Ultrasound assisted aqueous two-phase extraction of polysaccharides from corn stigma: Process optimization, structure characterization, and immunomodulatory activity

Xue, Hongkun; Wang, Wenli; Wu, Jiping; Xie, Kaifang; Ge, Shaoqin; Tan, Jiaqi

doi:10.1016/j.ifset.2023.103531

Innovative Food Science & Emerging Technologies

2024

DOI: 10.1016/j.ifset.2023.103531

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Ultrasound assisted aqueous two-phase extraction of polysaccharides from corn stigma: Process optimization, structure characterization, and immunomodulatory activity

Hongkun Xue,

Wenli Wang,

Jiping Wu

et al.

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2024

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Cited by 3 publications

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Structural characteristics and anti-tumor activity of alcohol-precipitated polysaccharides from Cordyceps militaris under different ethanol concentrations

Dai,

Wang,

et al. 2024

International Journal of Biological Macromolecules

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Structural characteristics and anti-tumor activity of alcohol-precipitated polysaccharides from Cordyceps militaris under different ethanol concentrations

Dai,

Wang,

et al. 2024

International Journal of Biological Macromolecules

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Solvent bar micro-extraction (SBME) of analytes from complex matrices: Transition towards green solvents

Musarurwa

2024

Microchemical Journal

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Ultrasound assisted hot water extraction of polysaccharides from Taraxacum mongolicum: Optimization, purification, structure characterization, and antioxidant activity

Xue,

Nima,

Wang

et al. 2024

Journal of Food Science

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Ultrasound assisted hot water extraction (UAHWE) was applied to extraction of polysaccharides from Taraxacum mongolicum with hot water as extract solvent. Experimental factors in UAHWE process were optimized by response surface methodology. The optimal extraction parameters to achieve the highest Taraxacum mongolicum polysaccharides (TMPs) yield (12.08 ± 0.14)% by UAHWE were obtained under the ultrasound power of 200 W, extraction temperature of 62°C, solid‐to‐liquid ratio of 1:20 g/mL, and extraction time of 40 min, and then the crude TMPs were further purified by DEAE‐52 and Sephadex G‐100 chromatography to obtain a homogenous polysaccharide fraction (TMPs‐1‐SG). Subsequently, the structure of TMPs‐1‐SG was characterized by UV‐vis, Fourier transform infrared spectroscopy (FT–IR), high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), scanning electron microscope (SEM), transmission electron microscopy (TEM), and Congo red test. The results display that TMPs‐1‐SG with an average molecular weight of 5.49 × 104 Da was comprised of mannose (Man), galactose (Gal), xylose (Xyl), and arabinose (Ara) with a molar ratio of 39.85:52.61:27.14:6.30. Moreover, TMPs‐1‐SG did not contain a triple helix structure. Furthermore, TMPs‐1‐SG and TEM presented a sheet‐like, rod‐shaped, and irregular structure. Finally, the antioxidant activity of TMPs‐1‐SG was evaluated by in vitro experiment. The IC50 values of scavenging DPPH and OH radicals for TMPs‐1‐SG achieved 0.71 mg/mL and 0.75 mg/mL, respectively. The findings can provide an effective method for extracting polysaccharides from natural resources.

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Ultrasound assisted aqueous two-phase extraction of polysaccharides from corn stigma: Process optimization, structure characterization, and immunomodulatory activity

Cited by 3 publications

References 44 publications

Structural characteristics and anti-tumor activity of alcohol-precipitated polysaccharides from Cordyceps militaris under different ethanol concentrations

Structural characteristics and anti-tumor activity of alcohol-precipitated polysaccharides from Cordyceps militaris under different ethanol concentrations

Solvent bar micro-extraction (SBME) of analytes from complex matrices: Transition towards green solvents

Ultrasound assisted hot water extraction of polysaccharides from Taraxacum mongolicum: Optimization, purification, structure characterization, and antioxidant activity

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