Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responses

Dobránszki, Judit; Asbóth, Georgina; Homoki, Dávid; Bíró-Molnár, Piroska; Silva, Jaime A. Teixeira da; Remenyik, Judit

doi:10.1016/j.plaphy.2017.10.022

Cited by 22 publications

(47 citation statements)

References 35 publications

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“…Significantly regulated DEGs for annexins, ZFPs, chitinases, and several stress-related enzymes such as methionine sulfoxide reductase and GST, which scavenge reactive oxygen species (ROS), were detected. The primary role of GST in scavenging ROS was already reported in an earlier paper related to activation of the antioxidant system of in vitro potato plantlets after ultrasonication (Dobránszki et al 2017). Thus, upregulation of six DEGs encoding GST confirms our earlier finding, similar to the up-regulation of other ROSscavenging enzymes, like catalase.…”

Section: Stress-related Enzymesmentioning

confidence: 61%

“…All plant material and in vitro culture conditions were identical to those employed by Dobránszki et al (2017Dobránszki et al ( , 2019 and Teixeira da . In brief, single-node stem segments of "Desirée," including a single leaf, were excised from 4-weekold in vitro plantlets and plated for 4 weeks on Murashige and Skoog (1962) medium free of plant growth regulators (PGRs).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Our group has used in vitro plants of the model plant, potato (Solanum tuberosum L.), to advance our understanding of how US impacts plant growth in vitro. An initial biochemical study revealed that the activity of three antioxidant enzymes, glutathione reductase (GR), superoxide dismutase (SOD), and ascorbate peroxidase (APX), increased significantly after 24 h in response to US (20 min of 35 kHz, at 70 W) (Dobránszki et al 2017). Curiously, US-treated plantlets showed 20% longer shoots than control plantlets (not exposed to US) and 24% higher shoot fresh weight (Dobránszki et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…An initial biochemical study revealed that the activity of three antioxidant enzymes, glutathione reductase (GR), superoxide dismutase (SOD), and ascorbate peroxidase (APX), increased significantly after 24 h in response to US (20 min of 35 kHz, at 70 W) (Dobránszki et al 2017). Curiously, US-treated plantlets showed 20% longer shoots than control plantlets (not exposed to US) and 24% higher shoot fresh weight (Dobránszki et al 2017). In a bid to assess what changes might be occurring at the transcriptome level, additional studies were conducted.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Response of In Vitro Potato (Solanum tuberosum L.) to Piezoelectric Ultrasound

et al. 2020

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As part of a wider project to assess the impact of ultrasound on in vitro plant growth, this paper aimed to determine whether the application of piezoelectric ultrasound (PE-US) would induce changes to the transcriptome of in vitro potato (Solanum tuberosum L.). After exposing explants (single-node segments with a single leaf) to PE-US (35 kHz; 70 W) for 20 min, the effect of this stressor was determined at 0 h, 24 h, 48 h, 1 week, and 4 weeks to assess the possible immediate and residual effects of PE-US on the potato transcriptome. After mRNA was isolated, bioinformatic processing and analysis of RNA-seq datasets, KEGG analysis revealed that 24% of up-regulated biological processes were a response to stress immediately after the application of PE-US (reducing to 11% and 9% at 48 h and 4 weeks, respectively). After assessing a total of 40,430 genes for expression intensity at these five time intervals, it was found that 138, 72, 18, 5, and 59 differentially expressed genes (DEGs), respectively, were significantly up-regulated, while 6, 82, 96, 172, and 107 DEGs, respectively, were down-regulated. DEGs coding for universal stress protein, chitinase, catalase, zinc finger proteins, 21 transcription factors, glutathione S-transferase, and 17 heat shock proteins, mainly Hsp70, Hsp20, and Hsp90 proteins, and possibly the first of such discovery in US-based plant stress research, was detected. Following the validation of RNA-seq data by RT-qPCR, between SeqMonk LFC and RT-qPCR LFC, the Spearman and Pearson correlation coefficients were 0.86 and 0.90, respectively. Plantlets exposed to PE-US had significantly shorter shoots but significantly longer roots as well as higher shoot and root fresh weight, while chl b and total chl were significantly lower but the chl a/b ratio was significantly higher in plantlets exposed to PE-US. PE-US thus constituted an acute abiotic stress, but by 4 weeks, in vitro potato plantlets managed to mitigate the stress through the production of antioxidant enzyme systems and other functional metabolic changes.Key Message Piezoelectric ultrasound (PE-US) constitutes a nonfatal abiotic stress to in vitro potato. A notable discovery was the expression of 17 heat shock proteins in response to this abiotic stress.Electronic supplementary material The online version of this article (https://doi.

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Section: Stress-related Enzymesmentioning

confidence: 61%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Response of In Vitro Potato (Solanum tuberosum L.) to Piezoelectric Ultrasound

et al. 2020

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“…Following Dobránszki et al (2017), single-node stem segments with a single leaf cut from 4-week (w)-old in vitro potato plantlets (cv. Desirée) served as the cut explants for this experiment.…”

Section: Plant Materials and Tissue Culture Protocolmentioning

confidence: 99%

mRNA transcription profile of potato (Solanum tuberosum L.) in response to explant cutting

Silva

Hidvégi

Gulyás

et al. 2019

Plant Cell Tiss Organ Cult

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The explant is an important parameter in plant tissue culture. Most plant tissue culture studies in the literature have focused almost exclusively on the use of explants as a tool for organogenesis or (micro)propagation, and there is limited information that documents the changes that occur in explants in response to cutting during explant preparation just prior to or during culture. To better understand the molecular changes occurring during the wounding process that accompanies the preparation of an explant, this study examined the mRNA transcription profile of 4-week-old potato (Solanum tuberosum L. cv. Desirée) single-node stem segments. To achieve this, RNA-seq analysis was performed to identify significantly differentially expressed genes (DEGs) that existed between uncut in vitro plantlets and cut explants. DEGs corresponding to specific enzyme profiles were broadly connected to cellular processes in the extracellular region, nucleus, and plasma membrane, and were associated with biosynthesis, carbohydrate metabolism and catabolism, cellular protein modification, growth and development, and response to stress. The RNA-seq data of eight highly up-or down-regulated DEGs was validated by RT-qPCR, showing high positive Spearman and Pearson correlation coefficients (0.73 and 0.95, respectively) between SeqMonk LFC and RT-qPCR LFC. These results provide a novel perspective of the changes taking place within explants when they are cut and serve as a valuable basis for the study of explant-related stress in plant tissue culture in other species. In addition, our findings may contribute to an understanding of the success or failure of the tissue culture protocol and subsequent establishment of in vitro cultures by better appreciating the stress-related molecular changes taking place. Key message mRNA transcription profiles were used to show how differentially expressed genes in cut stem explants of potato during preparation for plant tissue culture constitutes an abiotic stress, although not fatal.

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Transcription Profile of Potato (Solanum tuberosum L.) Growing In Vitro

Dobránszki

Hidvégi

Gulyás

et al. 2020

J Plant Growth Regul

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Despite countless papers on plant tissue culture, few have assessed the transcriptional changes that occur in a developing explant from the moment it is cut and plated, and during early stages of development. In this study, the mRNA profile of in vitro potato (Solanum tuberosum L.) plantlets derived from internode stems was assessed via four comparisons during five time intervals (0 h, 24 h, 48 h, 1 week, and 4 weeks) using the Kyoto Encyclopedia of Genes and Genomes (KEGG) classification. After screening KEGG metabolic groups, 35 processes were shown to be either up- or down-regulated. The vast majority (28 processes) were related to growth or development. The expression intensity of 40,430 genes was assessed and 158, 107, 163, and 142 sequences were either significantly up- or down-regulated at 0 h vs 24 h, 24 h vs 48 h, 48 h vs 1 week, and 1 week vs 4 weeks, respectively. A total of 10 DEGs coding for transcription factors were significantly down-regulated, including five ethylene-responsive transcription factors, and two probable WRKY transcription factors. RT-qPCR was used to validate RNA-seq data: Spearman and Pearson correlation coefficients between SeqMonk LFC and RT-qPCR LFC were 0.748 and 0.879, respectively. To the best of our knowledge, this is the first transcriptomic assessment of the changes taking place in the mRNA profile of a developing potato stem explant.

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Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responses

Cited by 22 publications

References 35 publications

Transcriptomic Response of In Vitro Potato (Solanum tuberosum L.) to Piezoelectric Ultrasound

Transcriptomic Response of In Vitro Potato (Solanum tuberosum L.) to Piezoelectric Ultrasound

mRNA transcription profile of potato (Solanum tuberosum L.) in response to explant cutting

Transcription Profile of Potato (Solanum tuberosum L.) Growing In Vitro

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