Ultrasonication induced nano-emulsification of thyme essential oil: Optimization and antibacterial mechanism against Escherichia coli

Yang, Zhehao; He, Qiao; Ismail, Balarabe B.; Hu, Yaqin; Guo, Mingming

doi:10.1016/j.foodcont.2021.108609

Cited by 35 publications

(22 citation statements)

References 47 publications

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“…Increasing the concentration of negatively charged BM leads to decreasing the ZP while an increase in polysorbate 80 and stearoyl polyoxyl-32 glyceride concentrations as a non-ionic surfactant can cause displacement of OH − or endogenous negatively charged ingredients in the formulation from the particle surface resulting in the net charge reduction. 50 Besides, the effect of sonication time on ZP was not as significant as the other two factors as indicated by its coefficient of 0.9669.…”

Section: Resultsmentioning

confidence: 91%

Development and Optimization of Imiquimod-Loaded Nanostructured Lipid Carriers Using a Hybrid Design of Experiments Approach

Kim¹,

Abdella²,

Abid³

et al. 2023

IJN

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Background Imiquimod (IMQ) is an immunomodulating drug that is approved for the treatment of superficial basal cell carcinoma, actinic keratosis, external genital warts and perianal warts. However, IMQ cream (Aldara ® ) has several drawbacks including poor skin permeation, local toxicity, and compromised patient compliance as a topical pharmacological option. Methods Our research aimed to develop and optimize nanostructured lipid carriers (NLCs) containing IMQ for the first time using a hybrid design of experiments approach. The optimized formulation was then incorporated into a matrix-type topical patch as an alternative dosage form for topical application and evaluated for IMQ deposition across different skin layers in comparison to the performance of the commercial product. Additionally, our work also attempted to highlight the possibility of implementing environment-friendly practices in our IMQ-NLCs formulation development by reviewing our analytical methods and experimental designs and reducing energy and solvent consumption where possible. Results In this study, stearyl alcohol, oleic acid, Tween ® 80 (polysorbate 80), and Gelucire ® 50/13 (Stearoyl polyoxyl-32 glycerides) were selected for formulation development. The formulation was optimized using a 2 k factorial design and a central composite design. The optimized formulation achieved the average particle size, polydispersity index, and zeta potential of 75.6 nm, 0.235, and – 30.9 mV, respectively. Subsequently, a matrix-type patch containing IMQ-NLCs was developed and achieved a statistically significant improvement in IMQ deposition in the deeper skin layers. The IMQ deposition from the patch into the dermis layer and receptor chamber was 3.3 ± 0.9 µg/cm 2 and 12.3 ± 2.2 µg/cm 2 , while the commercial cream only deposited 1.0 ± 0.8 µg/cm 2 and 1.5 ± 0.5 µg/cm 2 of IMQ, respectively. Conclusion In summary, IMQ-NLC-loaded patches represent great potential as a topical treatment option for skin cancer with improved patient compliance.

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Section: Resultsmentioning

confidence: 91%

Development and Optimization of Imiquimod-Loaded Nanostructured Lipid Carriers Using a Hybrid Design of Experiments Approach

Kim¹,

Abdella²,

Abid³

et al. 2023

IJN

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“…In this study, SEM was performed to observe morphological changes in E. coli and S. aureus at 0, 312.5, 625, and 1250 μg/mL [ 37 ]. Briefly, the treated cell suspension was fixed with 2.5% glutaraldehyde for 10 h and dehydrated with different concentrations of ethanol (10, 30, 50, 70, 80, 90, and 100%) for 10 min, followed by drying at the critical point for more than 4 h and spray treatment.…”

Section: Methodsmentioning

confidence: 99%

Potential Application of Luteolin as an Active Antibacterial Composition in the Development of Hand Sanitizer Products

Hou

Wang

et al. 2022

Molecules

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Antibacterial hand sanitizers could play a prominent role in slowing down the spread and infection of hand bacterial pathogens; luteolin (LUT) is potentially useful as an antibacterial component. Therefore, this study elucidated the antibacterial mechanism of LUT against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) and developed an antibacterial hand sanitizer. The results showed that LUT had excellent antibacterial activity against both E. coli (minimum inhibitory concentration (MIC)= 312.5 μg/mL, minimal bactericidal concentration (MBC) = 625 μg/mL), and S. aureus (MIC = 312.5 μg/mL, MBC = 625 μg/mL). Furthermore, LUT induced cell dysfunction in E. coli and S. aureus, changed membrane permeability, and promoted the leakage of cellular contents. Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) analysis showed that LUT treatment affected cell structure and disrupted cell membrane integrity. The Fourier transform infrared analysis (FTIR) also confirmed that the LUT acted on the cell membranes of both E. coli and S. aureus. Overall, the application of LUT in hand sanitizer had better inhibition effects. Therefore, this study could provide insight into expanding the application of LUT in the hand sanitizer markets.

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“…The MBC means the lowest concentration to kill 99.999% of bacteria cells [ 64 ]. An aliquot of 50 µL from negative wells ( Section 5.4.2 ) was spread on Plate Count Agar (PCA) (NEOGEN, Heywood, United Kingdom) and then incubated at 37 °C for 24 h. The MBC value corresponded to the lowest concentration of EOs or their mixture when no colony was observed in the culture medium.…”

Section: Methodsmentioning

confidence: 99%

An Optimization of Oregano, Thyme, and Lemongrass Essential Oil Blend to Simultaneous Inactivation of Relevant Foodborne Pathogens by Simplex–Centroid Mixture Design

et al. 2022

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(1) Background: This study aimed to use the simplex–centroid mixture design methodology coupled with a microdilution assay to predict optimal essential oil (EO) formulations against three potential foodborne pathogens simultaneously through the desirability (D) function. (2) Methods: Oregano (ORE; Origanum vulgare), thyme (THY; Thymus vulgaris), and lemongrass (LG; Cymbopogon citratus) and their blends were evaluated concerning minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for Salmonella enterica serotype Enteritidis, Escherichia coli and Staphylococcus aureus. (3) Results: THY combined with ORE or LG were the most promising EO formulations in inhibiting and killing each bacterium separately. Regarding the simultaneous effect, the optimal proportion for maximum inhibition was composed of 75% ORE, 15% THY, and 10% LG, while for maximum inactivation was 50% ORE, 40% THY, and 10% LG. (4) Conclusion: The multiresponse optimization allowed identifying an EO blend to simultaneously control three potential foodborne pathogens. This first report could be a helpful natural and green alternative for the industry to produce safer food products and mitigate public health risks.

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Ultrasonication induced nano-emulsification of thyme essential oil: Optimization and antibacterial mechanism against Escherichia coli

Cited by 35 publications

References 47 publications

Development and Optimization of Imiquimod-Loaded Nanostructured Lipid Carriers Using a Hybrid Design of Experiments Approach

Development and Optimization of Imiquimod-Loaded Nanostructured Lipid Carriers Using a Hybrid Design of Experiments Approach

Potential Application of Luteolin as an Active Antibacterial Composition in the Development of Hand Sanitizer Products

An Optimization of Oregano, Thyme, and Lemongrass Essential Oil Blend to Simultaneous Inactivation of Relevant Foodborne Pathogens by Simplex–Centroid Mixture Design

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