Ultrasound-assisted enzymatic hydrolysis of cassava waste to obtain fermentable sugars

Leaes, Eloisa X.; Zimmermann, E.; Souza, Matheus de Freitas; Ramon, Adriana P.; Mezadri, Ethiane T.; Prá, Valéria Dal; Terra, Lisiane M.; Mazutti, Márcio A.

doi:10.1016/j.biosystemseng.2013.02.001

Cited by 24 publications

(16 citation statements)

References 16 publications

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“…The desirability function allows the response surface produced be inspected by fitting the observed responses using the above mentioned equation based on levels of the independent variables. This equation was used to predict values for response (inulinase immobilization yield) at different combinations of levels of the independent variables, specify desirability functions for the dependent variables, and to search for the levels of the independent variables that produce the most desirable responses for the dependent variables (immobilization yield) [13].…”

Section: Central Composite Rotational Design (2 2 )mentioning

confidence: 99%

Immobilization of commercial inulinase on alginate–chitosan beads

Missau

Scheid

Foletto

et al. 2014

Sustain Chem Process

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The commercial inulinase obtained from Aspergillus niger was effectively immobilized on alginate-chitosan beads which were hardened with glutaraldehyde. The immobilization conditions were studied using Plackett & Burmann experimental design and central composite rotational design (CCRD). The effects of chitosan, glutaraldehyde, sodium alginate and calcium chloride concentrations in order to obtain a better immobilization yield were optimized. In the Plackett & Burman experimental design, the sodium alginate and calcium chloride had a significant effect (p < 0.1), but only the calcium chloride showed a positive effect, indicating that as higher the concentration, better is the immobilization yield. In the central composite rotational design (CCRD), the best results were obtained in the central point, using sodium alginate (1% w/v) and calcium chloride (4% w/v) as conditions for inulinase immobilization. By the CCRD, the optimal immobilization strategy was: chitosan (0.1% w/v), glutaraldehyde (0.1% v/v), sodium alginate (1% w/v) and calcium chloride (4% w/v). In this condition, the enzyme loading capacity was 668 U/g gel beads and the effect of temperature on the immobilized enzyme activity was also evaluated, showing better activity at 50°C. The immobilized enzyme maintained 76% of its activity in six days at room temperature.

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Section: Central Composite Rotational Design (2 2 )mentioning

confidence: 99%

Immobilization of commercial inulinase on alginate–chitosan beads

Missau

Scheid

Foletto

et al. 2014

Sustain Chem Process

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“…These residues are used for animal feed, but causes serious environmental problems. Cassava residue is rich in fiber and residual starch (Leaes et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…In this way, enzymatic hydrolysis has been used as a technique that allows the transformation of unfermentescivel starch into fermentable sugars, which in turn can be converted into various products of interest, such as ethyl alcohol (Serrano & Franco, 2005;Rattanachomsri et al, 2009;Leaes et al, 2013;Sugumaran et al, 2014). There are few reports on the reuse of cassava peel, which is mainly destined for direct animal feeding, therefore, the aim of this study was to characterize the residue from cassava pre-cleaning and evaluate its enzymatic hydrolysis, enabling its use in fermentation processes for the production of various products such as alcohol and biofuels.…”

Section: Introductionmentioning

confidence: 99%

Utilization of residue from cassava starch processing for production of fermentable sugar by enzymatic hydrolysis

et al. 2016

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The aim of this study was to characterize and perform enzymatic hydrolysis of cassava peeling residue (peel and inner peel), mainly composed of peels and small pieces. Residue was sanitized, dried at 55 °C for 24 hours and ground. The obtained flour showed pH of 4.85; 72.53 g 100 g -1 moisture; 5.18 mL 1M NaDH 100 g -1 acidity; 60.68 g 100 g -1 starch; 1.08 g 100 g -1 reducing sugar; 1.63 g 100g -1 ash; 0.86 g 100 g -1 lipid and 3.97 g 100 g -1 protein. Enzymatic hydrolysis was carried out by means of rotational central composite design, analyzing the effects of concentrations of α-amylase enzyme (10 to 50 U g starch ) on variable responses: percent conversion of starch into reducing sugars (RSC) and soluble solid content (SS). Highest values of RSC (110%) and SS (12 °Brix) were observed when using the maximum concentration of amyloglucosidase and throughout the concentration range of α-amylase. Enzymatic hydrolysis of cassava peel is feasible and allows the use of hydrolysate in fermentation processes for the production of various products, such as alcoholic drinks, vinegar, among others.

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“…However, it is expected that this increase in the activity occurs only for short period [11]. In previous works, we have also verified significant modifications in the effects of process variables, namely temperature, pH, solid to liquid ratio and enzyme load using ultrasound [12,20,21].…”

Section: Resultsmentioning

confidence: 69%

Enzymatic hydrolysis of non-treated sugarcane bagasse using pressurized liquefied petroleum gas with and without ultrasound assistance

et al. 2015

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a b s t r a c tIn this work the enzymatic hydrolysis of sugarcane bagasse using pressurized liquefied petroleum gas (LPG) and ultrasound assisted pressurized LPG (US assisted-LPG) to obtain fermentable sugar in a unique step was investigated. For this purpose, non-treated sugarcane bagasse was used for enzymatic hydrolysis in a fixed-bed bioreactor. Several experiments were carried out to investigate the influence of moisture content, enzyme load and pressure on the yield of fermentable sugar after the hydrolysis. Maximum yield of fermentable sugar for hydrolysis carried out using pressurized LPG and US assisted-LPG was 245 and 217 g kg À1 , respectively. The main contribution of this work was to demonstrate that the use of alternative technologies (US assisted-LPG) has the potential to increase the efficiency of enzymatic hydrolysis of lignocellulosic materials without the needs of hard pre-treatment of the material and high enzyme loading.

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Ultrasound-assisted enzymatic hydrolysis of cassava waste to obtain fermentable sugars

Cited by 24 publications

References 16 publications

Immobilization of commercial inulinase on alginate–chitosan beads

Immobilization of commercial inulinase on alginate–chitosan beads

Utilization of residue from cassava starch processing for production of fermentable sugar by enzymatic hydrolysis

Enzymatic hydrolysis of non-treated sugarcane bagasse using pressurized liquefied petroleum gas with and without ultrasound assistance

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