Ultrasonication of chitosan and chitosan nanoparticles

Tang, Elaine S. K.; Huang, Min; Lim, Lee Yong

doi:10.1016/s0378-5173(03)00408-3

Cited by 197 publications

(98 citation statements)

References 17 publications

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“…It is already well documented that the increase in time or amplitude of sonication makes the particles smaller [16,17]. However, our results show that further increases in either of the variables may lead to an increase in the particle size.…”

Section: Resultssupporting

confidence: 38%

Chitosan Nanoparticles for siRNA Delivery: Optimization of Processing/Formulation Parameters

Gharehdaghi

Amani²,

Khoshayand³

et al. 2014

Nucleic Acid Therapeutics

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Chitosan nanoparticles were prepared using ultrasonication methodology at specific amplitudes and times of sonication. Subsequently, small interfering RNA (siRNA) was added to the solution at predetermined values of nitrogen to phosphorous ratio (N/P), and stirring time. Employing response surfaces generated from a statistical model, the effect of sonication time and amplitude, stirring time, and N/P ratio was studied on the particle size, polydispersity, and loading efficiency of prepared siRNA/chitosan nanoparticles. It was found that to obtain the smallest size, amplitude and time of sonication as well as stirring time should be kept at *45%, 165 seconds, and 50 minutes, respectively. Minimum polydispersity values were also obtained at similar values of sonication time/ amplitude and stirring time in addition to N/P values of *28. Also, the maximum proportion of siRNA loading was observed at approximate values of 300 seconds, 80% and 280 for sonication time, amplitude, and N/P ratio, respectively. The optimum conditions (i.e., to prepare a sample with minimum values of particle size and polydispersity index and maximum values of loading efficiency) were determined as 60.6, 30.0 (seconds), 28.0, and 12.5 (minutes) for amplitude, time of sonication, N/P, and stirring time, respectively. In this scrutiny, the predicted values of optimum formulation were 456 nm size, 89.6% loading efficiency, and 0.4 polydispersity index.

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Section: Resultssupporting

confidence: 38%

Chitosan Nanoparticles for siRNA Delivery: Optimization of Processing/Formulation Parameters

Gharehdaghi

Amani²,

Khoshayand³

et al. 2014

Nucleic Acid Therapeutics

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“…By increasing the mesh size, the average size distribution and PDI both increased expectedly. affecting the degree of deacetylation of chitosan samples [27].…”

Section: Resultsmentioning

confidence: 99%

Highly Monodisperse Chitosan Nanoparticles Prepared by a Combined Triple-Method for Potential Use as Drug Carriers

Sadeghi¹

2017

Int J Nanomater Nanotechnol Nanomed

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Chitosan (CS) as a biodegradable polymer with unique bio-attachment properties that makes it favorable to be used in biomedical applications. Insolubility in water is the problem with use of CS. The purpose of this study was to prepare low molecular weight, water-soluble CS nanoparticles that exhibit excellent water solubility and biological, chemical, and physical functions. Oxidative degradation technique using hydrogen peroxide (H 2 O 2 ) was used to decrease chitosan molecular weight. Then ultrasonication and ionic gelation method using sodium tripolyphosphate (TPP) were used to prepare CS nanoparticles. Molecular weight of chitosan determined by Ubbelohde viscometry and it decreased by approximately 100%. From the spectral information (FTIR) it was observed that the cross-linking between CS and TPP was taking place while the main structure of chitosan was the same. The dynamic light scattering results showed that water-soluble nanoparticles had a multimodal size distribution pattern, while low molecular particles yielded monodisperse particle distribution with an average size of ~45 nm, which was directly ascribed to the role of ultrasonication process. The morphology of nanoparticles was observed by SEM and TEM techniques. The mean diameter of nanoparticles was obtained in a range of 30 nm to 45 nm with a narrow size distribution and polydispersity index smaller than 0.2. Cytotoxicity of CS nanoparticles on human umbilical valve endothelial cells (HUVEC) was assessed by CCK-8 assay, as well as "Live/ Dead" assay and subsequent fl uorescent imaging. The results showed no to minimal cytotoxicity for CS solutions up to 50 μg/ml, while sporadic dead cells observed for 100 μg/ml solutions. This suggested that our monodisperse nanoparticle systems are great candidates to be used for drug delivery systems.

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“…The use of TPP-chitosan in latent fingerprint enhancement was significantly affected by TPP : chitosan ratio, it was also expected that the storage temperature [4], concentration [18], molar mass [4,[15][16][17][18], and levels of aggregation, charge, and degree of deacetylation (DD) of chitosan will be of importance. Furthermore, it may be possible to form the microparticles directly on fingerprint in a 2-stage process.…”

Section: Discussionmentioning

confidence: 99%