Ultrasound-assisted extraction of phycobiliproteins from Spirulina (Arthrospira) platensis using protic ionic liquids as solvent

Rodrigues, Renata Débora Pinto; Castro, Felipe Carolino de; Santiago-Aguiar, Rílvia Saraiva de; Rocha, Maria Valderez Ponte

doi:10.1016/j.algal.2018.02.021

Cited by 71 publications

(36 citation statements)

References 46 publications

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“…As well as its application in the biotechnological and medicine areas, PE can be used in the food and cosmetic industries [8,9]. Commercial PE from microalgae has been produced by Spirulina , Rhodella , and Porphyridium [10,11,12]. The market price of this purified pigment is around $200 USD mg −1 (Sigma-Aldrich, product number: 52412-1MG-F), this being why PE is regarded as a molecule of high value [13].…”

Section: Introductionmentioning

confidence: 99%

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

et al. 2019

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Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.

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Section: Introductionmentioning

confidence: 99%

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

et al. 2019

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“…Phycocyanobilin, phycourobilin, and phycoerythrobilin are phycobilins. The tetrapyrrolic structure of these chromophores is involved in light harvesting of cyanobacteria such as Arthrospira (Akimoto et al 2012;Rodrigues et al 2018). Great binding energies were obtained for these compounds from Autodock Vina and SwissDock with respectively − 7.45 and − 10.35 kcal.mol −1 , − 7.25 and − 9.355 kcal.mol −1 , and − 7.1 and − 9.285 kcal.mol −1 for phycoerythrobilin, phycocyanobilin, and phycourobilin.…”

Section: Discussionmentioning

confidence: 99%

Docking and in silico toxicity assessment of Arthrospira compounds as potential antiviral agents against SARS-CoV-2

Petit¹,

Vernès²,

Cadoret³

2021

J Appl Phycol

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A race is currently being launched as a result of the international health situation. This race aims to find, by various means, weapons to counter the Covid-19 pandemic now widespread on all continents. The aquatic world and in particular that of photosynthetic organisms is regularly highlighted but paradoxically little exploited in view of the tremendous possibilities it offers. Computational tools allow not only to clear the existence and activity of many molecules but also to model their relationships with receptors identified in potential hosts. On a routine basis, our laboratory carries out a research activity on functionalities of molecules derived from algae using in silico tools. We have implemented our skills in algae biology and in modeling, as tests in order to identify molecules expressed by the genus Arthrospira showing an antiviral potential and more particularly anti-SARS-CoV-2. Using consensus docking and redocking with Autodock Vina and SwissDock, we were able to identify several promising molecules from Arthrospira: phycocyanobilin, phycoerythrobilin, phycourobilin, and folic acid. These four compounds showed reliable binding energies comprised between − 6.95 and − 7.45 kcal.mol −1 in Autodock Vina and between − 9.285 and − 10.35 kcal.mol −1 with SwissDock. Toxicity prediction as well as current regulations provided promising arguments for the inclusion of these compounds in further studies to assess their ability to compete with the SARS-CoV-2/ACE2 complex both in vitro and in vivo.

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“…The tetrapyrrolic structure of these chromophores is involved in light harvesting of cyanobacteria such as Arthrospira (Akimoto et al 2012;Rodrigues et al 2018). Phycocyanin and phycoerythrin are authorized by both FDA and European Commission and are usually used as food coloring (Bratinova 2015).…”

Section: Discussionmentioning

confidence: 99%

Docking and in silico toxicity assessment of Arthrospira compounds as potential antiviral agents against SARS-CoV-2

Petit¹,

VERNES²,

Cadoret³

2020

Preprint

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Abstract A course is currently being launched as a result of the international health situation. This race aims to find, by various means, weapons to counter the Covid-19 pandemic now widespread on all continents. The aquatic world and in particular that of photosynthetic organisms is regularly highlighted but paradoxically little exploited in view of the tremendous possibilities it offers. Computational tools allow not only to clear the existence and activity of many molecules, but also to model their relationships with receptors identified in potential hosts. On a routine basis, our laboratory carries out a research activity on functionalities of molecules derived from algae, using in silico tools. We have implemented our skills, in algae biology and in modeling, as tests in order to identify molecules expressed by the genus Arthrospira showing an antiviral potential and more particularly anti SARS-CoV-2. Using Autodock Vina, we were able to identify 3 molecules: phycocyanobilin, phycoerythrobilin and folic acid. These 3 compounds showed binding energies able to compete with the SARS-CoV-2/ACE2 complex. Toxicity prediction as well as current regulations support their use as potential candidates for the fight against Covid-19.

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Ultrasound-assisted extraction of phycobiliproteins from Spirulina (Arthrospira) platensis using protic ionic liquids as solvent

Cited by 71 publications

References 46 publications

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Docking and in silico toxicity assessment of Arthrospira compounds as potential antiviral agents against SARS-CoV-2

Docking and in silico toxicity assessment of Arthrospira compounds as potential antiviral agents against SARS-CoV-2

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