1.003/3.039 μg/mL for ellagic acid. Intra-day and inter-day variability tests produced relative standard deviation for each compound of <2%, a generally accepted precision criterion. High recovery rate were also obtained, indicating accuracy validation. The OBS-E prepared using various concentrations of ethanol were then analyzed. The 50% ethanol extract had highest content of quercetin-3-O-glucuronide, whereas the 70% ethanol extract possessed the lowest. However, the ellagic acid content was highest in the 70% ethanol extract and lowest in the 90% ethanol extract. Thus, quercetin-3-O-glucuronide and ellagic acid can be used industrially as indicator compounds for O. biennis sprout products, and our validated method can be used to establish indicator compounds for other natural products.

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“…Consequently, the LOD/LOQ values were found to be 0.486 and 1.472 μg/mL, respectively, for quercetin 3-glucuronide and 1.003 and 3.039 μg/mL, respectively, for ellagic acid (Table 1). In a recent study on the development of a validated method for quercetin 3-glucuronide quantification in Cuphea glutinosa, an R 2 value of 0.9999 was reported, and LOD/LOQ values were 8.41/25.51 μg/mL [29]. Thus, the LOD/LOQ values for this method were approximately 17 fold higher than those of our simultaneous determination method, suggesting that our method can be used more effectively to detect and quantify small amounts of quercetin 3-glucuronide.…”

Section: Limit Of Determination and Limit Of Quantificationmentioning

confidence: 99%

Development and Validation of Method for Determining Quercetin 3-Glucuronide and Ellagic Acid Content of Common Evening Primrose (<em>Oenothera biennis</em>) by HPLC-UVD

Kim¹,

Park²,

Hoon³

et al. 2020

Preprint

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Toward the standardization of O. biennis sprout extract (OBS-E), we aimed to obtain indicator compounds, using a validated method. HPLC-UVD allowed simultaneous quantification of indicator compounds quercetin 3-glucuronide and ellagic acid. The method was validated in terms of specificity, linearity, precision, accuracy, and limit of detection/limit of quantification (LOD/LOQ). High specificity and linearity was demonstrated, with correlation coefficients of 1.0000 for quercetin 3-glucuronide and 0.9998 for ellagic acid. The LOD/LOQ values were 0.486/1.472 μg/mL for quercetin 3-glucuronide and 1.003/3.039 μg/mL for ellagic acid. Intra-day and inter-day variability tests produced relative standard deviation for each compound of &lt;2%, a generally accepted precision criterion. High recovery rate were also obtained, indicating accuracy validation. The OBS-E prepared using various concentrations of ethanol were then analyzed. The 50% ethanol extract had highest content of quercetin 3-glucuronide, whereas the 70% ethanol extract possessed the lowest. However, the ellagic acid content was highest in the 70% ethanol extract and lowest in the 90% ethanol extract. Thus, quercetin 3-glucuronide and ellagic acid can be used industrially as indicator compounds for O. biennis sprout products, and our validated method can be used to establish indicator compounds for other natural products.

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Ultrasound-assisted extraction optimization and validation of ultra-performance liquid chromatographic method for the quantification of miquelianin in Cuphea glutinosa leaves

Cited by 14 publications

References 14 publications

Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

Polyphenolic Composition and in Vitro Antihypertensive and Anti‐Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana

Development and Validation of a Method for Determining the Quercetin-3-O-glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD

Development and Validation of Method for Determining Quercetin 3-Glucuronide and Ellagic Acid Content of Common Evening Primrose (<em>Oenothera biennis</em>) by HPLC-UVD

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